Vaccine for preventing streptococcus agalactiae disease of golden pomfret

A technology for streptococcus nisca and golden pomfret, applied in the directions of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of increasing the cost of breeding medicinal products, breeding cycle, high mortality, and increasing breeding costs, and achieves prevention. Streptococcus agalactiae, good application prospect, good economic effect

Inactive Publication Date: 2015-05-20
TONGWEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, with the increase in breeding volume and scale, disease outbreaks of golden pompano have gradually increased, especially in the past two years, a new disease has occurred in golden pomfret breeding areas in Hainan. The disease of fin ray congestion and bleeding and anal swelling. After dissection, it was found that some livers were white, some livers were severely congested, there was a lot of white mucus in the intestine, and the symptoms of congestion on the inner wall of the intestine were typical symptoms. The characteristics of high mortality have brought greater economic losses to farmers
[0004] Since the cause of the disease is still unclear, at present, general-purpose fish disease prevention and control drugs can only be used for preventive treatment, but the control effect is not obvious. The cycle will inevitably bring about an increase in the cost of breeding

Method used

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  • Vaccine for preventing streptococcus agalactiae disease of golden pomfret
  • Vaccine for preventing streptococcus agalactiae disease of golden pomfret
  • Vaccine for preventing streptococcus agalactiae disease of golden pomfret

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Isolation and Identification of Bacterial Strains

[0025] 1. Experimental materials and methods

[0026] 1.1 Experimental materials

[0027] The bacteria to be identified were isolated from a diseased golden pomfret in a farm in Lingao, Hainan. The isolation medium was BHI, the culture temperature was 37°C, and the serial number was TW2014-48.

[0028] Reagent Consumables 16S rDNA Universal Amplification Kit (TAKARA), BHI Medium, Bacterial Biochemical Trace Identification Tube (Hangzhou Tianhe Biological Reagent Co., Ltd.), Taq DNA Polymerase (TAKARA), Agarose (TianGen), 100bp DNA maker ( Tiangen).

[0029] 1.2 Experimental method

[0030] 1.2.1 Gram stain observation

[0031] Take a fresh slant cultivated overnight, pick a small amount of bacteria with an inoculation loop, apply it on a glass slide dripped with a small amount of sterile saline, perform Gram staining, and observe the staining results under a microscope.

[0032] 1.2.2 Amplification and a...

Embodiment 2

[0049] Example 2 Preparation of inactivated vaccine against golden pomfret Streptococcus agalactiae (TW2014-48) and study on its immune effect

[0050] In this experiment, the preparation of the inactivated whole vaccine and the research on the immune effect of Streptococcus agalactiae isolated from naturally occurring golden pomfret were carried out, aiming to provide theoretical and experimental basis for the immune prevention and treatment of Streptococcus agalactiae in golden pomfret .

[0051] 1. Materials and methods

[0052] 1.1 Materials

[0053] Streptococcus agalactiae (TW2014-48) was isolated from a diseased golden pomfret in a farm in Lingao, Hainan, and was preserved in the China General Microorganism Culture Collection Management Center, with the preservation number: CGMCC NO.9974.

[0054] Healthy golden pompano was purchased from a farm in Hainan, with an average weight of 153.2±3.5g, and was domesticated in an aquarium for 10 days before the experiment.

[...

Embodiment 3

[0075] Example 3 Comparative study on the immune effect of the inactivated whole-bacteria vaccine prepared by golden pomfret Streptococcus agalactiae (TW2014-48) and the standard strain of Streptococcus agalactiae (ATCC51487)

[0076] In this experiment, a comparative study was carried out on the immune effect of isolated Streptococcus agalactiae and the inactivated vaccine prepared by the standard strain on golden pomfret streptococcus agalactiae, which provided a reliable basis for better immune prevention and treatment of golden pomfret streptococcus agalactiae in accordance with.

[0077] 1. Materials and methods

[0078] 1.1 Strains

[0079] Golden Pomfret Streptococcus agalactiae (TW2014-48) was isolated from the diseased Golden Pomfret, and the preservation number: CGMCC NO.9974.

[0080] The standard strain of Streptococcus agalactiae (ATCC 51487) was donated by the Fish Disease Research Center of Sichuan Agricultural University.

[0081] 1.2 Experimental fish

[008...

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Abstract

The invention provides an application of streptococcus agalactiae in preparation of medicines for preventing a streptococcus agalactiae disease of golden pomfret, and further provides a vaccine. The vaccine is prepared from the streptococcus agalactiae and pharmaceutically acceptable auxiliary materials or carriers. The invention further provides an application of the vaccine in preparation of medicines for preventing the streptococcus agalactiae disease of golden pomfret. The vaccine provided by the invention is capable of stimulating the golden pomfret to generate the immunity of resisting the streptococcus agalactiae disease of the golden pomfret. The streptococcus agalactiae disease of the golden pomfret can be effectively prevented.

Description

technical field [0001] The invention relates to a fish vaccine, in particular to a vaccine for preventing agalactiae streptococcosis of golden pomfret. Background technique [0002] The scientific name of golden pomfret is oval pomfret, and the local name is yellow wax pomfret and golden pomfret. It has the characteristics of delicious meat and rich nutrition, and is very popular among consumers. Therefore, golden pomfret, as a main mariculture species, has developed rapidly in my country's coastal areas, especially in Guangdong and Hainan, and its aquaculture scale and aquaculture output have all increased year by year. [0003] However, with the increase in breeding volume and scale, disease outbreaks of golden pompano have gradually increased, especially in the past two years, a new disease has occurred in golden pomfret breeding areas in Hainan. The disease of fin ray congestion and bleeding and anal swelling. After dissection, it was found that some livers were white,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61P31/04A61K47/18C12N1/20C12R1/46
Inventor 阳涛刘天强王浩丞敬小兵黄冠军黄凌远
Owner TONGWEI
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