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Straw decomposition microbial inoculant and application thereof

A technology of bacteria agent and bacteria agent, which is applied in straw decomposing bacteria agent and its application field, can solve the problems of improper compounding ratio, weak pertinence, single microbial metabolism type and function, etc., and achieve rapid decomposition, lignin The effect of increasing decomposition rate and total decomposition rate

Active Publication Date: 2015-07-15
LESHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the decomposing bacteria agents for crop straw returning to the field have problems such as single microbial metabolism type and function, poor pertinence, and improper compounding ratio, which cannot achieve fast and efficient straw decomposition effects, and are difficult to promote and apply on a large scale

Method used

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  • Straw decomposition microbial inoculant and application thereof
  • Straw decomposition microbial inoculant and application thereof
  • Straw decomposition microbial inoculant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, the preparation of bacterial agent

[0044] 1. Activation

[0045] 1.1. Inoculate Aspergillus niger ZM-8 on No. 1 medium, place it in a 30° C. incubator and cultivate it for 3 days to obtain activated Aspergillus niger ZM-8 (Aspergillus niger ZM-8) CCTCC NO: M209125.

[0046] 1.2, Trichoderma pseudokoningii (Trichoderma pseudokoningii) CGMCC NO.3.6608 and Penicillium visidicatum (Penicillium visidicatum) CGMCC NO.3.5933 were inoculated on No. 2 medium respectively, placed in a 30°C incubator and cultivated for 3 days to obtain Activated Trichoderma pseudokoningii CGMCC NO. 3.6608 and Penicillium visidicatum CGMCC NO. 3.5933.

[0047] 1.3. Inoculate Clostridium xylanolyticum DSM No.6555, Pseudomonas sp. ACCC No.11691 and Bacillus subtilis ACCC No.03189 into 100ml No. 4 culture medium respectively In a 250ml Erlenmeyer flask with base, placed in a 30°C incubator for static culture for 3 days, the activated Clostridium xylanolyticum (Clostridium xylanolytic...

Embodiment 2

[0057] Embodiment 2, the decomposing effect of different bacterial agents on crop stalks in the culture medium

[0058] The following experiments were repeated 3 times for each treatment, and the results were averaged.

[0059] 1. Decomposition effect of different bacterial agents on wheat straw in culture medium

[0060] Wheat straw powder is obtained by crushing wheat straw to a particle size of 2-5 cm.

[0061] Configure wheat straw medium: solute and its concentration are: peptone 5g / L, yeast powder 1g / L, wheat straw powder 5g / L, NaCl 5g / L, K 2 HPO 4 1g / L, MgSO 4 ·7H 2 O 0.35g / L, CaCO 3 3g / L; solvent is water; pH7.2±0.1. Autoclaved at 121℃ for 15min.

[0062] 1.1. Control treatment area

[0063] Add 150 ml of the above-mentioned wheat straw medium into a 250 ml Erlenmeyer flask without adding any bacterial agent, and place it in a 30° C. incubator for static culture for 15 days. The cellulose decomposition rate, hemicellulose decomposition rate and lignin decompo...

Embodiment 3

[0083] Embodiment 3, the decomposing effect of different bacterial agents on crop stalks in soil

[0084] In this embodiment, the design methods of the determination experiments of the decomposition and cellulose decomposition rate, hemicellulose decomposition rate and lignin decomposition rate of four kinds of crop straws are the same, as follows: the experiment adopts random block design, and 3 repetitions are set. In each repeated area, 4 treatment areas were randomly set up, which were the control treatment area, the bacterial agent A treatment area, the bacterial agent B treatment area and the bacterial agent C treatment area. The area of ​​each treatment area is 0.5 mu.

[0085] The experiment was repeated three times, and the average value was taken.

[0086] In this embodiment, the soil humidity in the field experiment is 16%-18%, and the air temperature is 20-30°C.

[0087] 1. Decomposition effect of different bacterial agents on wheat straw in soil

[0088] The fi...

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Abstract

The invention discloses a straw decomposition microbial inoculant and application thereof. The straw decomposition microbial inoculant disclosed by the invention is composed of the following active components: Aspergillus niger, Trichoderma pseudokoningii, Penicillium viridicatum, xylan-solubilizing clostridium, pseudomonad and Bacillus subtilis. The straw rotten microbial inoculant has quick decomposition effect on crop straws. After the straw rotten microbial inoculant is applied for 7 days, the total decomposition rates of the wheat straws and rice straws are respectively up to 25.6% and 28.9%; and after 10 days, the total decomposition rates of the corn straws and rape straws are respectively up to 30.8% and 23.5%. After the straw rotten microbial inoculant is applied for 15 days, the total decomposition rates of the wheat straws and rice straws are respectively up to 60.1%, and the total decomposition rates of the corn straws and rape straws are respectively up to 63.8% and 57.7%.

Description

technical field [0001] The invention relates to a straw decomposing bacterial agent and its application in the biological field. Background technique [0002] Crop straw returning to the field is an important agricultural farming measure, which has positive significance for improving land use efficiency, maintaining soil organic matter content, and realizing ecological cycle agriculture. However, the main components of crop straw are cellulose (38-50%), hemicellulose (17%-32%) and lignin (15%-30%). These three components are closely combined with each other and have a compact structure. state is more difficult to decompose. Therefore, in order to achieve the ideal straw decomposition effect, it is necessary to strengthen the destruction of the structure in the straw in order to achieve the rapid decomposition of the straw. At present, most of the decomposing bacterial agents for crop straw returning to the field have problems such as single microbial metabolism type and fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F11/08C12R1/145C12R1/125C12R1/38C12R1/80C12R1/885C12R1/685
CPCC05F11/08C12N1/14C12N1/20
Inventor 马旭光江滔唐琼易志刚
Owner LESHAN NORMAL UNIV
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