Crocodile nano-polypeptide and preparation method thereof

A kind of crocodile polypeptide, nanotechnology, applied in pharmaceutical formulations, medical preparations with inactive ingredients, antidote, etc., can solve problems such as loss of biological function, prevent the formation and deposition of amyloid, improve activity, The effect of preventing the formation of neuritic plaques

Inactive Publication Date: 2015-06-03
SHENZHEN GIANT CROCODILE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of selecting an appropriate protease to control a certain degree of hydrolysis to hydrolyze macromolecular proteins to prepare small molecular biologically active peptides is limited by the higher requirements of small molecular peptides during transportation and storage, and the ease of degradation and loss of biological functions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0054] A crocodile nano-sized polypeptide, its components and mass percentages are as follows:

[0055] Alligator Peptide 97%;

[0056] Food grade nano zinc oxide 3%;

[0057] Can achieve the best coordination complex requirements.

[0058] Crocodile polypeptide is a small molecular crocodile polypeptide obtained by hydrolyzing crocodile meat with fat tissue removed by trypsin. The molecular weight distribution range is mainly concentrated in 1000Da~1500Da.

[0059] Food-grade nano-zinc oxide is white fine particles with a particle size of 10nm to 100nm, which can be safely eaten after safety evaluation.

[0060] The preparation method of this specific embodiment one has the following steps successively:

[0061] 1) First prepare the crocodile polypeptide, followed by the following sub-steps:

[0062] 1.1) Take the crocodile meat that has been cleaned and removed from the fat tissue, and cut into pieces;

[0063] 1.2) According to volume ratio, crocodile meat: water = 1: ...

specific Embodiment approach 2

[0080] A nano-sized crocodile polypeptide, its components and mass percentages are the same as in Embodiment 1, and the preparation method is basically the same as Embodiment 1, the difference is that:

[0081] 1) The complexing time is 40min;

[0082] 1.2) The volume ratio is crocodile meat: water = 1:2.5 (m 3 / m 3 );

[0083] 1.3) Lower the temperature to 55°C and maintain it at a constant temperature, and adjust the pH to 7.8;

[0084] 1.4) The mass ratio is crocodile meat: food grade trypsin powder=1:0.40%, and the enzymolysis time is 5h;

[0085] 5) The atomic force microscope produced by Swiss Nanosurf company was used to prepare the sample by centrifugal sedimentation method.

[0086] The degree of hydrolysis of the protein in the crocodile polypeptide enzymatic hydrolyzate obtained in this specific embodiment is: more than 60%; the compounding rate of the crocodile polypeptide-nanometer zinc oxide complex reaction is: 90%; the yield of the crocodile nano-sized poly...

specific Embodiment approach 3

[0087] A nano-sized crocodile polypeptide, its components and mass percentages are the same as in Embodiment 1, and the preparation method is basically the same as Embodiment 1, the difference is that:

[0088] 1) The complexing time is 30min;

[0089] 1.2) The volume ratio is crocodile meat: water = 1:2.0 (m 3 / m 3 );

[0090] 1.3) Lower the temperature to 55°C and maintain it at a constant temperature, and adjust the pH to 7.5;

[0091] 1.4) The mass ratio is crocodile meat: food grade trypsin powder=1:0.38%, and the enzymolysis time is 5h;

[0092] The degree of hydrolysis of protein in the crocodile polypeptide enzymatic hydrolyzate obtained in this specific embodiment is 53%; the complexation rate of crocodile polypeptide-nano-zinc oxide complex reaction is 91%; the yield of crocodile nano-sized polypeptide is 10%.

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Abstract

The invention discloses a crocodile nano-polypeptide which comprises the following components in percentage by weight: 96-98% of crocodile polypeptide and the balance of food grade nano zinc oxide. The invention further discloses a preparation method of the crocodile nano-polypeptide. The preparation method sequentially comprises the following steps: 1) carrying out coordinated complexation on crocodile polypeptide and food grade nano zinc oxide; 2) cooling; 3) washing, settling and carrying out centrifugal separation; 4) freezing and drying; and 5) carrying out quality control analysis. The crocodile nano-polypeptide disclosed by the invention is relatively stable in structure and bioactivity, can be used for improving the biological value and nutritional value of proteins and zinc and has the functions of resisting generation and deposition of amyloid proteins and preventing formation of neuritic plaques so as to realize the function of preventing or delaying the progress of course of disease of senile dementia. The crocodile nano-polypeptide further has the functions of resisting oxidization, preventing fatigue, enhancing the immunity, improving the activity of brain cells and enhancing the memory and ability of thinking and is very applicable to developing products for preventing chronic diseases such as hypertension, cardiovascular and cerebrovascular diseases and senile dementia as well as novel raw materials for nutritional therapy.

Description

technical field [0001] The invention relates to biological extracts, in particular to a crocodile nanometerized polypeptide and a preparation method thereof. Background technique [0002] Crocodiles are among the earliest and most primitive animals ever discovered. Modern studies have shown that crocodile meat extract has the functions of enhancing immunity, enhancing learning and memory, improving hypoxia tolerance and anti-lipid oxidation. Most of the existing crocodile-derived biological extracts have not been deeply explored in terms of their principles and molecular mechanisms, and there is a waste of biological functional substances. [0003] Bioactive peptides not only have the characteristics of easy digestion and absorption and high safety, but also have special physiological regulation functions, including lowering blood lipids, delaying aging, enhancing memory, enhancing immunity, improving sleep and inhibiting tumors. However, the method of selecting an appropr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61K47/48A61P25/28A61P39/06A61P39/00A61P37/04A61P25/00A61P9/12A61P9/10A61P9/00A61P3/02
Inventor 郑文岭张晓松莫丽春
Owner SHENZHEN GIANT CROCODILE BIOTECH CO LTD
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