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Hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry and application thereof

A technology of glycidyl ether and hyperbranched polymerization, which is used in measurement devices, instruments, scientific instruments and other directions to achieve the effect of reducing non-specific adsorption and strong hydrophilicity

Active Publication Date: 2015-04-22
JILIN GETEIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report about hyperbranched polyglycidyl ether modified latex microspheres in enhancing immune turbidimetry

Method used

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  • Hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry and application thereof
  • Hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry and application thereof
  • Hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry and application thereof

Examples

Experimental program
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Embodiment 1

[0058] Example 1 Preparation method of hyperbranched polyglycidyl ether modified polystyrene latex microspheres-detecting molecular diagnostic reagents

[0059] 1. Preparation of hyperbranched polyglycidyl ether modified polystyrene microspheres:

[0060] method one:

[0061] Preparation of hyperbranched polyglycidyl ether: In a three-necked flask equipped with mechanical stirring and a micro constant flow pump, add 0.24g trimethylolpropane (TMP) as the initiator of the reaction, using 3.7M CH 3 OK (dissolved in methanol) partially deprotonated the hydroxyl group of TMP, the deprotonated part accounted for 10% of the total part, and the excess methanol was removed by heating and evaporating. Then 50mL of glycidol was slowly added dropwise into the system to initiate polymerization, pay attention to the drop rate of glycidol, as dropping too fast may cause implosion. The reaction temperature was 95°C, and the reaction was carried out for 12 hours. After the reaction, the prod...

Embodiment 2

[0070] In Example 2, the commercialized carboxypolystyrene latex microspheres (Nanjing Jidan Biopharmaceutical Co., Ltd., product number PM023) used as a control reagent were coupled to the CRP antibody in the same way as above, and only commercial carboxypolystyrene microspheres were used instead of hyperbranched polystyrene microspheres. Glycidyl ether modified polystyrene microspheres.

[0071] Example 2 Hyperbranched polyglycidyl ether modified polystyrene latex microspheres enhanced immune turbidimetric method and polystyrene latex microspheres enhanced immune turbidimetric method comparison

[0072] Add 30 μl of hyperbranched polyglycidyl ether-modified polystyrene latex microspheres to CRP antibody complex to 100 μl of the sample to be analyzed, and react for 15 minutes. Due to the specific immune reaction, hyperbranched polyglycidyl ether-modified polyphenylene Vinyl latex microspheres ~ CRP antibody ~ CRP antigen immune complex, with a biochemical instrument (Hitachi ...

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Abstract

The invention discloses hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry and an application thereof. A latex microsphere material which is hydrophilic on surface and is rich in functional groups is prepared by using the hyperbranched polyglycerol modified latex microspheres. In actual use, when an immunoturbidimetric reagent of the hyperbranched polyglycerol modified latex microspheres is used, the reagent can be used for inhibiting nonspecific adsorption of proteins well and the antibody coupling rate is further higher than that of common commercialized latex microspheres. In project performance analysis, the latex microsphere reagent can be used for greatly enhancing reaction between antigens and antibodies. Compared with common commercialized latex microsphere reagents, the latex microsphere reagent has the advantages that the sensitivity and detection range are obviously improved and the hyperbranched polyglycerol modified latex microsphere enhanced immunoturbidimetry has excellent performances of relevance, stability and repeatability.

Description

technical field [0001] The invention belongs to the field of biological detection reagents, and relates to a hyperbranched polyglycidyl ether modified latex microsphere enhanced immune turbidimetry and an application thereof. Background technique [0002] Immunoturbidimetric Assays is a dynamic assay for antigen-antibody binding. The basic principle is: when the antigen and the antibody react in a special dilution system and the ratio is appropriate (generally, the antibody is excessive), the soluble immune complex formed is under the action of the polymerization accelerator (polyethylene glycol, etc.) in the dilution system. , It precipitates from the liquid phase to form particles, which makes the reaction solution appear turbid. When the antibody concentration is fixed, the amount of the formed immune complex increases with the increase of the amount of antigen in the test sample, and the turbidity of the reaction solution also increases accordingly. By measuring the tu...

Claims

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Application Information

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IPC IPC(8): G01N33/546G01N33/531
CPCG01N33/54346G01N33/54393
Inventor 金晶罗雅赛朱宗哲童鎏苏恩本
Owner JILIN GETEIN BIOTECH CO LTD
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