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Method for detecting concentration of Cu<2+> based on bovine serum albumin functionalized gold nanocluster light scattering probe

A gold nanocluster, bovine serum albumin technology, applied in the field of nanomaterial applications, can solve the problems of background signal interference, fluorescence quenching, narrow linear range, etc., to achieve no toxic side effects, low detection limit, and wide linear range. Effect

Active Publication Date: 2015-05-13
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods still have some limitations, such as inductively coupled plasma usually needs to be combined with other techniques, atomic absorption spectroscopy usually has a narrow linear range, fluorescence quenching studies background signal interference, etc.

Method used

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  • Method for detecting concentration of Cu&lt;2+&gt; based on bovine serum albumin functionalized gold nanocluster light scattering probe
  • Method for detecting concentration of Cu&lt;2+&gt; based on bovine serum albumin functionalized gold nanocluster light scattering probe
  • Method for detecting concentration of Cu&lt;2+&gt; based on bovine serum albumin functionalized gold nanocluster light scattering probe

Examples

Experimental program
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Embodiment 1

[0021] Example 1 The bovine serum albumin functionalized gold nanocluster (BSA-Au NCs) probe was synthesized, and the concentration of copper ions was detected by resonance light scattering.

[0022] 1. Synthesis of bovine serum albumin functionalized gold nanoclusters (BSA-Au NCs) light scattering probe:

[0023] Bovine serum albumin-functionalized gold nanoclusters (BSA-Au NCs) were synthesized by bovine serum albumin (BSA)-reduced chloroauric acid (HAuCl 4 4H 2 0) Synthetic. Add 10ml of 50mg / ml bovine serum albumin (BSA) and 4.1ml of chloroauric acid (1%) into a round-bottomed flask, and stir magnetically at 37°C for 2 min. Then continue to add 1ml of 1M sodium hydroxide, the color of the solution changes from light yellow to brown in an instant and then turns into light yellow after stirring. Stirring was continued for 12 hours, and finally a light brown aqueous solution of bovine serum albumin-functionalized gold nanoclusters (BSA-Au NCs) was obtained, and the solut...

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Abstract

The invention discloses a method for quickly detecting the concentration of copper ions based on a gold nanocluster light scattering probe. The method comprises the following steps: preparing a bovine serum albumin functionalized gold nanocluster (BSA-Au NCs) light scattering probe which can be used for quantitatively detecting the concentration of the copper ions; and causing clustering under the interaction between the copper ions and the bovine serum albumin functionalized gold nanocluster so as to implement quantitative detection of the copper ions. The light scattering detection probe is easy to prepare and save; all the reagents are free of toxic and side effects. The light scattering detection method has the characteristics of high sensitivity, low detection limit, wide linear range, simplicity and convenience, and easiness in operation.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial application, in particular to a fast and sensitive detection of Cu based on a gold nanocluster light scattering probe. 2+ A new approach to concentration. Background technique [0002] Recently, gold nanosensors have attracted widespread attention due to their facile and real-time tracking of metal ions in the environment. However, most existing gold sensors still use fluorescence detection. Resonance Light Scattering (RLS) This new technique produces signals primarily due to aggregation of chromophores. So far, the work of using bovine serum albumin as a template to synthesize fluorescent nano-gold clusters for the detection of copper ions by light scattering has not been reported. [0003] Copper ions are essential elements for animals or plants. Copper ions are widely distributed in organisms, mostly in the form of organic compounds. Copper deficiency in the body may trigger various di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 冯大千朱文洁王伟
Owner YANCHENG INST OF TECH
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