Sugarcane juice functional beverage and preparation method thereof
A technology of sugarcane juice and seeds, applied in the field of bioengineering, can solve the problems of poor taste and low health care effect of sugarcane juice beverages, and achieve the effects of unique taste, improved health care function, and desalinated bitterness
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Embodiment 1
[0023] 1. Fermented seed culture of Ganoderma lucidum
[0024] Potatoes 180-220 g, add 1L distilled water, boil for about 30 minutes, filter the juice, add glucose 20g, peptone 2-3g, KH 2 PO 4 1-1.5 g, MgSO 4 0.5g, distilled water to 1L, put into 500ml Erlenmeyer flasks (containing glass beads), 200ml per bottle, autoclave at 121°C for 25-30 minutes, after the temperature drops below 30°C, connect Put 4-6 pieces of seed pieces at the edge of the colony of Ganoderma lucidum, place them on a shaker, and cultivate them with shaking at 22-26°C, 150-180 r / min, for 5-7 days. Shake flask culture without glass beads was used as a control. The biomass, uniformity and mycelium activity of the seed solution obtained by the two methods were compared.
[0025]
[0026] Two different seed liquids cultivated for 5 days were added to the sugarcane juice medium with an inoculation amount of 5% (V / V). After inoculation, they were placed on a shaker at 25°C, 150 r / min, and shaken for 10...
Embodiment 2
[0034] A preparation method for sugarcane juice fermentation protoplasm, comprising the steps of:
[0035] 1. Selection of fermentation medium
[0036] Sugarcane juice medium: Wash the sugarcane and squeeze the juice, adjust the sugar content to 80-120g / L with distilled water, and the pH is natural.
[0037] PDB medium (g / L): Potato 200, add distilled water, boil for about 30 minutes, filter to get juice, add glucose 20, KH 2 PO 4 1, MgSO 4 0.5, pH natural.
[0038] Ordinary fermentation medium (g / L): glucose 50, corn flour 10, peptone 3, soluble starch 10, MgSO 4 0.5, KH 2 PO 4 1. pH is natural.
[0039] The liquid seeds of Ganoderma lucidum were inoculated in three different media respectively, the inoculum amount was 5% (V / V), placed on a shaker after inoculation, 25°C, 150 r / min, shaking culture for 10 days, and the mycelium was collected by filtration Determination of mycelium biomass and triterpene content, as well as the content of polysaccharides and polyphen...
Embodiment 3
[0052] A sugarcane juice functional drink, which is prepared by adding 100mL of the fermented puree I prepared in Example 2 and adding 10-30mL of the kombucha fermentation broth prepared in Example 1.
[0053] The appearance of the sugarcane juice functional beverage is yellow and opaque, and has special flavors of sugarcane juice, ganoderma lucidum and kombucha, and the content of ganoderma lucidum polysaccharide per 100mL is more than 200mg. This recipe is suitable for most people.
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