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Application of α-ketoglutarate in improving bacterial susceptibility to antibiotics

A technology of ketoglutaric acid and antibiotics is applied in the field of medicine to achieve the effects of overcoming drug resistance, overcoming bacterial drug resistance and improving sensitivity

Active Publication Date: 2018-07-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on whether α-ketoglutarate can improve antibiotics to clear pathogenic bacteria

Method used

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  • Application of α-ketoglutarate in improving bacterial susceptibility to antibiotics
  • Application of α-ketoglutarate in improving bacterial susceptibility to antibiotics
  • Application of α-ketoglutarate in improving bacterial susceptibility to antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 α-ketoglutarate can increase the sensitivity of bacteria to kanamycin in a concentration-dependent manner

[0023] S1. Preparation of bacterial samples: Pick a single colony of Escherichia coli K12 BW25113 or Edwardsiella tarda EIB202 and culture them in 100 mL of LB or TSB liquid medium at 37°C or 30°C at 200 rpm until saturated. Centrifuge at 8000rpm at room temperature for 2min, collect the bacteria, wash the bacteria three times with sterile saline, and finally adjust the washed bacteria to OD with 1×M9 medium 600 =0.2, draw 5mL of the bacterial solution and distribute it in test tubes for later use.

[0024] S2. Under the premise of 40μg / mL kanamycin, add α-ketoglutaric acid (sterilized by filtration) to make the final concentration of 0, 1.25, 2.5, 5, 10 and 20mM (BW25113) or 0, 0.3, 0.6, 1.2, 2.5 and 5mM (EIB202). Cultivate at 37°C or 30°C, 200rpm for 6 hours, detect the number of viable bacteria with a plate, and calculate the survival rate of differ...

Embodiment 2

[0026] Example 2 α-ketoglutarate can increase the sensitivity of bacteria to various antibiotics

[0027] BW25113 and EIB202 cell samples were prepared according to the method in S1 of Example 1, and different concentrations of different antibiotics were added under the premise of adding α-ketoglutarate at a final concentration of 2.5 mM. For BW25113, add 5 μg / mL gentamicin, 0.5 μg / mL kanamycin, 20 μg / mL streptomycin and 0.5 μg / mL ceftazidime; for EIB202, add 2 μg / mL tetracycline, Chloramphenicol at 200 μg / mL, rifampicin at 12.5 μg / mL, and gentamicin at 16 μg / mL. After incubation at 37°C (BW25113) or 30°C (EIB202) at 200rpm for 6 hours, the number of viable bacteria was detected on a plate, and the survival rate of bacteria with different concentrations of antibiotics was calculated at the concentration of 2.5mM α-ketoglutarate. result( figure 2 ) found that α-ketoglutarate can increase the sensitivity of bacteria to a variety of antibiotics, and the improvement factor is b...

Embodiment 3

[0028] Example 3 α-ketoglutarate can increase the sensitivity of Gram-positive and Gram-negative bacteria to antibiotics

[0029] Select B-hemolytic Streptococcus, Staphylococcus aureus (Gram-positive bacteria) and Pseudomonas aeruginosa (Gram-negative bacteria) to prepare bacterial samples according to the S1 method of Example 1, and add the final concentration to each tube Under the premise of 2.5mM α-ketoglutarate, 8μg / mL gentamicin and 400μg / mL kanamycin (Streptococcus B), and 400μg / mL kanamycin were added and not added, respectively. (Pseudomonas aeruginosa) and 60μg / mL ampicillin (Staphylococcus aureus), after incubation at 37°C and 200rpm for 6h, the number of viable bacteria was detected on the plate, and calculated at 2.5mM α-ketoglutarate , adding different kinds of antibiotics to the survival rate of different kinds of bacteria. by the result ( image 3 ) It can be seen that α-ketoglutaric acid can increase the sensitivity of various bacteria to various antibiotic...

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Abstract

The invention belongs to the technical field of medicine, and specifically discloses the application of α-ketoglutarate in improving the susceptibility of bacteria to antibiotics. The present invention finds through research that α-ketoglutarate can improve the sensitivity of bacteria to antibiotics, thereby overcoming the problem of bacterial drug resistance. Therefore, the combined use of α-ketoglutarate and antibiotics can significantly improve the bactericidal effect of antibiotics, and compared with the existing antibacterial drugs that only use antibiotics, it has better effects and higher safety and operability.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of alpha-ketoglutaric acid in improving the susceptibility of bacteria to antibiotics. Background technique [0002] Pathogenic bacteria seriously endanger human health and the sustainable development of aquaculture. Although the use of antibiotics can be effective in prevention and control, the overuse and misuse of antibiotics can lead to bacterial resistance. After the bacteria are resistant, they develop resistance to the originally effective antibiotics, making it difficult to control the infection. Therefore, it is very important to adopt new methods to control the infection of bacteria, especially drug-resistant bacteria. [0003] One technical method is to increase the susceptibility of drug-resistant bacteria to antibiotics, making the originally ineffective or ineffective antibiotics effective and killing drug-resistant bacteria. Therefore, it is ver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/194A61P31/04
Inventor 彭宣宪李惠苏玉斌宗云峰
Owner SUN YAT SEN UNIV