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Indel Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application

A molecular marker, corn technology, applied in the field of molecular genetics, can solve the problem of time-consuming and laborious QTL positioning, and achieve the effect of improving low phosphorus tolerance and high application value

Inactive Publication Date: 2017-08-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, QTL mapping is time-consuming and labor-intensive, and most of the QTL intervals are wide. At present, there are no reports on the development and utilization of molecular markers for the main effect loci of low phosphorus tolerance in maize germplasm improvement and molecular assisted breeding.

Method used

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  • Indel Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application
  • Indel Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application
  • Indel Molecular Marker of Maize Low Phosphorus Response Gene zmarf31 and Its Application

Examples

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Effect test

Embodiment 1

[0034] Example 1 The acquisition of an InDel molecular marker that is significantly correlated with the number of root tips in maize low phosphorus response gene ZmARF31 and the determination of its detection primers

[0035] In the present invention, an InDel molecular marker that is significantly related to the low phosphorus response gene ZmARF31 of maize and the number of root tips is obtained by the following method:

[0036] 1) A total of 331 maize inbred lines from China, the United States and Mexico were collected, and the related populations used for mapping were constructed. The population has rich genetic diversity, including 131 temperate maize inbred lines and 200 tropical / subtropical maize inbred lines.

[0037]2) Identification of root phenotypes at the seedling stage of related populations. In 2010, Sichuan Agricultural University Duoying Farm Base and in 2012, Sichuan Agricultural University Ya'an Teaching and Experimental Greenhouse, 331 maize inbred lines w...

Embodiment 2

[0043] Embodiment 2 The application test on the InDel mark corn root tip number of the present invention

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Abstract

The invention provides an InDel molecular marker in a low-phosphorus response gene ZmARF31 of corn. The InDel marker, i.e., an SNP marker, is located at the position 148637125 bp of chromosome 10, and an allelic gene is insertion / deletion of 38 bp, wherein the sequence of the 38 bp is shown in SEQ ID No.4; the flanking sequence of the allelic gene is shown in SEQ ID No.1; and the SNP marker is obtained through amplification of primers with nucleotide sequences as shown in SEQ ID No.2 and 3. The InDel molecular marker is significantly correlated to the numbers of root tips of corn at a normal phosphorus level and under low phosphorus stress; and the root tip numbers of inbred-line corn with a site genotype of 38 bp insertion are higher than the root tip numbers of inbred-line corn with a site genotype of 38 bp deletion at the normal phosphorus level and under low phosphorus stress. The InDel molecular marker of the invention can be used for assistant breeding of the corn and accelerates creative preparation of a phosphorus highly-efficient metamaterial of the corn and selective breeding of novel varieties of the corn.

Description

technical field [0001] The present invention relates to the field of molecular genetics, in particular to molecular markers related to corn low-phosphorus tolerance traits, specifically, to a SNP marker significantly associated with total dry weight in the ZmARF31 gene on chromosome 10 of corn, and the method of amplifying it Primer pairs and the use of the molecular marker. Background technique [0002] Corn is a multivariate crop that integrates grain, economics, and feed. It is also an important industrial raw material and energy crop, and is widely distributed around the world. At the end of the 20th century, with the rapid growth of the global demand for corn, its planting area gradually surpassed that of rice and wheat, and became the food crop with the largest total output in the world. Maize is expected to account for 50 percent of the increase in food production by 2050. Phosphorus is one of the three major nutritional elements necessary for plant growth and devel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/82C12Q1/68A01H5/00
Inventor 卢艳丽刘玲吴锋锴王琦熊静徐洁高世斌唐祈林兰海吴元奇
Owner SICHUAN AGRI UNIV