Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting unsaturated fatty acid from Asia grateloupia filicina

A technology of unsaturated fatty acid and Asiatic centipede, which is applied in the direction of fatty acid production, fatty acid chemical modification, and fat production, which can solve problems such as low efficiency and achieve the effect of improving extraction efficiency.

Active Publication Date: 2015-07-01
THE FIRST INST OF OCEANOGRAPHY SOA
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for extracting unsaturated fatty acids from Asian centipede algae, thereby solving the problem of low efficiency of the traditional extraction method of seaweed unsaturated fatty acids

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting unsaturated fatty acid from Asia grateloupia filicina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A. Rinse the Asian centipede algae, add cellulase (Beijing Shengshi Jiaming Technology Development Co., Ltd., 30000u / g, which contains C1 enzyme, Cx enzyme and β-glucosidase,) according to the ratio of mass ratio 1:100 50 ℃ for 2 hours, drained, dried, and then crushed to obtain centipede algae powder;

[0018] B. Add centipede algae residue powder to petroleum ether and soak for 24 hours, the mass volume ratio of centipede algae residue powder to petroleum ether is 1g:12ml, reflux in a water bath at 45-65°C for 2-4 hours, filter and centrifuge to obtain supernatant;

[0019] C, add 0.1M potassium hydroxide, ethanol saponification solution to the supernatant;

[0020] D. Add 1mol / L hydrochloric acid solution after saponification, adjust the pH value to 3.5-4, and the filtrate after suction filtration is unsaturated fatty acid.

[0021] 2. The detection method of unsaturated fatty acids is as follows:

[0022] A. Dissolve the extracted total unsaturated fat with 2-5ml ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting unsaturated fatty acid from an Asia grateloupia filicina. The method comprises the following steps: washing Asia grateloupia filicina clean; adding a composite cellulase according to the mass ratio of 1:100 and acting at the temperature of 50 DEG C for 2h; draining, drying and smashing to obtain the grateloupia filicina powder; adding the grateloupia filicina powder into petroleum ether, soaking for 24h, performing water bath backflow, filtering and centrifuging to obtain supernate; adding a saponified solution to the supernate, adding a hydrochloric acid solution by the end of saponification, and adjusting the PH value to 3.4-4 to obtain the unsaturated fatty acid. The gas chromatography shows that in the unsaturated fatty acid extracted from the grateloupia filicina pretreated by the composite cellulase, the content of timnodonic acid (C20:5) and eicosatetraenoic acid (C20:4) is greater than 75% of the total unsaturated fatty acid and is about 50% higher than that of the direct extraction process. A method for efficiently extracting the unsaturated fatty acid from the Asia grateloupia filicina is established for the first time and a scientific basis for the efficient comprehensive utilization of Asia grateloupia filicina is provided.

Description

technical field [0001] The invention belongs to the technical field of fatty acid extraction, and in particular relates to a method for efficiently extracting unsaturated fatty acids from centipede algae. Background technique [0002] The research results show that unsaturated fatty acids have important physiological activities such as anti-atherosclerosis, anti-thrombosis, anti-hyperlipidemia, anti-hypertension, and elimination of inflammation, so they are highly valued by people. However, unsaturated fatty acids cannot be synthesized in the human body and can only be supplemented through diet. Marine fish and fish oils are the main commercial sources of unsaturated fatty acids, but their suitability and safety have been questioned, so there is a need to find suitable potential energy sources to replace them. Seaweed has been reported to be lower in fat but higher in unsaturated fatty acids (PUFAs) than terrestrial organisms. Among them, red algae do not contain 22-carbon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C11B1/04C11B1/10C11B3/00C11C3/00
CPCC11B1/04C11B1/10C11B3/00C11B3/001C11B3/008C11C3/00
Inventor 李江
Owner THE FIRST INST OF OCEANOGRAPHY SOA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com