Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing high-concentration guar gum solution

Abstract

The invention discloses a method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing a high-concentration guar gum solution. The method is characterized by firstly preparing a guar gum solution with a citric acid-sodium citrate buffer solution, then controlling the temperature and time to carry out heating swelling, after cooling, adding beta-mannanase, xylanase and endoglucanase at the same time, fully mixing the materials uniformly and controlling the enzymolysis temperature and time to carry out degradation. The method has the effects of achieving rapid enzymolysis of guar gum at high concentration, obtaining the galactomannan oligosaccharide enzymatic hydrolysate and saving the drying and purifying costs during subsequent production of galactomannan oligosaccharide.

Description

technical field

[0001] The invention relates to a method for quickly preparing a high-concentration guar gum solution for enzymatic hydrolysis of oligogalactomannose, and belongs to the field of biotechnology. Background technique

[0002] Guar gum is a natural thickener and quality improver processed from the endosperm of guar beans, an annual herbaceous leguminous crop. The main component is galactomannan. Guar gum is a white or yellowish free-flowing powder, soluble in cold or hot water, and forms a jelly-like substance when it meets water, which can thicken rapidly. The backbone of the galactomannan structure is mannan linked together by β-(1-4) glycosidic bonds, and the branched chains are galactans linked by α-(1-6) glycosidic bonds. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molecules through glycosidic bonds. The molar ratio of galactose and mannose About ...