A combined method of microalgae cultivation and waste gas denitrification
A microalgae and exhaust gas technology, applied in the joint field of microalgae cultivation and exhaust gas denitrification, can solve the problems of application limitations, inability to adapt to high-concentration ammonium salt solutions, etc., and achieve the effect of avoiding mutual influence and reliable operation
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Embodiment 1
[0070] Use BG11 medium to cultivate Chlorella (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences). Calculated by nitrogen atoms, the content of nitrogen-containing compounds in BG11 medium is 17.6mmol / L, and the temperature is controlled between 20-30°C. Initial concentration OD 680 0.5, feed compressed air and CO 2 The mixed gas for cultivation (CO 2 The concentration is 2-5v%). During the cultivation process, natural sunlight is used for cultivation, and the light intensity during the day can reach up to 60000lux. By controlling CO 2 The amount of feed is used to control the pH of the algae liquid between 7-9, and the OD value of the algae liquid is detected every day. The growth curve is shown in figure 2 Example 1-1. Harvest after 14 days of continuous culture, stop feeding CO 1 day before the end of culture 2 The pH of the algae liquid naturally rises to 9.8 at the end of the cultivation, and then, the algae mud and the alkaline algae culture...
Embodiment 2
[0074] Add KNO to BG11 medium without nitrogen source 3 Obtain the culture fluid of cultured microalgae, be used for cultivating Scenedesmus (purchased from Institute of Hydrobiology, Chinese Academy of Sciences), KNO 3 The addition amount makes in the culture solution of cultured microalgae obtained, in terms of nitrogen atoms, the content of nitrogen-containing compounds is 10mmol / L, the temperature is controlled between 20-30°C, and the initial concentration of algae species OD is 0.5. Compressed air and CO 2 The mixed gas for cultivation (CO 2 The concentration is 2-5v%). During the cultivation process, natural sunlight is used for cultivation, and the light intensity during the day can reach up to 60000lux. By controlling CO 2 The amount of feed is used to control the pH of the algae liquid between 7-9, and the OD value of the algae liquid is detected every day. The growth curve is shown in image 3 Example 2-1. Harvest after 10 days of continuous culture, stop feeding...
Embodiment 3
[0078] Add NaNO to BG11 medium without nitrogen source 3 and sodium bicarbonate to obtain a culture medium for culturing microalgae, which is used to cultivate Monostipella (purchased from the Institute of Hydrobiology, Chinese Academy of Sciences). The amount of sodium bicarbonate added makes the concentration of sodium bicarbonate in the culture medium 100mmol / L. 3 The addition amount makes in the culture solution of cultured microalgae obtained, in terms of nitrogen, the content of nitrogen-containing compound is 20mmol / L, the control temperature is between 20-30 ℃, and the initial concentration of algae is OD 680 0.3, feed compressed air and CO 2 The mixed gas for cultivation (CO 2 The concentration is 2-5v%). During the cultivation process, natural sunlight is used for cultivation, and the light intensity during the day can reach up to 60000lux. By controlling CO 2 The amount of feed is used to control the pH of the algae liquid between 7-9, and the OD value of the alg...
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