Ultralow temperature detoxification method for strawberry stem tip

An ultra-low temperature, shoot tip technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as blanks, and achieve the effects of short time consumption, high detoxification rate, good technical effects and promotion prospects.

Inactive Publication Date: 2015-05-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research of this technology on strawberries is almost blank, and Sichuan is a major province of winter strawberry production in my country, so it is imperative to establish strawberry ultra-low temperature therapy detoxification technology

Method used

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  • Ultralow temperature detoxification method for strawberry stem tip
  • Ultralow temperature detoxification method for strawberry stem tip
  • Ultralow temperature detoxification method for strawberry stem tip

Examples

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Embodiment 1

[0022] The invention provides a method for ultra-low temperature detoxification of strawberry stem tips, which specifically includes the following steps:

[0023] Peel off 2mm strawberry stem tips and transfer to pre-medium (MS medium + 2% glycerol + 3M sucrose + 6.5g L -1 , pH5.8), pre-cultured at 4°C in the dark, with a sucrose concentration of 0.3-0.5mol L -1 , the pre-cultivation time is set to 3-5 days, and the shoot tips after pre-cultivation are placed in 60% PVS at 25°C 2 Medium (PVS 2 Medium: MS medium + 30% glycerol + 15% ethylene glycol + 15% dimethyl sulfoxide + 40g L -1 Sucrose, 60% PVS 2 Medium: 0.6mol·l -1 Sucrose MS liquid medium and PVS 2 The volume ratio is 40:60) and transferred to 100% PVS after loading for 30-90min 2 In the culture medium solution, treat at 0°C for 1-3 hours, then transfer to a 1.5ml cryopreservation tube, put it into liquid nitrogen for 1 hour, quickly transfer the cryopreservation tube to 40°C water, and thaw the shoot tips in a wa...

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Abstract

The invention discloses an ultralow temperature detoxification method for a strawberry stem tip, and belongs to the technical field of strawberry detoxification. The ultralow temperature detoxification method for the strawberry stem tip specifically comprises the following steps: taking a strawberry explant contracting the virus, cutting off the stem tip, and pre-culturing; feeding liquid nitrogen, thawing and washing; restoring culture and plant regeneration; and detecting pathogens of the regenerated plant. The ultralow temperature cold therapy disclosed by the invention is applied to the field of strawberry detoxification; the ultralow temperature detoxification method for the strawberry stem tip is built; and the method has the advantages of being simple to operate, short in elapsed time and high in detoxification rate. The ultralow temperature storage survival rate of the ultralow temperature detoxification method for the strawberry stem tip is 69.03%; the detoxification rate is 100%; and the ultralow temperature detoxification method has good technical effect and the popularization prospect.

Description

technical field [0001] The invention belongs to the technical field of detoxification of strawberries, in particular to a method for detoxification of strawberry stem tips at ultra-low temperature. Background technique [0002] Strawberry (Fragaria×ananassa Duch.) is a perennial evergreen herbaceous plant of the Rosaceae (Rosaceae) genus Fragaria. The fruit is tender and juicy, bright in color, pleasant in aroma, and moderately sweet and sour in taste. It is one of the traditional excellent berry fruits in my country. Strawberry fruit is rich in vitamin C, organic acids, sugar, minerals and pectin and other nutrients. In recent years, studies have found that biologically active substances such as ellagitannins, anthocyanins and proanthocyanidins contained in strawberries can prevent the occurrence of cardiovascular and cerebrovascular diseases and cancer, so strawberries are favored by consumers. [0003] Strawberry reproduction often adopts traditional stolon burying or b...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 罗娅汤浩茹邱静李雁翎田钰冉谢然夫凌亚杰王小蓉张勇陈清
Owner SICHUAN AGRI UNIV
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