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Method for enriching and purifying α-arbutin from blueberry leaves

A technology of arbutin and blueberry leaves, which is applied in the field of preparing α-arbutin from wild blueberry leaves in Daxing’an Mountains, which can solve the problems of high price of α-glucosidase, high cost of α-arbutin, and difficulty in practical promotion.

Inactive Publication Date: 2018-04-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main method of producing α-arbutin at home and abroad is the synthesis method, which uses α-glucosidase as a catalyst to catalyze the synthesis of hydroquinone and glucose into α-arbutin. Since α-glucosidase is expensive, the α-arbutin produced - The cost of arbutin is very high, and the yield of α-arbutin prepared by the above method is not high, which brings great difficulties to the actual promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Crush the blueberry leaves into blueberry leaf powder with a mesh size of 20-40 mesh; mix the blueberry leaf powder with a solvent evenly and then extract it with a high-voltage pulse electric field extractor. The ratio of extraction material to liquid is 1:5 (w / v). The solvent is 50% alcohol, the extraction temperature is 42°C, the extraction time is 70min, and the extraction is performed 3 times, the electric field strength is 15kv / cm, the pulse number is 17, and the extract is collected; the collected extract is concentrated under vacuum pressure, The vacuum degree is -0.08Mpa, the concentration temperature is at 50°C, and the solvent is recovered to obtain the concentrate; add 50% deionized water of the raw material weight to the concentrate to dilute the concentrate, and then use a microfiltration membrane for pretreatment before using an ultra- The material of the microfiltration membrane and the ultrafiltration membrane is vinylidene fluoride (PVDF). 30000Dal, fe...

Embodiment 2

[0024] Crush the blueberry leaves into blueberry leaf powder with a mesh size of 20-40 mesh; mix the blueberry leaf powder with a solvent evenly and then extract it with a high-voltage pulse electric field extractor. The ratio of extraction material to liquid is 1:7 (w / v). The solvent is 60% alcohol, the extraction temperature is 44°C, the extraction time is 80min, the extraction is performed twice, the electric field strength is 13kv / cm, the pulse number is 19, and the extract is collected; the collected extract is concentrated under vacuum pressure, The vacuum degree is -0.07Mpa, the concentration temperature is at 55°C, the solvent is recovered, and the concentrate is obtained; the concentrate is diluted by adding 40% raw material weight deionized water to the concentrate, and then pretreated with a microfiltration membrane The material of the microfiltration membrane and the ultrafiltration membrane is vinylidene fluoride (PVDF). 80000 Dal, the feed liquid temperature is 3...

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Abstract

The invention belongs to the field of natural organic chemistry and relates to an extraction and separation method of alpha-arbutin from blueberry leaves. The method comprises the following steps: firstly extracting alpha-arbutin by using high voltage pulse electric field extraction equipment; then primarily purifying the alpha-arbutin by using membrane equipment and macroporous resin; then separating and purifying the alpha-arbutin by virtue of a simulated moving bed chromatographic separation technology to obtain a high purity alpha-arbutin product. The method provided by the invention has the advantages that 1, an extraction solvent can enter into the inner parts of plant cells by virtue of a high voltage electric field technology, so that the alpha-arbutin is efficiently extracted; 2, the alpha-arbutin is separated and purified by virtue of the simulated moving bed chromatographic separation technology, so that the alpha-arbutin obtained is high in purity. The prepared alpha-arbutin can be widely applied to the field of health foods, cosmetics and the like.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for preparing α-arbutin from Daxinganling wild blueberry leaves as raw materials. Background technique [0002] α-arbutin (α-arbutin) is a natural active substance, which can effectively inhibit the biological tyrosinase activity in the skin, block the formation of melanin, accelerate the decomposition and excretion of melanin, thereby reducing skin pigmentation, It is the main active substance in whitening cosmetics at home and abroad to remove stains and freckles. In recent years, with the development of the domestic cosmetics industry, in order to comply with the trend of "returning to nature" in skin care cosmetics, the "green whitening" of natural active substances has become increasingly popular, and arbutin, as a non-toxic and harmless natural active substance with obvious whitening effect Material, the market demand is expanding day by day. [0003] At pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 苏学辉姚德坤
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD