Research on method for extracting oil and fat from soybeans through enzymolysis by using gas-liquid-solid three-phase magnetically-stabilized fluidized bed

Abstract

The invention provides a method for extracting oil and fat from soybeans through enzymolysis by using a gas-liquid-solid three-phase magnetically-stabilized fluidized bed. Innovation lies in that protease is immobilized on a magnetic carrier, can be regularly distributed by using a magnetic field generated by the magnetically-stabilized fluidized bed and can be of magnetically-stabilized distribution, and then, the soybeans can be subjected to full enzymolysis by the protease so as to extract oil and fat. The method comprises the steps of crushing extruded soybeans, which serve as a raw material, mixing the crushed soybeans and water so as to form a mixture, preheating the mixture to a specified temperature, then, enabling the preheated mixture to enter the bottom of a gas-liquid-solid three-phase magnetically-stabilized fluidized-bed reactor, adding magnetic immobilized protease into a gas-liquid-solid three-phase magnetically-stabilized fluidized bed, enabling the mixture, formed by the soybean powder and the water, and the magnetic immobilized protease to be subjected to reaction and enzymolysis, extracting soybean oil through high-speed centrifugal separation after enzymolysis, inspecting the influence on oil and fat extraction rate caused by the protease dosage, reaction temperature, pH value and mixed material-liquid ratio of the magnetic immobilized protease in the magnetically-stabilized fluidized-bed reactor by taking oil and fat extraction rate as a reference index, and searching reaction parameters of the magnetically-stabilized fluidized-bed reactor.

Description

technical field [0001] The invention relates to the field of grain oil processing, in particular to a research on extracting oil by enzymatically hydrolyzing soybeans in a gas-liquid-solid three-phase magnetically stable fluidized bed. Background technique [0002] In 1983, Fullbook used enzymes to extract oil and protein from rapeseed and soybean, and achieved the desired effect. After the 1990s, the enzymatic separation of components such as oil and protein in oil has increasingly become a hot topic at home and abroad, and has been applied in oils such as coconut, rice bran, rapeseed, olive, and peanut. [0003] The aqueous enzymatic process is based on mechanical crushing, using enzymes that can degrade the cell walls of vegetable oils, or enzymes that degrade complexes such as lipoproteins and lipopolysaccharides, including cellulase, pectinase, amylase, protease etc., act on oilseeds to make oil easy to release from oilseeds, use the difference in affinity of non-oil c...

AI Technical Summary

Problems solved by technology

[0005] However, there are certain shortcomings in the extraction of oil by water enzymatic method. The enzyme cannot be regenerated and reused, resulting in a waste of enzyme resources and increased production costs.