Vaccine for preventing streptococcus agalactiae disease of golden pomfret
A technology for streptococcus nisca and golden pomfret, applied in the directions of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of increasing the cost of breeding medicinal products, breeding cycle, high mortality, and increasing breeding costs, and achieves prevention. Streptococcus agalactiae, good application prospect, good economic effect
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Embodiment 1
[0024] Example 1 Isolation and Identification of Bacterial Strains
[0025] 1. Experimental materials and methods
[0026] 1.1 Experimental materials
[0027] The bacteria to be identified were isolated from a diseased golden pomfret in a farm in Lingao, Hainan. The isolation medium was BHI, the culture temperature was 37°C, and the serial number was TW2014-48.
[0028] Reagent Consumables 16S rDNA Universal Amplification Kit (TAKARA), BHI Medium, Bacterial Biochemical Trace Identification Tube (Hangzhou Tianhe Biological Reagent Co., Ltd.), Taq DNA Polymerase (TAKARA), Agarose (TianGen), 100bp DNA maker ( Tiangen).
[0029] 1.2 Experimental method
[0030] 1.2.1 Gram stain observation
[0031] Take a fresh slant cultivated overnight, pick a small amount of bacteria with an inoculation loop, apply it on a glass slide dripped with a small amount of sterile saline, perform Gram staining, and observe the staining results under a microscope.
[0032] 1.2.2 Amplification and a...
Embodiment 2
[0049] Example 2 Preparation of inactivated vaccine against golden pomfret Streptococcus agalactiae (TW2014-48) and study on its immune effect
[0050] In this experiment, the preparation of the inactivated whole vaccine and the research on the immune effect of Streptococcus agalactiae isolated from naturally occurring golden pomfret were carried out, aiming to provide theoretical and experimental basis for the immune prevention and treatment of Streptococcus agalactiae in golden pomfret .
[0051] 1. Materials and methods
[0052] 1.1 Materials
[0053] Streptococcus agalactiae (TW2014-48) was isolated from a diseased golden pomfret in a farm in Lingao, Hainan, and was preserved in the China General Microorganism Culture Collection Management Center, with the preservation number: CGMCC NO.9974.
[0054] Healthy golden pompano was purchased from a farm in Hainan, with an average weight of 153.2±3.5g, and was domesticated in an aquarium for 10 days before the experiment.
[...
Embodiment 3
[0075] Example 3 Comparative study on the immune effect of the inactivated whole-bacteria vaccine prepared by golden pomfret Streptococcus agalactiae (TW2014-48) and the standard strain of Streptococcus agalactiae (ATCC51487)
[0076] In this experiment, a comparative study was carried out on the immune effect of isolated Streptococcus agalactiae and the inactivated vaccine prepared by the standard strain on golden pomfret streptococcus agalactiae, which provided a reliable basis for better immune prevention and treatment of golden pomfret streptococcus agalactiae in accordance with.
[0077] 1. Materials and methods
[0078] 1.1 Strains
[0079] Golden Pomfret Streptococcus agalactiae (TW2014-48) was isolated from the diseased Golden Pomfret, and the preservation number: CGMCC NO.9974.
[0080] The standard strain of Streptococcus agalactiae (ATCC 51487) was donated by the Fish Disease Research Center of Sichuan Agricultural University.
[0081] 1.2 Experimental fish
[008...
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