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Micro RNA for accelerating tissue-engineered bone vascularization and application thereof

A tissue-engineered bone and vascularization technology, applied in the field of genetic engineering, can solve problems such as hindering the migration of vascular endothelial cells, and achieve the effect of overcoming the instability of blood vessels and high biological safety

Inactive Publication Date: 2015-06-10
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

Some scholars have adopted a controllable slow-release system to release all the above growth factors into the graft to produce induction simultaneously, but this slow-release system hinders the host-derived vascular endothelium that plays a major role in angiogenesis in the graft area. Migration of cells into the graft and therefore does not produce the desired effect of promoting vascularization

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  • Micro RNA for accelerating tissue-engineered bone vascularization and application thereof
  • Micro RNA for accelerating tissue-engineered bone vascularization and application thereof
  • Micro RNA for accelerating tissue-engineered bone vascularization and application thereof

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Embodiment Construction

[0027] MicroRNA (microRNA) is a kind of eukaryotic endogenous small molecule single-stranded RNA, usually 18-25 bp in length, which can cause the degradation of target mRNA or inhibit its translation through specific base pairing with target mRNA, thereby affecting Post-transcriptional expression regulation of genes is an important molecular tool in RNA interference. In recent years, hundreds of miRNAs have been discovered in animal cells and plant tissues. These small molecular regulatory RNAs are matured by cutting out the 60-200bp precursor with a hairpin structure.

[0028] In animal cells, the initial product of microRNA gene transcription (pri-miRMA) is quickly processed by ribonuclease III Drosha (RNase III Drosha) into a microRNA precursor (pre-miRNA), and then transported from the nucleus to the cytoplasm, where it passes through another RNase III Dicer Recognizes spliced ​​mature microRNA. These mature microRNAs and other proteins together form the RISC complex (RNA...

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Abstract

The invention discloses micro RNA for accelerating tissue-engineered bone vascularization and application thereof and belongs to the technical field of gene engineering. The micro RNA is miR-26a, and the sequence is shown as SEQ. ID. NO. 1. The micro RNA for accelerating the tissue-engineered bone vascularization uses a mice skull defect regeneration model, preferential expression of the miR-26a is discovered in new bone, after transferring the miR-26a mimics and inhibitor into cell in vitro to realize the miR-26a expression increasing and decreasing, observing the regulation effect of the miR-26a for growth factors related to the angiogenesis in vitro in three osteogenesis related cell models, and using an in vivo and in situ bone defect and ectopic bone regeneration model to further verify the vascularization accelerating effect of the miR-26a in vivo.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a microRNA that promotes vascularization of tissue engineered bone and its application. Background technique [0002] The high incidence of large bone defects caused by bone trauma and bone tumors has forced orthopedic doctors and workers in related disciplines to work hard to find suitable and effective grafts for repair. Tissue-engineered bone has increasingly become a research hotspot due to its multiple advantages such as good biological safety, plasticity, and not being restricted by donor sources. Tissue engineered bone construction is mainly the process of compounding seed cells and scaffold materials. However, the current clinical application of tissue engineered bone is facing many problems, among which the biggest obstacle is that the grafted bone cannot obtain sufficient blood supply in a short period of time, resulting in the death of most of ...

Claims

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Application Information

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IPC IPC(8): C12N15/113A61K48/00A61K31/7105A61P9/00
Inventor 陈吉华李岩
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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