Method for extracting auricularia polytricha polysaccharide

An extraction method and Auricularia auricularia technology are applied in the field of extraction of Auricularia auricularia polysaccharide, which can solve the problems of destroying polysaccharide components and active structures, being unsuitable for large-scale extraction applications, wasting time and raw materials, etc. Industrial production, the effect of reducing energy consumption

Inactive Publication Date: 2015-05-20
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method has many times of extraction, low yield, long time, and waste of time and raw materials
Acid leaching and alkali leaching are easy to hydrolyze some polysaccharides, destroying the composition and active structure of polysaccharides, reducing the yield of polysaccharides, and are not suitable for large-scale extraction applications

Method used

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  • Method for extracting auricularia polytricha polysaccharide
  • Method for extracting auricularia polytricha polysaccharide
  • Method for extracting auricularia polytricha polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] (1) Take the Auricularia auricula fruit body and grind it after drying to obtain the Auricularia auricula powder, the particle size of the Auricularia auricula powder is 160-300 mesh;

[0067] (2) Mix the Auricularia auricula powder obtained above with distilled water in a mass ratio of 1:60, and stir evenly;

[0068] (3) Extract the mixed solution in step (2) for 20 minutes under the conditions of 120°C and 0.12MP in a pressure steamer;

[0069] (4) centrifuging the Auricularia auricula extract in step (3) and separating the supernatant and the sediment, and getting the supernatant;

[0070] (5) After the supernatant of step (4) is deproteinized by the Sevage method, the polysaccharide of Auricularia trifoliata is obtained. The extraction rate of Auricularia polysaccharide is 13.35%.

Embodiment 2

[0072] (1) Take the Auricularia auricula fruit body and grind it after drying to obtain the Auricularia auricula powder, the particle size of the Auricularia auricula powder is 160-300 mesh;

[0073] (2) Mix the Auricularia auricula powder obtained above with distilled water in a mass ratio of 1:60, and stir evenly;

[0074] (3) Extract the mixed solution in step (2) for 80 minutes under the conditions of 120° C. and 0.12 MP in a pressure steamer;

[0075] (4) centrifuging the Auricularia auricula extract in step (3) and separating the supernatant and the sediment, and getting the supernatant;

[0076] (5) After the supernatant of step (4) is deproteinized by the Sevage method, the polysaccharide of Auricularia trifoliata is obtained. The extraction rate of Auricularia polysaccharides was 19.67%.

Embodiment 3

[0078] (1) Take the Auricularia auricula fruit body and grind it after drying to obtain the Auricularia auricula powder, the particle size of the Auricularia auricula powder is 100-160 mesh;

[0079] (2) Mix the Auricularia auricula powder obtained above with distilled water in a mass ratio of 1:40, and stir evenly;

[0080] (3) Extract the mixed solution in step (2) for 80 minutes under the conditions of 120°C and 0.12MP in a pressure steamer;

[0081] (4) centrifuging the Auricularia auricula extract in step (3) and separating the supernatant and the sediment, and getting the supernatant;

[0082] (5) After the supernatant of step (4) is deproteinized by the Sevage method, the polysaccharide of Auricularia trifoliata is obtained. The extraction rate of Auricularia auricula polysaccharide is 27.27%.

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Abstract

The invention belongs to the field of fungus application, relates to an efficient method for extracting auricularia polytricha polysaccharide, and aims to increase the yield of auricularia polytricha polysaccharide. The method comprises the following steps: (1) crushing dried auricularia polytricha until the particle size is 60-300 meshes, thereby obtaining auricularia polytricha powder; (2) mixing the auricularia polytricha powder obtained in the step (1) with water in a mass ratio of 1:(20-100), and uniformly stirring; (3) performing digestion for at least 20 minutes in a sealed container at the temperature of at least 120 DEG C under the pressure of at least 0.12MPa by using a hot-pressing method, thereby obtaining an auricularia polytricha extract; and (4) centrifuging the auricularia polytricha extract, and removing protein from the supernate by using a Sevage method, thereby obtaining auricularia polytricha polysaccharide. By adopting the method, the extraction yield of auricularia polytricha polysaccharide is increased, the extraction time is greatly shortened, the energy consumption and the production cost are greatly reduced, and the method is simple and rapid in process route, easy in industrial production and an effective method for comprehensive processing and utilization of auricularia polytricha.

Description

technical field [0001] The invention belongs to the field of fungi utilization, and relates to a high-efficiency extraction method of fungus polysaccharide. Background technique [0002] Auricularia polytricha (Mont.) Sacc.], also known as white-backed fungus and yellow-backed fungus, belongs to Basidiomycota, Laminaria, Auricularia, Auriculariaceae, and the genus Auricularia. The back of the fungus fruit body is rich in fluff layer, rich in nutrients, protein, fat, carbohydrates, etc. are similar to black fungus, and some chemical components are higher than black fungus, known as "black health food" and "tree jellyfish". Invigorate qi and blood, lower blood viscosity, lower blood fat, clear lung and moisten dryness, cleanse stomach and intestines, laxative lower blood pressure, reduce calculus, antithrombotic, prevent atherosclerosis, stroke and myocardial infarction, etc. In the patent "A kind of Auricularia officinalis extract and its preparation method and application",...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 黄文丽陈祖琴
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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