Application of radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines

A technology of rehmannia root and its extract, which is applied in the field of medicine, can solve problems such as the hypoglycemic effect of rehmannia rehmannia leaves, and achieve the effect of rich raw materials, easy operation, and simple preparation method

Inactive Publication Date: 2015-06-03
HENAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Rehmannia glutinosa and tuber roots are different vegetative organs of the same plant, and modern research shows that rehmannia glutinosa has a variety of chemical components. It is also reported that total glucosides of rehmannia foliage are extracted and prepared into capsules for clinical treatment of nephritis. Research Reports on the Effects of Blood Sugar

Method used

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  • Application of radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines
  • Application of radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines
  • Application of radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines

Examples

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Effect test

Embodiment 1

[0015] An application of rehmannia root extract in the preparation of hypoglycemic drugs. The rehmannia leaf extract is obtained by taking 500g of rehmannia leaf, washing away the sediment, cutting into wide strips, ventilating and drying below 40°C, and placing it in extraction equipment (such as In the extraction tank), add 10 times the weight of Rehmannia glutinosa leaves for reflux extraction twice, each time for 1h, filter, combine the filtrate (ie extract), and put the filtrate into a rotary evaporator (Shanghai Yarong Biochemical Instrument Factory, model: RE52-99), depressurize to -0.1MPa, concentrate at 55°C to a concentrated solution equivalent to 1g / ml crude drug content, add ethanol with a mass concentration of 95% to make the alcohol concentration reach 70%, and stand at 4°C for 24h , take the supernatant, concentrate under reduced pressure, and concentrate the supernatant under reduced pressure to 55°C, which is equivalent to the concentrated solution containing c...

Embodiment 2

[0017] An application of rehmannia rehmannia leaf extract in the preparation of hypoglycemic drugs. The rehmannia rehmannia leaf extract is obtained by taking 500g of rehmannia rehmannia leaf, washing away the sediment, cutting into wide shreds, ventilating and drying at a temperature below 40°C, and placing it in an extraction device. Each time add 12 times the weight of rehmannia leaves, reflux and extract twice, each time for 1h, filter, combine the filtrate, put the filtrate in a rotary evaporator, reduce the pressure to -0.1MPa, and concentrate at 55°C until the crude drug content is equivalent to 2g / ml of concentrated solution, add ethanol with a mass concentration of 95% to make the alcohol concentration reach 80%, let stand at 4°C for 24h to precipitate, take the supernatant, concentrate under reduced pressure, and concentrate the supernatant under reduced pressure to 55°C, which is equivalent to containing crude drug The concentrated solution of 2.5 mg / ml was separate...

Embodiment 3

[0019] An application of rehmannia rehmannia leaf extract in the preparation of hypoglycemic drugs. The rehmannia rehmannia leaf extract is obtained by taking 500g of rehmannia rehmannia leaf, washing away the sediment, cutting into wide shreds, ventilating and drying at a temperature below 40°C, and placing it in an extraction device. Each time add 11 times the weight of rehmannia leaves, reflux extraction twice, each time for 1.5h, filter, combine the filtrate, put the filtrate in a rotary evaporator, reduce the pressure to -0.1MPa, and concentrate at 55°C to the equivalent crude drug content 0.5g / ml concentrated solution, add ethanol with a mass concentration of 95% to make the alcohol concentration reach 60%, let it stand at 4°C for 24h, take the supernatant, concentrate under reduced pressure, and concentrate the supernatant under reduced pressure to 55°C, which is equivalent to containing The concentrated solution of crude drug 4.5mg / ml is separated and purified by H103 r...

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Abstract

The invention relates to application of a radix rehmanniae leaf extract in preparation of blood glucose-reducing medicines and can effectively solve the problem of drug use for treatment of diabetics. The preparation method comprises the following steps: adding 10-12 times of water based on weight of radix rehmanniae leaves into the radix rehmanniae leaves to extract twice, 1-1.5 hours every time; combining extraction liquids twice; concentrating the extraction liquids at a reduced pressure to a concentration liquid containing 0.5-2 g/ml of crude drugs at 55 DEG C; adding 95% by mass of ethanol into the concentration liquid, wherein the alcohol concentration reaches 60-80%; leaving to stand for 24 hours; taking a supernate; concentrating the supernate at a reduced pressure to a concentration liquid containing1-4.5g/ml of crude drugs at 55 DEG C; then separating and purifying by using an H103 resin column; then, elutingwith 10-20% by mass of ethanol as an eluent; cololecting the eluant; concentrating the eluant at a reduced pressure; drying to obtain the extract. According to the invention, the raw materials are abundant, the preparation method is simple, easy to operate and stable and reliable, and the radix rehmanniae leaf extract in any quantity can be industrially produced. The radix rehmanniae leaf extract can be effectively utilized to prepare the blood glucose-reducing medicines, so that the radix rehmanniae leaves are comprehensively developed and wastes are utilized, and a novel path of preparing the blood glucose-reducing medicines is explored.

Description

technical field [0001] The invention relates to medicine, in particular to the application of rehmannia root extract in the preparation of hypoglycemic drugs. Background technique [0002] Rehmannia glutinosa Rehmannia glutinosa The leaves of Libosch. were first published in "Diet Therapeutic Materia Medica": "(Rehmannia) leaves can be thickened", and later included in the 1988 edition of "Beijing Standards of Chinese Medicinal Materials": "Rehmannia leaves are bitter and cold, homecoming, liver, and kidney meridians, clearing away heat, Promoting blood circulation, nourishing qi and nourishing yin, nourishing kidney, used for lack of qi and fatigue, dull complexion, dry mouth and throat, deficiency of both qi and yin, externally used for malignant sores, tinea manus and pedis". Rehmannia glutinosa and tuber roots are different vegetative organs of the same plant, and modern research shows that rehmannia glutinosa has a variety of chemical components. It is also reported th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/804A61P3/10
Inventor 张振凌王娟娟刘金霞
Owner HENAN UNIV OF CHINESE MEDICINE
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