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Porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resisting and antiprotease characteristics

A technology of porcine pancreatic carboxypeptidase and pancreatic carboxypeptidase, which is applied in the biological field, can solve problems such as difficulty in achieving the effect, and achieve the effect of accelerating absorption

Active Publication Date: 2015-05-20
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to achieve the desired effect with a single enzyme preparation

Method used

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  • Porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resisting and antiprotease characteristics
  • Porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resisting and antiprotease characteristics
  • Porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resisting and antiprotease characteristics

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Experimental program
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Embodiment Construction

[0027] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0028] A porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resistant and protease-resistant properties, its extraction and activity are described as follows:

[0029] 1. The extraction process is as follows:

[0030] Take 500g of fresh pancreas from an adult domestic pig, grind it with a meat grinder, and mix it with 2 to 3 times the volume of pH 7.0, 50mmol / L phosphate buffer. Break it up in a high-speed tissue grinder, centrifuge to remove the residue, remove the supernatant and store the crude enzyme solution at 4°C.

[0031] 2. The assay process of activity is as follows:

[0032] 2.1 Folin Chromogenic Method: Take 1ml of appropriately diluted enzyme solution, preheat it in a 37°C water bath for 1min, ad...

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Abstract

The invention relates to a porcine pancreatic carboxypeptidase A1 with a transesterification function and acid-resisting and antiprotease characteristics, and the porcine pancreatic carboxypeptidase A1 is characterized in that the amino acid sequence of the porcine pancreatic carboxypeptidase A1 is shown in a sequence 1. The specific activities of the porcine pancreatic carboxypeptidase A1 (CPA1) provided by the invention, when respectively taking casein and olive oil as a substrate, are respectively 53.14 U / mg and 22.4 U / mg s, so that the porcine pancreatic carboxypeptidase A1 simultaneously has proteinase activity and transesterification activity; and the remaining enzymatic activities of the porcine pancreatic carboxypeptidase CPA1 provided by the invention, after being treated by a pH 2.0 acidic environment and trypsin, are respectively 95.65% and 78.14%, so that the porcine pancreatic carboxypeptidase A1 can resist the degradation of the strong acid environment and the trypsin, therefore, after the CPA1 is added in a feed, the nutrients absorption of the livestock and poultry can be further accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a CPA1 capable of hydrolyzing protein and decomposing oil, especially a porcine pancreatic carboxypeptidase A1 with transesterification function and acid-resistant and protease-resistant properties. Background technique [0002] Livestock and poultry cubs are immature due to their physiological development, their digestion ability is weak, and their ability to decompose and absorb nutrients in feed is low, so the growth of livestock and poultry is affected to a certain extent. Adding exogenous enzymes to the feed proved to be one of the effective solutions. Hydrolase is a general term for a class of enzymes that catalyze hydrolysis reactions, and is one of the important enzymes to improve feed digestibility and utilization. For example, adding lipase to feed can promote the digestion and decomposition of fat, releasing more medium-chain fatty acids with similar antibiotic effects; it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/48
CPCC12N9/485
Inventor 路福平张会图冯士元
Owner TIANJIN UNIV OF SCI & TECH