Double imaging polylactic acid-glycolic acid copolymer particles and its preparation method and application

Abstract

The invention provides poly(lactic-co-glycolic acid) particles for dual imaging, as well as a preparation method and application of the poly(lactic-co-glycolic acid) particles. The preparation method comprises the following steps: dissolving poly(lactic-co-glycolic acid) in an organic solvent to prepare a solution A with the concentration of 0.005-0.1 g / mL; adding 0.005-0.2 mL of liquid fluorocarbon into the solution A to obtain a solution B; dissolving methylene blue in water to obtain a solution C with the concentration of 0.01-3 mmol / L; dissolving a surfactant in water to obtain a solution D with the quality concentration of 0.2-3%; adding the solution C into the solution B to obtain an emulsion E; dripping the emulsion E into the solution D to obtain an emulsion F; stirring the emulsion F till the organic solvents of the emulsion F are completely volatilized, and carrying out dialysis and purification to obtain the poly(lactic-co-glycolic acid) particles. The prepared poly(lactic-co-glycolic acid) particles can be used as a contrast agent for ultrasonic and photo-acoustic dual-functional imaging for clinical use.

Description

technical field [0001] The invention relates to a polylactic acid-glycolic acid copolymer particle and its preparation method and application, in particular to a polylactic acid-glycolic acid copolymer particle for ultrasonic and photoacoustic dual imaging and its preparation method and application, belonging to the technical field of biomedical imaging . Background technique [0002] Photoacoustic imaging is a technique that utilizes thermal expansion of a material after absorbing light energy to generate sound waves for imaging. Photoacoustic imaging has the advantages of high resolution and large penetration depth. Since photoacoustic imaging uses acoustic probes for signal collection, it can be integrated with traditional ultrasound imaging to provide more comprehensive diagnostic information. [0003] Because hemoglobin and melanin in the body have strong light absorption, they are often used for angiography and melanoma imaging. But these two contrast substances can...

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Problems solved by technology

However, since methylene blue is prone to generate reactive oxygen species-oxidized phospholipids, thereby destroying microvesicles

And existing report (Langmuir 2007,23,1307-1314) confirms that methylene blue can increase liposome surface tension, makes liposome stability worse, in addition, adopts phospholipid to wrap methylene blue and then pass into gaseous fluorocarbon again, because gas Blocked by the phospholipid layer, the permeability is poor, and there are fewer fluorocarbon molecules that can enter the phospholipid layer. In addition, the stability of the phospholipid layer that wraps the gas is not as stable as that of the liquid substance

[0006] There are also micro-droplets using gold nanomaterials as light-absorbing substances, which cause unstable absorption peaks due to the easy thermal melting of gold particles; micro-droplets using indocyanine green as light-absorbing substances, due to indocyanine green It is very sensitive to the environment and easily destroyed; liposomes are used to wrap microbubbles of methylene blue, because methylene blue will destroy liposomes, causing liposomes to be unstable; light-absorbing substances in other microdroplets are biocompatible Sex is not ideal

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