Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for regulating biological enzymes and its degradation of environmental hormone carbaryl by ionic liquid, and a method for determining the degradation rate

A technology of ionic liquids and environmental hormones, applied in biochemical equipment and methods, enzyme stabilization, chemical instruments and methods, etc., can solve the problems of expensive membrane modules, high cost of chemical methods, incomplete treatment, etc., and achieve fast degradation rate , high catalytic efficiency, and the effect of improving the effective utilization rate

Inactive Publication Date: 2018-04-27
SHAANXI NORMAL UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the removal rate of membrane separation technology is relatively good, but the treatment process is not only related to the properties of the target object, but also related to the properties of the membrane and the composition of the solution, and the price of the membrane module is relatively expensive, and there are often clogging and The state of swelling, so it is difficult to widely apply membrane separation technology in wastewater treatment
The physical adsorption method has relatively high cost, incomplete treatment and many uncertainties in the treatment process.
Although using chemical oxidation method to degrade carbaryl can degrade organic matter more efficiently, non-selectively and relatively completely, new impurities will inevitably be introduced in the process of treatment, which will cause a burden to the environment; and the cost of chemical method is relatively high. There are still some difficulties in the whole process of industrial wastewater treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for regulating biological enzymes and its degradation of environmental hormone carbaryl by ionic liquid, and a method for determining the degradation rate
  • A method for regulating biological enzymes and its degradation of environmental hormone carbaryl by ionic liquid, and a method for determining the degradation rate
  • A method for regulating biological enzymes and its degradation of environmental hormone carbaryl by ionic liquid, and a method for determining the degradation rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Take the pH value as 5.8 and the concentration as 0.1mol L -1 KH 2 PO 4 -K 2 HPO 4 solution, adjusted to a pH 2.75 phosphate buffer solution with hydrochloric acid.

[0034] (2) According to carbaryl and H 2 o 2 The molar ratio of the substance is 1: (9~12), and the initial concentration of 500 μL is 100 μmol L -1 Carbaryl solution, 5 μL initial concentration is 4.8 μmol L -1 Chloroperoxidase solution, 36 μL with an initial concentration of 10mol L -1 Tetramethylammonium bromide solution, and 45-60 μL with an initial concentration of 10 mmol L -1 H 2 o 2 The solution was added to the phosphate buffer solution respectively, and the volume was adjusted to 3mL by the phosphate buffer solution, and mixed evenly to form a reaction system. In the reaction system, the concentration of each substance was as follows: the concentration of chloroperoxidase was 0.008 μmol L -1 , the concentration of tetramethylammonium bromide is 0.12mol L -1 , H 2 o 2 The concent...

Embodiment 2

[0047] (1) Take the pH value as 5.8 and the concentration as 0.1mol L -1 KH 2 PO 4 -K 2 HPO 4 solution, adjusted to a pH value of 2 phosphate buffer solution with hydrochloric acid.

[0048] (2) According to carbaryl and H 2 o 2 The molar ratio of the substance is 1:6, take 500 μL of the initial concentration of 100 μmol L -1 Carbaryl solution, 5 μL with an initial concentration of 30 μmol L -1 Chloroperoxidase solution, 60 μL with an initial concentration of 10mol L -1 Tetramethylammonium bromide solution, and 30 μL with an initial concentration of 10 mmol L -1 H 2 o 2 The solution was added to phosphate buffer solution respectively, and the volume was adjusted to 3mL by phosphate buffer solution, and mixed evenly to form a reaction system. In the reaction system, the concentration of each substance was as follows: the concentration of chloroperoxidase was 0.05 μmol L -1 , the concentration of tetramethylammonium bromide is 0.20mol L -1 , H 2 o 2 The concentrati...

Embodiment 3

[0052] (1) Take the pH value as 5.8 and the concentration as 0.1mol L -1 KH 2 PO 4 -K 2 HPO 4 solution, adjusted with hydrochloric acid to a phosphate buffered solution with a pH of 2.5.

[0053] (2) According to carbaryl and H 2 o 2 The molar ratio of the substance is 1:3, take 500 μL of the initial concentration of 100 μmol L -1 Carbaryl solution, 5 μL initial concentration is 6 μmol L -1 Chloroperoxidase solution, 18 μL with an initial concentration of 10mol L -1 Tetraethylammonium bromide solution, and 15 μL for an initial concentration of 10 mmol L -1 H 2 o 2 The solution was added to the phosphate buffer solution respectively, and the volume was adjusted to 3mL by the phosphate buffer solution, and mixed evenly to form a reaction system. In the reaction system, the concentration of each substance was as follows: the concentration of chloroperoxidase was 0.01 μmol L -1 , the concentration of tetraethylammonium bromide is 0.06mol L -1 , H 2 o 2 The concentrat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to an ionic liquid regulation-control bio-enzyme, a method for degrading environmental hormone carbaryl by the same, and a method for determining the degradation rate. The ionic liquid regulation-control bio-enzyme comprises the mixed solution formed by adding chloroperoxidase, quaternary ammonium salt ionic liquids and H2O2 in a phosphate buffer solution with a pH value of 2-5, wherein in the mixed solution, the concentration of chloroperoxidase is 0.005-0.05mu mol.L<-1>, the concentration of ammonium salt ionic liquids is 0.06-0.20mol.L<-1>, and the concentration of H2O2 is 0.05-0.5mmol.L<-1>. According to the ionic liquid regulation-control bio-enzyme, the method for degrading environmental hormone carbaryl by the same, and the method for determining the degradation rate disclosed by the invention, the chloroperoxidase is used as a biocatalyst, the ammonium salt ionic liquids are used as regulation-control factors, the ammonium salt ionic liquids have a cosolvent effect on the chloroperoxidase, and can be used for improving the stability of the chloroperoxidase, increasing the enzyme catalysis speed and increasing the effective utilization rate of the chloroperoxidase; the ionic liquid regulation-control bio-enzyme is capable of effectively improving the solubility of carbaryl and increasing the degradation rate in case of being applied to degradation for carbaryl.

Description

Technical field: [0001] The invention relates to the field of biodegradation, in particular to a method for regulating biological enzymes by an ionic liquid, a method for degrading the environmental hormone carbaryl, and a method for measuring the degradation rate. Background technique: [0002] In the 1950s, carbamate pesticides represented by carbaryl (CBL) entered the market and became the second main force after organophosphorus pesticides. Carbaryl, also known as carbaryl or carbaryl, has good residual effects and systemic properties, and has contact and stomach poisoning effects on pests. It is a broad-spectrum insecticide and is widely used in fruit trees. , vegetables, grain, medicinal materials, tea and other crops. However, as it is widely used, its toxic effects are also increasingly exposed, especially in recent years, it has been found that it has a disturbing effect on the human endocrine system, and belongs to environmental hormones (EDS), which can damage th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96C12N9/08A62D3/02C02F3/34G01N30/02A62D101/04A62D101/28
CPCA62D3/02A62D2101/04A62D2101/28C02F3/342C02F2101/306C02F2101/38C12N9/0065C12N9/96C12Y111/01G01N30/02G01N2030/027
Inventor 蒋育澄王圣洁胡满成李淑妮翟全国
Owner SHAANXI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com