A kind of polymer phospholipid nanoparticle loaded with icg and preparation method thereof
A nanoparticle and polymer technology, which is applied in the directions of non-active ingredient medical preparations, medical preparations containing active ingredients, and drug combinations, can solve the problems of small preparation dose, achieve stable properties, and simple and easy preparation methods. , the effect of uniform particle size
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Embodiment 1
[0029] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:
[0030] (1) Dissolving soybean lecithin and ICG in 4% ethanol aqueous solution to obtain a mixed solution, the concentration of soybean lecithin in the mixed solution is 0.15mg / mL, the concentration of ICG is 0.2mg / mL, and the total volume is 40mL ; Keep the mixed solution at a temperature of 90° C., adjust the pressure to 500 bar, and initially emulsify for 2 minutes to obtain an emulsion;
[0031] (2) Dissolve polymer PLGA with a molecular weight of 5000 in acetocyanide to obtain a PLGA solution with a concentration of 1 mg / mL, with a total volume of 10 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 90°C, and keep the pressure at 500 bar, The emulsification was continued for 4 minutes to form a 50 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticles.
[0032] figure...
Embodiment 2
[0035] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:
[0036] (1) soybean lecithin and ICG are dissolved in the ethanol aqueous solution of 4% to obtain mixed solution in mass concentration, the concentration range of soybean lecithin in the mixed solution is 0.75mg / mL, ICG concentration is 1.5mg / mL, and total volume is 40mL; keep the mixed solution at a temperature of 60°C, adjust the pressure to 1400bar, and emulsify initially for 2 minutes to obtain an emulsion;
[0037] (2) Dissolve polymer PLGA with a molecular weight of 15,000 in acetocyanide to obtain a PLGA solution with a concentration of 5 mg / mL, with a total volume of 10 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 60 °C, and the pressure at 1400 bar, The emulsification was continued for 6 minutes to form a 50 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticl...
Embodiment 3
[0039] A preparation method of polymer-phospholipid nanoparticles loaded with ICG, comprising the following steps:
[0040] (1) Dissolving soybean lecithin and ICG in 4% ethanol aqueous solution to obtain a mixed solution, the concentration of soybean lecithin in the mixed solution is 0.15mg / mL, the concentration of ICG is 0.2mg / mL, and the total volume is 800mL ; Keep the mixed solution at a temperature of 80°C, adjust the pressure to 500bar, and initially emulsify for 2 minutes to obtain an emulsion;
[0041] (2) Dissolve polymer PLGA with a molecular weight of 10,000 in acetonitrile to obtain a PLGA solution with a concentration of 1 mg / mL, with a total volume of 200 mL; add the PLGA solution dropwise to the emulsion, keep the temperature at 80°C, and the pressure at 500 bar, The emulsification was continued for 4 minutes to form a 1000 mL solution containing ICG-loaded PLGA-phospholipid nanoparticles to obtain ICG-loaded PLGA-phospholipid nanoparticles. The particle size ...
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