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Double-network hydrogel based on polyethylene glycol/gelatin particles and preparation method and application thereof

A technology of polyethylene glycol and double network, which is applied in the field of biomedical materials and material science, can solve the problems of not having injectable performance and not meeting the requirements, and achieve excellent mechanical strength, improve mechanical properties, and stabilize mechanical strength. Effect

Inactive Publication Date: 2020-04-21
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported double network hydrogels are often based on polymer composition and do not have injectable properties, which do not meet the material requirements for tissue filling materials and bioprinting inks

Method used

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  • Double-network hydrogel based on polyethylene glycol/gelatin particles and preparation method and application thereof
  • Double-network hydrogel based on polyethylene glycol/gelatin particles and preparation method and application thereof
  • Double-network hydrogel based on polyethylene glycol/gelatin particles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Preparation of gelatin particles

[0043] Dissolve 5g type A gelatin in 100mL deionized water solution, and keep heating to 40°C to obtain a clear and transparent gelatin solution, add hydrochloric acid dropwise to adjust the pH value of the solution to 2.5, add 240mL, 350mL acetone solution dropwise to the above gelatin solution solution and keep heating at 40°C and continuous stirring (1000rpm), the total time of dropping is 20min, add 74μL of crosslinking agent glutaraldehyde (25wt% aqueous solution) to the above nanoparticle suspension, crosslinking time 12hrs, wait for the reaction to end Finally, glycine at a concentration of 100 mM was added to the mixture to terminate the end groups of unreacted glutaraldehyde. The nanoparticle suspension was repeatedly centrifuged and resuspended in deionized water. The suspension was freeze-dried at -60°C to obtain dry powder of gelatin nanoparticles.

[0044] The gelatin particles were tested for particle size and surfa...

Embodiment 2

[0054] Embodiment 2 Regulating gelatin concentration

[0055] (1) Preparation of gelatin particles

[0056] The positively charged gelatin nanoparticles prepared in Example 1 were used.

[0057] (2) Grafting of polyethylene glycol polymeric groups

[0058] Use polyethylene glycol diacrylate with a molecular weight of 0.6kDa and 20kDa (purchased from China Sigma-Aldrich Chemical Reagent Company)

[0059] (3) The polyethylene glycol diacrylate of 0.6kDa and 20kDa and the 2-hydroxyl-4'-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator of 0.005g are dissolved respectively In 1mL of aqueous solution and 0.2g of gelatin particles repeatedly pipetted 10 times through a Luer adapter syringe to obtain a pre-polymerized colloidal gel, which has injectable and self-healing properties such as figure 1 , as shown in 2, figure 1 It shows that the prepolymerized colloidal gel has shear-thinning mechanical properties when the polymer network is not formed, and the optical image shows...

Embodiment 3

[0064] Embodiment 3 regulation and control polyethylene glycol concentration

[0065] (1) Preparation of gelatin particles

[0066] The positively charged gelatin nanoparticles prepared in Example 1 were used.

[0067] (2) Grafting of polyethylene glycol polymeric groups

[0068] Use polyethylene glycol diacrylate with a molecular weight of 0.6kDa and 20kDa (purchased from China Sigma-Aldrich Chemical Reagent Company)

[0069] (3) 0.6kDa of 0.05g, polyethylene glycol diacrylate of 20kDa and 0.005g of 2-hydroxyl-4'-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator were dissolved Injectable, self-healing pre-polymerized colloidal gel was obtained by repeatedly pipetting 0.2 g of gelatin particles in 1 mL of aqueous solution through a Luer adapter syringe for 10 times;

[0070] (4) The above-mentioned pre-polymerized colloidal gel is at 365nm, 50mw / cm 2 The double network hydrogel was obtained by cross-linking under ultraviolet light for 30s.

[0071] (5) The storage mo...

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Abstract

The invention discloses polyethylene glycol / gelatin particle dual-network hydrogel based on dual-network design and a preparation method thereof. Gelatin particles capable of dissipating energy of a network and polyethylene glycol macromolecules capable of keeping the structural integrity of the hydrogel are used, and the hydrogel material with high mechanical properties is formed on the basis ofa double-network design, when the polymer network is not formed, the reversible physical interaction between the colloidal gel materials endows the hydrogel with excellent shear thinning and self-repairing properties, and after the polymer network is formed, the mechanical properties of the hydrogel are obviously enhanced, and the hydrogel has printability and biocompatibility and has huge application potential in tissue engineering and regenerative medicine.

Description

technical field [0001] The invention belongs to the field of material science and biomedical materials, and relates to a high-strength, printable double-network hydrogel assembled from micron or nanoparticle and polyethylene glycol-based macromolecules, specifically a polyethylene glycol-based Double network hydrogel of gelatin particles and its preparation method and application. Background technique [0002] Colloidal gel is based on the bottom-up design concept, with micro- and nano-colloidal particles as the basic unit through physical or chemical "cross-linking" to form a new type of hydrogel material with fine microstructure and stable macrostructure. Micro and nano colloidal particles are assembled to form a colloidal network through physical interactions such as hydrogen bonds, electrostatics, hydrophilicity and hydrophobicity. The physical interaction between the particles makes the colloidal gel have excellent mechanical properties of injectability and self-healin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L89/00C08L71/02A61K9/06A61K47/10A61K47/42A61L27/26A61L27/50A61L27/52A61L27/56
CPCA61K9/06A61K47/10A61K47/42A61L27/26A61L27/50A61L27/507A61L27/52A61L27/56A61L2400/06A61L2430/02A61L2430/06A61L2430/22A61L2430/30C08J3/075C08J3/246C08J2371/02C08J2389/00C08J2471/02C08L89/00C08L71/02
Inventor 王华楠陈楷文
Owner DALIAN UNIV OF TECH
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