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HPLC method for simultaneously determining organic acids, nucleosides and ephedrine in pinellia tuber

A technology of organic acid and ephedrine, which is applied in the field of medicine to achieve the effect of fast separation, good linear relationship and high efficiency of the method

Active Publication Date: 2015-07-15
CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no literature report on the simultaneous detection and quantification of these six organic acids, three nucleosides and ephedrine

Method used

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  • HPLC method for simultaneously determining organic acids, nucleosides and ephedrine in pinellia tuber
  • HPLC method for simultaneously determining organic acids, nucleosides and ephedrine in pinellia tuber

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Embodiment

[0021] Preparation of standard solution: Accurately weigh 50mg, 60mg, 63mg, 60mg, 10mg, 12mg, 16mg, 20mg of standard oxalic acid, citric acid, malic acid, succinic acid, uridine, guanosine, adenosine, and ephedrine, respectively, add water After dissolving and diluting, the concentrations of oxalic acid, citric acid, malic acid, succinic acid, uridine, guanosine, adenosine and ephedrine were respectively 74 μg·mL -1 , 178 μg·mL -1 , 189 μg·mL -1 , 178 μg·mL -1 , 12μg·mL -1 , 11 μg·mL -1 , 14 μg·mL -1 , 3μg·mL -1 . Accurately draw 0.1ml of formic acid and acetic acid standard solutions respectively, and after diluting with water, the concentration of formic acid and acetic acid is 226μg·mL -1 , 373μg·mL -1 Reference substance solution. Precisely draw 5ml of each control stock solution into a 50mL measuring bottle, shake well to obtain a mixed reference solution.

[0022] Preparation of standard curve: Precisely draw 0.1mL, 0.5mL, 1mL, 2mL, 4mL of the mixed standard so...

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Abstract

The invention belongs to the technical filed of traditional Chinese medicine composition analysis determination, and relates to an HPLC method for simultaneously determining the content of six organic acids, three nucleosides and ephedrine in pinellia tuber in a pinellia tuber medicinal material and preparation. The method is characterized in that a chromatographic column is a Boston Green ODS (4.6mm*150mm, 5[mu]m) chromatographic column, a phosphoric acid buffer-methanol solution is adopted as a mobile phase, gradient elution is carried out, the flow velocity is 1mL / min, the sample introduction amount is 20[mu]L, the column temperature is 30DEG C, and the detection wavelength is 210nm. A linear relationship among oxalic acid, formic acid, malic acid, acetic acid, citric acid, succinic acid, uridine, adenosine, guanosine and ephedrine in a suitable concentration range is good. The method is accurate, rapid and reliable, and can be used to effectively and simultaneously determine the content of oxalic acid, formic acid, malic acid, acetic acid, citric acid, succinic acid, uridine, adenosine, guanosine and ephedrine in a pinellia tuber extract product.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to an analysis technique for simultaneously determining six kinds of organic acids, three kinds of nucleosides and ephedrine in pinellia medicinal materials and preparations. The six organic acids are oxalic acid, formic acid, malic acid, acetic acid, citric acid and succinic acid; the three nucleosides are uridine, adenosine and guanosine. Background technique [0002] Pinellia is the dry tuber of Pinellia temate (thumb.) Breit., a plant of the family Araceae. It has the effects of drying dampness and reducing phlegm, reducing adverse reactions and relieving vomiting, and dispelling swelling and stagnation. It is widely used in clinical practice. [0003] Pinellia contains a variety of chemical components, mainly including organic acids, nucleosides, alkaloids and so on. Among them, the total free organic acids have the effects of relieving cough, eliminating phlegm, inhibiting tumor cell pr...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 赵华张严方张景勍雷婷婷
Owner CHONGQING MEDICAL UNIVERSITY
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