Amphikrikos marinus as well as application and culture method thereof

A technology of Pseudomonas bisporus and a cultivation method, applied in the field of marine Pseudomonas bisporus and its application, and new marine Pseudomonas algae strains, can solve problems such as high cost, achieve high oil content and growth rate, reduce The effect of production costs

Inactive Publication Date: 2015-04-29
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the resources of solar energy are inexhaustible, but because of the high cost of development and utilization, it is still impossible to develop rapidly and be widely used in a short period of time.

Method used

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  • Amphikrikos marinus as well as application and culture method thereof
  • Amphikrikos marinus as well as application and culture method thereof

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Experimental program
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Embodiment 1

[0018] A marine Pseudobisporus, isolated from the seawater of the Yellow Sea, classified as Pseudobisporus Amphikrikos marinus, was preserved in the General Microorganism Center of China Microbiology Culture Collection Management Committee on October 22, 2014, strain preservation The registration number is CGMCC No. 9814. The collection method of the Pseudobisporus is that the phytoplankton is taken off the net; the separation method adopts micro-pipe suction, step-by-step dilution separation and plate coating method.

[0019] The cultivation method, the specific operation steps are as follows: microalgae are enriched and cultivated in seawater F / 2 medium, and the initial inoculation density is 1×10 5 pcs mL -1 , placed in an artificial climate box with a light-to-dark ratio of 14h:10h, a light intensity of 1100lux, a temperature of 25°C, and a relative humidity of 70% to 80% for 2 weeks, and centrifuged marine Pseudomonas algae liquid. Harvest Pseudomonas bisporus.

[002...

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Abstract

The invention discloses amphikrikos marinus as well as an application and a culture method thereof. The amphikrikos marinus is separated from the seawater of the Yellow Sea and classified and named amphikrikos marinus and collected in the China General Microbiological Culture Collection Center on October 22, 2014; and the collection number of the strain is CGMCC No.9814. A collection method of the amphikrikos marinus is to enable phytoplankton to get rid of the net; and a separation method adopts a micropipette for absorption, step-by-step dilution and separation and a spread-plate method. The amphikrikos marinus disclosed by the invention has the beneficial effects that compared with other algae species serving as a biodiesel raw material, the amphikrikos marinus has remarkably high fat content and growth speed, and the production cost of biodiesel can be effectively reduced.

Description

technical field [0001] The invention belongs to the field of bioenergy, and relates to a marine Pseudobisporus and its application. More specifically, the present invention relates to a new marine Pseudobisporus strain, which has a high oil content and can be used to prepare biodiesel, feed or cooking oil. Background technique [0002] As the fossil energy reserves on the earth are decreasing day by day, oil resources are likely to be depleted rapidly in a short period of time. The reserves of fossil energy are not inexhaustible, and there are very few fossil energy reserves that are easy to exploit and utilize. The remaining reserves lose the value of continued mining due to the increasing difficulty of development. Under the circumstances that the world's energy consumption is dominated by oil, if the energy consumption structure remains unchanged, a new generation of energy crisis will occur. Although coal resources are larger than oil reserves, they are not inexhaustib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/649C12N1/125C12R2001/89Y02E50/10
Inventor 韩吉昌张琳王松潘克厚
Owner OCEAN UNIV OF CHINA
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