Procalcitonin detection kit

A technology for detecting kits and procalcitonin, which is applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as inability to quantitatively detect, and achieve the effects of improving detection sensitivity, reducing costs, and reducing dosage

Inactive Publication Date: 2015-06-17
上海国兴医疗器械有限公司
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  • Abstract
  • Description
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Problems solved by technology

In addition, there is a colloidal gold reagent for qualitative detection of PCT on the market, which cannot be quantitatively d

Method used

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Embodiment

[0014] Procalcitonin detection kit, including magnetic particle separation reagent, PCT detection antibody, enzyme conjugate, luminescent substrate, PCT standard, enzyme diluent for diluting the enzyme conjugate; in the magnetic particle separation reagent The magnetic particle-coupled protein molecule is mouse anti-human immunoglobulin G; the PCT detection antibody is anti-mouse IgG antibody; the enzyme conjugate is horseradish peroxidase-labeled mouse anti-human IgG; the luminescent substrate It is an HRP luminescent substrate; the HRP luminescent substrate includes A solution and B solution, and the A solution and B solution of the HRP luminescent substrate are mixed 1:1 in practice.

[0015] The A solution of the HRP luminescent substrate includes a light agent luminol or a luminol derivative, an enhancer and a buffer, and the B solution of the HRP luminescent substrate includes sodium perborate and hydrogen peroxide.

[0016] The size of the magnetic particles in the magn...

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PUM

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Abstract

Disclosed is a procalcitonin (PCT) detection kit, which comprises a magnetic particle separation reagent, a PCT detection antibody, an enzyme conjugate, a photogenic substrate, a PCT standard substance, and an enzyme diluent for diluting the enzyme conjugate. The magnetic particle separation reagent contains a magnetic particle coupled protein molecule, which is anti-rat human immunoglobulin G; the PCT detection antibody is an anti-rat IgG antibody; and the enzyme conjugate anti-rat human IgG labeled by horse radish peroxidase. According to the invention, an indirect coating mode that anti-rat human immunoglobulin G is coupled with magnetic particles, and a PCT monoclonal antibody combines with the anti-rat human immunoglobulin G is adopted. Through such mode, the dosage of the PCT monoclonal antibody is lowered, so that cost is reduced. The detection sensitivity is obviously improved by adding the concentration of anti-rat human immunoglobulin G of coupled magnetic particles.

Description

technical field [0001] The invention relates to a detection technology for procalcitonin, in particular to a procalcitonin detection kit. Background technique [0002] Serum calcitonin (CT) is a polypeptide hormone that was first extracted from thyroid tumor cell culture medium, and thus becomes a serological marker of this tumor. Procalcitonin (PCT), the precursor of CT, is a glycoprotein composed of 116 amino acids discovered by German scientists in 1992. Its half-life in the human body is about 20-24 hours, and it has good stability; The content in normal human serum is extremely low, and in addition to thyroid trauma or tumor, it is significantly elevated in the serum of patients with systemic inflammatory response syndrome (SIRS), sepsis, acute and chronic pneumonia, acute pancreatitis, active hepatitis, trauma, etc. A very sensitive and specific serological marker. However, it remains low during viral infection and tumor surgery trauma, and PCT can increase in the ea...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/54326G01N33/6854G01N2446/90
Inventor 不公告发明人
Owner 上海国兴医疗器械有限公司
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