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Preparation of different active positions of artemisia sacrorum extract and application of artemisia sacrorum extract serving as acaricidal active substance

A technology of active parts, Artemisia annua, applied in the field of botanical pesticides, can solve problems such as no research reports on the function of Artemisia annua in preventing and controlling diseases and insect pests, and achieve the effects of being easy to cultivate and plant in a large area, improving stress resistance, and having a simple preparation process.

Active Publication Date: 2015-06-17
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the function of using Artemisia chinensis to control diseases and insect pests.

Method used

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  • Preparation of different active positions of artemisia sacrorum extract and application of artemisia sacrorum extract serving as acaricidal active substance
  • Preparation of different active positions of artemisia sacrorum extract and application of artemisia sacrorum extract serving as acaricidal active substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Preparation of Artemisia albicans extract: collect fresh aerial parts of Artemisia albicans plants at maturity (August), dry in the shade, and pulverize. Add 20 times the volume of 80% ethanol aqueous solution, ultrasonically extract for 30 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain the extract, and dry it in a ventilated and dark place to obtain the Artemisia alba extract.

[0035]2. Preparation of different active parts of Artemisia basilicum: Dissolve the prepared Artemisia baicalensis extract with 200 times the volume of distilled water, add petroleum ether 3 times the volume of the aqueous solution to the aqueous solution, vibrate vigorously, leave to stand for stratification, and the supernatant It is the petroleum ether layer; repeat the extraction 3 times, combine the petroleum ether layers, and concentrate under reduced pressure to obtain the active part of the petroleum ether phase of Bail...

Embodiment 2

[0038] 1. The preparation of the Artemisia alba extract: the same as in Example 1.

[0039] 2. Preparation of different active parts of Artemisia basilicum: Dissolve the prepared Artemisia baicalensis extract with 1000 times the volume of distilled water, add petroleum ether 5 times the volume of the aqueous solution to the aqueous solution, vibrate vigorously, leave to stand for stratification, and the supernatant It is the petroleum ether layer; repeat the extraction 3 times, combine the petroleum ether layers, and concentrate under reduced pressure to obtain the active part of the petroleum ether phase of Bailian Artemisia. Add 5 times the volume of ethyl acetate to the remaining aqueous solution after petroleum ether extraction, vibrate vigorously, let stand to separate layers, the supernatant is the ethyl acetate layer, repeat the extraction 3 times, combine the ethyl acetate layers, concentrate under reduced pressure, and prepare The active part of the ethyl acetate phas...

Embodiment 3

[0042] 1. Preparation of Artemisia albicans extract: collect fresh aerial parts of Artemisia albicans plants at maturity (August), dry in the shade, and pulverize. Add 20 times the volume of 80% ethanol aqueous solution, reflux extraction for 90 minutes, and repeat the extraction 3 times. Concentrate the extraction solvent under reduced pressure to obtain the extract, and dry it in a ventilated and dark place to obtain the Artemisia alba extract.

[0043] 2. Preparation of different active parts of Artemisia basilicum: Dissolve the prepared Artemisia baicalensis extract with 1500 times the volume of distilled water, add petroleum ether 8 times the volume of the aqueous solution to the aqueous solution, vibrate vigorously, leave to stand for stratification, and the supernatant It is the petroleum ether layer; repeat the extraction 3 times, combine the petroleum ether layers, and concentrate under reduced pressure to obtain the active part of the petroleum ether phase of Bailian...

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Abstract

The invention discloses a preparation of different active positions of an artemisia sacrorum extract. The preparation method comprises the following steps: drying overground parts of fresh artemisia sacrorum plants at a maturation stage in shade, crushing, carrying out alcohol extracting, carrying out vacuum concentrating and drying to obtain the artemisia sacrorum extract; dissolving the artemisia sacrorum extract in distilled water, sequentially extracting by using petroleum ether, ethyl acetate and normal butanol to obtain four different active parts of petroleum ether, ethyl acetate, normal butanol and aqueous phase. An acaricide prepared by using the artemisia sacrorum as a plant source has very obvious contact toxicity on agricultural mites, has a growth-promoting effect on crops, improves the productivity and stress resistance of the crops in a mite killing process and promote the increase of yield and income in agricultural production. As a safe, non-toxic and environment-friendly plant source pesticide, the artemisia sacrorum extract is environmentally friendly, is safe to natural enemies of pests and people and livestock, and has great significance of establishing novel green ecological agriculture.

Description

technical field [0001] The invention belongs to the technical field of botanical pesticides, and relates to the preparation of an acaricidal active substance, in particular to the preparation of different active parts of Artemisia basilicum; at the same time, the present invention also relates to the acaricidal activity of different active parts of Artemisia baicalensis and the method for preparing an acaricide application. Background technique [0002] Mites belong to the class Arachnida and the subclass Acari. There are about 300,000 to 500,000 species in the world, and they are the second largest biological group in the animal kingdom after Insecta. According to its economic significance, it can be divided into two categories: medical and animal husbandry acarids and agricultural pest mites. Agricultural pest mites destroy the normal physiological functions of plants, causing leaf drop, bud drop, and fruit drop, which can cause yield reduction in mild cases, or the death...

Claims

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Application Information

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IPC IPC(8): A01N65/12A01P7/02A01P21/00
Inventor 刘权李晓蓓贺原花
Owner LANZHOU UNIVERSITY
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