Tissue culture and rapid propagation method for euphorbia lathyris

A technology of Caplet group and caperia, which is applied in the field of tissue culture and rapid propagation of caperia, which can solve the problems of reducing the output of medicinal materials, and achieve the effect of saving the amount of seeds used and increasing the yield

Active Publication Date: 2015-07-22
黎媛
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] At present, Capper is mainly propagated by seeds, but its medicinal part is also seeds, which reduces the yield of its medicinal materials to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Obtaining sterile seedlings: select plump caperia seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, sterilize with 75% alcohol for 30 seconds, then sterilize with 0.1% mercury liter solution for 8 minutes, and finally use sterile After rinsing with water for 3 times, blot the surface moisture with sterile filter paper and inoculate it into the seed germination medium. After inoculation, it is placed in the light for 24 hours a day, the light intensity is 1000lx, the culture temperature is 25°C, and the relative air humidity is 75%. After 20 days of cultivation, the germination rate reaches 100%. The germination medium is: 1 / 2MS+0.02mg / L NAA+15g / L sucrose+3.5g / L agar, with a pH of 5.5.

[0021] (2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.5 cm with a scalpel, and inoculate them in the medium for callus ...

Embodiment 2

[0027] (1) Obtaining sterile seedlings: select plump caperia seeds, peel off the seed coat with surgical scissors in an ultra-clean workbench, disinfect with 75% alcohol for 45 seconds, then disinfect with 0.1% mercury liter solution for 10 minutes, and finally use sterile Rinse with water 5 times, blot the surface moisture with sterile filter paper and inoculate into seed germination medium. After inoculation, it is placed in the light for 24 hours a day, the light intensity is 1500lx, the culture temperature is 25°C, and the relative air humidity is 75%. After 20 days of cultivation, the germination rate reaches 100%. The germination medium is: 1 / 2MS+0.05mg / L NAA+18g / L sucrose+3.8g / L agar, with a pH of 5.7.

[0028](2) Callus induction: When the cotyledons of the sterile seedlings have just unfolded, take the shoot tips of the sterile seedlings as explants, cut them into a length of about 0.5 cm with a scalpel, and inoculate them in the medium for callus induction nourish. ...

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Abstract

The invention discloses a tissue culture and rapid propagation method for euphorbia lathyris. The euphorbia lathyris is a biennial herbaceous plant of euphorbia of euphorbiaceae, and the seeds are also named semen euphorbiae lathyridis. The euphorbia lathyris is a traditional Chinese medicine of China, has the functions of relieving water retention and eliminating swelling, relieving blood and removing stasis, is a special-effect medicine for treating advanced schistosomiasis, and has an anti-tumor effect. The euphorbia lathyris is also an ideal energy plant. The oil content of the seed reaches up to about 45%, and the euphorbia lathyris is one of the excellent raw materials for preparing biodiesel. By taking a euphorbiae lathyridis seed as an explant, an euphorbiae lathyridis in vitro re-plant is successfully obtained through processes including callus induction, proliferation, differentiation, rooting, acclimatization and transplanting and the like and a fast propagation technical system for tissue culture of euphorbia lathyris is established, so that the use level of the seeds in cultivation production is saved, the output of medicinal materials is improved, and a foundation is laid for further conducting a study on genetic transformation of the euphorbia lathyris.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Caperia spp. Background technique [0002] Capper ( Euphorbia lathyris L.), also known as Qianjinzi, Xiaocrodou, etc., is a biennial herb of the genus Euphorbiaceae, widely distributed in more than 10 provinces and cities including Zhejiang, Guangxi, Tibet, and Jilin in my country. It is a traditional Chinese medicine in my country. It has the effects of dispelling water, reducing swelling, breaking blood and dispelling stagnation. It is a special drug for treating advanced schistosomiasis and has anti-tumor effects. Capper is also an ideal energy plant, and its seed oil content is as high as about 45%, which is one of the high-quality raw materials for preparing biodiesel. In recent years, Caper caper as an energy plant has been widely valued in the world, and through the collection of ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冯文杰
Owner 黎媛
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