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Powdery preparation of bacillus pumilus and bdellovibrio and application of powdery preparation

A technology of Bacillus pumilus and leech vibrio, which is applied in the field of feed additives, can solve problems such as unreported, and achieve the effects of inhibiting growth, reducing the occurrence of vibriosis in prawns, and reducing breeding costs.

Inactive Publication Date: 2015-04-22
SUZHOU IRIVET BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But a kind of bacillus pumilus pumilus and Bdellovibrio powder preparation and its application of the present invention that can eliminate the disease caused by vibrio have not been reported yet.

Method used

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  • Powdery preparation of bacillus pumilus and bdellovibrio and application of powdery preparation
  • Powdery preparation of bacillus pumilus and bdellovibrio and application of powdery preparation
  • Powdery preparation of bacillus pumilus and bdellovibrio and application of powdery preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Liquid Formulation

[0026] The liquid preparation of the present invention can be obtained by separately fermenting and culturing Bacillus pumilus and Hirudovibrio in liquid state, and then mixing the concentrated liquids of the two to obtain the liquid preparation of the present invention. The liquid preparation prepared in this example was used in the tests of Example 3, Example 4 and Example 5. The specific preparation method of the present embodiment is as follows:

[0027] Bacillus pumilus strains are cultivated in the order of slant, liquid seed and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): bean cake powder 3% , starch 1.5%, corn steep liquor 1%, glucose 0.5%, (NH 4 ) 2 SO 4 0.1%, NH 4 Cl 0.1%, KH 2 PO 4 0.05%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation culture condition is 28-37℃, the ventilation culture is 36-48h, the number...

Embodiment 2

[0030] Example 2 Powder Formulation

[0031] The powdery preparation of the present invention can be carried out by respectively carrying out solid-state fermentation and culture of Bacillus pumilus and Hirudovibrio, and then mixing the powdered solids of the two; The two are mixed and then dried to obtain a powder formulation. The powder formulation prepared in this example was used in the tests of Example 4 and Example 5. The specific preparation method of the present embodiment is as follows:

[0032] Bacillus pumilus was inoculated into LB medium, cultured at 37°C for 12h, and the bacterial concentration was adjusted to 5×10 11cfu / ml, according to the weight ratio of bacterial liquid: the carrier is 1:1, add carrier corncob powder, and dry at 60℃ for 24h to make the number of viable bacteria ≥5×10 8 cfu / g Bacillus pumilus powder.

[0033] Inoculate leech vibrio strains in 100ml in 1 / 10NB medium, add 100L to a concentration of 1.0×10 6 cfu / ml Escherichia coli bacterial...

Embodiment 3

[0035] Example 3 Analysis of Inhibitory Vibrio Spectrum

[0036] The liquid preparation prepared in Example 1 was subjected to Vibrio inhibition spectrum analysis by the flat filter paper diffusion method. The strength of the antibacterial ability is determined according to the size of the inhibition zone, and the results of the antibacterial spectrum analysis are shown in Table 1:

[0037] Table 1 Analysis of Vibrio-inhibitory Activity of Compositions

[0038]

[0039] Note: +++: strong bacteriostatic activity; ++: moderate bacteriostatic activity; +: bacteriostatic activity.

[0040] As can be seen from Table 1, from the analysis of common pathogenic Vibrio, the liquid preparations of Bacillus pumilus and Vibrio leech of the present invention have broad-spectrum inhibitory activity against Vibrio.

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Abstract

The invention relates to a powdery preparation of bacillus pumilus and bdellovibrio and belongs to the technical field of feed additives. The powdery preparation contains the bacillus pumilus and the bdellovibrio of which the preservation number is CCTCC M 2013240, wherein the bdellovibrio is one or more of bdellovibrio bacteriovorus, bdellovibrio stolpii and bdellovibrio starrii. The invention also provides application of the powdery preparation in preparation of a feed additive for treating a disease caused by vibrio. The powdery preparation has the advantages of effectively treating the disease caused by the vibrio in a broad-spectrum manner, inhibiting the growth of pathogenic vibrio in the intestinal tract of a prawn and reducing occurrence of a vibrio disease of the prawn when being added into a feed for the prawn, also promoting the growth of the prawn, lowering the cultivation cost and being worthy of wide application in aquaculture.

Description

technical field [0001] The invention relates to the technical field of feed additives, in particular to the powdery preparations of Bacillus pumilus and Hirudovibrio and their application. Background technique [0002] Vibrio belongs to the genus Vibrio of the Vibrio family, and is a class of short rod-shaped bacteria with gram-negative, polar flagella, motile, and no spores. Not obvious. Vibrio is one of the dominant groups of marine bacteria. It is widely distributed in various estuaries, inner bays, coasts, aquaculture areas and water bodies and sediments in open seas around the world. Marine Vibrio occupies an important ecological position in the marine environment, has a variety of physiological and ecological functions, plays an important role in the marine micro-food ring, and plays a very important role in the cycle of marine nutrients. But at the same time, Vibrio is an important class of pathogenic bacteria in aquatic animals. [0003] With the rapid development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/08C12R1/63
CPCC12N1/20
Inventor 宋增福许维素
Owner SUZHOU IRIVET BIOTECH
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