33 results about "Euphorbia acanthothamnos" patented technology

The invention relates to the technical field of grassland ecological remote sensing intelligent detection, in particular to a toxic plant euphorbia fischeriana identification and spatial positioning method based on YOLOv8. The method comprises the following steps: optimizing a trunk feature extraction network, a neck network and a convolution module of a YOLOv8 network model to form an improved RFC-YOLOv8 network model; compared with the prior art, the method not only improves the detection precision of YOLOv8, but also enables the network model to be lighter, and improves the reasoning speed, thereby achieving the quick and accurate recognition and positioning of euphorbia fischeriana in a natural grassland, and finally achieving the spatial distribution drawing of euphorbia fischeriana.

The present invention and the technical field of natural medicines particularly relate to an enantiopysenane-type diterpene lactone compound and a preparation method and application thereof. The enantiopysenane-type diterpene lactone compound has a structure as shown in Formula I, and the preparation method comprises the following steps: using the dried root of Euphorbia chamaejasme as a raw material, extracting by filtration and concentrating to obtain an extract and suspending it in water to obtain a suspension, extracting and concentrating to obtain an extract; taking the extract and eluting and purifying it by ODS liquid chromatography after gradient elution to obtain the compound shown in Formula I. The present invention realizes the extraction of a 6 / 6 / 6 / 7 ring system enantiopysenane-type diterpene lactone compound with acetylcholinesterase inhibition from Euphorbia chamaejasme for the first time, and has great development value as a new drug for the prevention or treatment of Alzheimer's disease. The design idea of ​​such a compound also provides a new idea and approach for the development of a new drug for the prevention or treatment of Alzheimer's disease.

The invention belongs to the technical field of biochemistry, and particularly provides a carbonic anhydrase binding ligand which comprises at least one of euphorbia alcohol type diterpene and olivone type diterpene. The carbonic anhydrase binding ligand has the effects of resisting tumors, viruses, fungi and inflammation, treating edema, glaucoma, senile dementia, hyperammonemia and the like. An enzymology experiment shows that the compound is a carbonic anhydrase agonist and also has industrial applications of capturing and fixing carbon dioxide, accelerating mineralization of biological tissues and corals and the like. The invention also provides a preparation method of the carbonic anhydrase binding ligand, which takes euphorbia kansui as a raw material, is simple to prepare and provides a new way for application of euphorbia kansui.

The present invention belongs to the field of medicine, and specifically relates to a Euphorbia licorice supramolecular hydrogel and a preparation method and use thereof. The supramolecular hydrogel comprises Euphorbia ethyl acetate extract and glycyrrhizic acid, and the Euphorbia ethyl acetate extract and the glycyrrhizic acid interact with each other to form nanoparticles by self-assembly. The preparation method of the Euphorbia licorice supramolecular hydrogel comprises the following steps: weighing the Euphorbia ethyl acetate extract and glycyrrhizic acid and placing them in a round-bottom flask, adding an appropriate amount of deionized water, and decocting for 0.5 to 2 hours. Experimental results show that the Euphorbia licorice supramolecular hydrogel of the present invention has a good anti-hepatoma ascites effect and has excellent safety compared with conventional chemotherapy drugs.

The present invention relates to the field of extraction of natural compounds, and specifically to an ursane-type triterpenoid compound, its preparation method, and application. The ursane-type triterpenoid compound of the present invention can be extracted from the Euphorbia genus medicinal plant Euphorbia ulmoides. The ursane-type triterpenoid compound can significantly inhibit the survival of liver cancer cells. The structural formula of the ursane-type triterpenoid compound of the present invention is:

The invention discloses external ointment for treating psoriasis, which is prepared from the following raw materials in parts by weight: 17 to 21 parts of gallnut, 14 to 18 parts of datura stramonium flower, 19 to 23 parts of euphorbia scabra, 18 to 22 parts of eucalyptus leaf, 18 to 22 parts of millettia divaricata, 19 to 23 parts of paris polyphylla, 1 to 5 parts of verdigris and 1 to 5 parts of minium. The preparation method comprises the following steps: respectively putting gallnut, datura flower, euphorbia scabra, eucalyptus leaf, millettia divaricata, paris polyphylla, verdigris and minium into a grinding machine, and grinding into fine powder; the preparation method comprises the following steps: weighing fine powder of gallnut, datura stramonium flower, euphorbia scabra, eucalyptus leaf, millettia divaricata, paris polyphylla, verdigris and minium, placing in a pot, mixing, uniformly stirring, pouring water and vinegar according to a ratio of 1: 1, decocting and concentrating, adding honey, and stirring while cooling to normal temperature, thereby obtaining the traditional Chinese medicine for treating psoriasis, which has the advantages of easily available raw materials, simple preparation process, no side effect, good treatment effect on psoriasis, and no toxic or side effect. Good popularization prospects and medical economic values are realized.

The invention belongs to the technical field of medicinal chemistry, relates to a skeleton rearrangement product of an euphorbia factor L1 and application of the skeleton rearrangement product in pharmacy, and particularly relates to a skeleton structure rearrangement derivative of the euphorbia factor L1 and application of the skeleton structure rearrangement derivative in preparation of anti-tumor multidrug resistance drugs. According to the present invention, the skeleton isomerization reaction is performed on EFL1 by using p-TsOH to generate the derivative compound 1 having the 5 / 7 / 7 / 4 tetracyclic combined skeleton rearrangement, and the test results show that the derivative compound 1 has significantly good anti-tumor multidrug resistance activity compared to the raw material EFL1, and can be further used for preparing the anti-tumor multidrug resistance drug. The skeleton compound disclosed by the invention has important drug development value for resisting tumor multidrug resistance activity. # imgabs0 #

This invention belongs to the field of biomedicine, specifically relating to the application of APOL2 (Apolipoprotein L2) inhibitors in the preparation of products for treating liver fibrosis. The inventors isolated a series of natural tetrodopane-type diterpenes from *Euphorbia pekinensis*, a plant in the Euphorbiaceae family. Anti-liver fibrosis-related activity tests on this series of diterpenes revealed that they significantly inhibited the expression of fibronectin, type I collagen, and α-smooth muscle actin in LX-2 cells. In animal studies, their therapeutic effect was superior to that of pirfenidone, a phase II clinical trial drug for treating liver fibrosis, and they showed no significant toxicity. Mechanistic studies showed that TD1 is an APOL2 inhibitor, and knocking out APOL2 protein in vivo can alleviate the progression of liver fibrosis. In summary, this series of tetrodopane-type diterpenes, especially TD1, shows promise as a candidate drug for treating liver fibrosis and provides a new target for researching novel drugs for treating liver fibrosis.

The present application relates to a kaurane diterpenoid compound D-33 extracted and separated from the roots of Euphorbia griffithii Hook., a preparation method and uses thereof. Bioactivity tests show that the compound D-33 has a significant weight loss effect in a high-fat diet-induced obese mouse model, can effectively activate uncoupling protein 1 (UCP1) in vitro, and can be used for preparing a thermogenic anti-obesity drug.

The present invention relates to a pharmaceutical composition and a health functional food for the prevention or treatment of thrombosis containing an extract of Euphorbia hypericifolia, and more specifically, to a pharmaceutical composition and a health functional food for the prevention or treatment / improvement of thrombosis through inhibition of blood coagulation containing an extract of Euphorbia hypericifolia as an active ingredient having potent thrombin inhibitory and blood coagulation factor inhibitory activity. The extract of *Euphorbia hypericifolia*, used as an active ingredient in the pharmaceutical composition and health functional food for the prevention or treatment of thrombosis according to the present invention, exhibits excellent antithrombotic activity through strong inhibition of thrombus-related enzymes such as thrombin and blood coagulation factors. At the same time, it does not exhibit any hemolytic activity against human red blood cells, has excellent thermal stability, and does not show loss of blood coagulation factor inhibitory effects or thrombus-related enzyme inhibitory effects even under acidic conditions of pH 2 and in plasma. Therefore, it is expected to be usable for the prevention and treatment of thrombosis, such as ischemic stroke and hemorrhagic stroke, through the improvement of blood circulation. Furthermore, since the active ingredient has the excellent effect of being processed into various forms such as extract, powder, pills, and tablets, allowing for formulation into a form suitable for regular consumption, this invention is highly useful for the pharmaceutical and food industries.

The invention relates to the field of extraction of natural compounds, in particular to ursane triterpenoids as well as a preparation method and application thereof. The ursane type triterpenoid compound can be extracted from euphorbia medicinal plant euphorbia ebracteolata, and the ursane type triterpenoid compound can obviously inhibit the survival of liver cancer cells. The structural formula of the ursane type triterpenoid compound disclosed by the invention is # imgabs0 #.

A new and distinct cultivar of Euphorbia plant named ‘BONPRI 16P5’, characterized by its relatively compact, upright and mounded plant habit; vigorous growth habit; freely branching habit; relatively early flowering habit; inflorescences with purplish pink-colored flower bracts with lighter purplish pink-colored marginal edges; inflorescences with tight cyathia clusters; and good post-production longevity.

The invention discloses a composite culture medium and application thereof in rapid propagation of euphorbia colorata. The starting culture medium takes an MS culture medium as a basic culture medium and contains 6-benzyl purine, naphthylacetic acid, cane sugar and agar; the subculture medium takes an MS (Murashige and Skoog) culture medium as a basic culture medium, and contains 6-benzyl purine, methylthiodazine, thidiazuron, cane sugar and agar; the rooting culture medium takes a 1 / 2 MS culture medium as a basic culture medium, and contains indolebutyric acid and uniconazole, or diethyl aminoethyl hexanoate and isopentene adenine. By adopting the composite culture medium disclosed by the invention, the euphorbia colorata can be rapidly propagated. Meththiodazine and thidiazuron in the components of the culture medium are used in a combined mode, the propagation coefficient in the subculture stage is increased, synergistic interaction is achieved, and indolebutyric acid and uniconazole or diethyl aminoethyl hexanoate and isopenteneadenine are used in a combined mode, so that the number of roots and the average root length in the rooting stage are increased.

A system for producing plant latex-based biopesticide formulations from Euphorbiaceae species for controlling sucking pests, comprising: (a) a latex collection unit configured to collect latex from Euphorbia antiquorum and Euphorbia tirucalli plants; (b) a lyophilisation unit configured to convert the collected latex into powder form by freeze-drying; c) a formulation preparation unit configured to produce emulsifiable concentrate formulations containing 2.5% active ingredient using environmentally friendly solvents and optimised surfactant systems; (d) a stability test unit configured to evaluate the stability parameters of the formulation, including emulsion stability, creaming, sedimentation and phase separation; and (e) a bioefficacy assessment unit configured to assess pest control efficacy against sucking pests, including aphids and thrips.

The invention relates to the technical field of anti-tumor compounds, and provides a phenylpropanoid compound as well as an extraction and purification method and application thereof. A phenylpropanoid compound (euphorbia ebracteolata) is extracted, separated and identified from a traditional Chinese medicine stellera chamaejasme (euphorbia ebracteolata) for the first time, and in-vitro anti-tumor effect research finds that the compound (euphorbia ebracteolata) can inhibit proliferation of HL-60, SMMC-7721 and SW480 cell lines, and especially has higher activity of inhibiting SMMC-7721 liver cancer cells; meanwhile, the compound euphberarin A can inhibit the SMMC-7721 hepatoma carcinoma cell cycle from being retarded in a G2 / M period, and is concentration-dependent. In conclusion, the novel phenylpropanoid compound provided by the invention is high in anti-tumor activity, and has a wide application prospect in the field of anti-tumor drugs, especially in the field of anti-liver cancer drugs.

The invention relates to Euphorbia honey, pollen and propolis, which can be used individually or in mixtures for the prevention and / or treatment of infertility.

The invention relates to the technical field of plant biology, and particularly discloses an agrobacterium rhizogenes-mediated tissue-culture-free euphorbia kansui transformation method, which is technically characterized by comprising the following steps: S1, constructing a pCAMBIA1300:: 35S-EkHMGR-GFP recombinant vector by using a specific primer; s2, establishing a euphorbia kansui regeneration system as an identification basis for obtaining a transgenic plant from an expanded root segment; s3, infecting wounds through agrobacterium rhizogenes K599 to induce hairy roots, and regenerating transgenic plants by utilizing expanded root segments. A set of rapid and stable euphorbia kansui genetic transformation system which is mediated by agrobacterium rhizogenes and does not need tissue culture is established, recombinant plasmids carrying a target gene EkHMGR are introduced into euphorbia kansui by the agrobacterium rhizogenes to obtain transgenic hairy roots, and positive plants obtained by culturing expanded root segments are transplanted. The transformation method constructed by the invention has potential application value on genetic transformation of euphorbia kansui and also has important reference value on genetic transformation of other plants, and research results provide theoretical guidance for promoting synthesis and accumulation of euphorbia kansui key medicinal component euphorbia dienol.

The present invention and the technical field of natural medicines particularly relate to an enantiopysenane-type norditerpene lactone compound and a preparation method and application thereof. The enantiopysenane-type norditerpene lactone compound has a structure as shown in Formula I, and the preparation method comprises the following steps: using the dried root of Euphorbia chamaejasme as a raw material, extracting by filtration and concentrating to obtain an extract and suspending it in water to obtain a suspension, extracting and concentrating to obtain an extract; taking the extract and eluting and purifying it by ODS column chromatography after gradient elution to obtain the compound shown in Formula I. The present invention realizes the extraction of an enantiopysenane-type norditerpene lactone compound with acetylcholinesterase inhibition from Euphorbia chamaejasme for the first time, and has great development value as a new type of drug for the prevention or treatment of Alzheimer's disease. The design idea of ​​such a compound also provides a new idea and approach for the development of a new type of drug for the prevention or treatment of Alzheimer's disease.

The invention discloses a Tibetan medicine anti-inflammation inunction preparation and a preparation method thereof, and belongs to the technical field of Tibetan medicines, and the Tibetan medicine anti-inflammation inunction preparation comprises rhubarb, alkali, oxytropis verticillata, herba achyranthis bidentatae, stellera chamaejasme, euphorbia macrocarpa, goethite, folium ilicis scandens, rock brain, volundaisy, gypsum rubrum, rhubarb, thalictrum aquilegifolium, codonopsis alpina, Perot, semen cassiae, abelmoschus esculentus seeds and lagotis diffusa. The medicament has the effects of promoting blood circulation to remove blood stasis, dispelling wind, eliminating dampness, relaxing tendons, dredging collaterals, diminishing inflammation and relieving pain. The traditional Chinese medicine composition can be used for treating joint swelling and pain, limb stiffness, limited activity, hand and foot spasm, neuralgia and other symptoms caused by vasculitis, varicosity, peripheral neuritis, rheumatism, rheumatoid arthritis, gout, fracture and the like, and has effective swelling and pain relieving effects.

A new and distinct cultivar of Euphorbia plant named ‘Urge to Splurge’ characterized by compact stems with obovate, bluish-green, glaucous foliage that develops a reddish tint in the summer and fall. The flowers are surrounded by bright yellow bracts and cover the plant in spring.

The invention relates to the technical field of medicine, in particular to application of euphorbia fischeriana extract in preparation of medicine for preventing and treating ulcerative colitis. The preparation method of the euphorbia fischeriana extract comprises the following steps: cleaning collected euphorbia fischeriana, removing surface soil and other impurities, then putting the euphorbia fischeriana in an electrothermal blowing drying box at 40 DEG C for drying, crushing the euphorbia fischeriana by using a traditional Chinese medicine crusher for 15 minutes after drying, and removing uncrushed parts by using a 50-mesh screen to obtain the euphorbia fischeriana extract. Placing the treated sample in a sample bag and storing the sample in a refrigerator at-20 DEG C; mixing 100g of dried euphorbia fischeriana powder with 1L of 95% ethanol, extracting for 1h by using a condensation reflux method under the water bath condition of 40 DEG C, and filtering an alcohol extracting solution by using filter paper and a negative pressure filtering device to remove residual substances in the alcohol extracting solution;

The invention discloses euphorbia fischeriana terpene A as well as a preparation method and application thereof, and relates to a novel substance capable of resisting breast cancer in vitro as well as a method and application thereof. The invention provides a novel substance with in-vitro anti-breast cancer activity and a preparation method and application thereof. The molecular formula of the euphorbia fischeriana terpene A is C21H34O13. The preparation method comprises the following steps: 1, carrying out cold-soaking extraction on euphorbia fischeriana by using ethanol; 2, dispersing the extract from which the ethanol is recovered with water, and extracting; and 3, carrying out repeated silica gel column chromatography separation on the ethyl acetate extraction layer, and then utilizing high performance liquid chromatography to obtain a monomeric compound which is identified as a new compound through spectroscopy and named Euphorbia fischeriana terpene A. The invention also discloses a preparation method of the Euphorbia fischeriana terpene A. The euphorbia fischeriana terpene A is applied to preparation of anti-breast cancer drugs. The IC50 of the euphorbia fischeriana terpene A to breast cancer MCF-7 cells, the IC50 of the euphorbia fischeriana terpene A to ZR-75-1 cells and the IC50 of the euphorbia fischeriana terpene A to MDA-MB-231 cells are 25.4 [mu] mol / L, 19.2 [mu] mol / L and 21.9 [mu] mol / L respectively, and it is indicated that the euphorbia fischeriana terpene A has The invention belongs to the field of research on effective components of natural medicines.

The euphorbia hupehensis phenolic acid compound (EHPA) (the compound as shown in the formula I) is separated from the root of euphorbia hupehensis for the first time, the separation and purification method is simple, the compound is high in safety and non-toxic to cells and animals through determination, and the compound is found to have antiviral activity through activity test, so that the compound can be used for preparing the anti-virus drug. The compound has an inhibiting effect on viruses such as porcine epidemic diarrhea virus (PEDV) and porcine D-coronavirus (PDCoV) which cause porcine diarrhea, can be used for preparing antiviral drugs, and has relatively high medicinal value and application prospect.

The present application relates to a kind of macrocyclic diterpenoids isolated from Euphorbia tirucalli and method and application, after the above-ground part of Euphorbia tirucalli is crushed, at room temperature, with ethanol extraction, solvent is evaporated under reduced pressure to obtain Euphorbia tirucalli crude extract extract, again, the crude extract extract is dispersed with water, and ethyl acetate is added to extract, until the organic phase is colorless, the organic phase layer is combined, and solvent is evaporated under reduced pressure to obtain ethyl acetate extract extract;Again, by normal phase silica gel column, reverse phase C 18 8 pseudo-alangui macrocyclic diterpenoids are obtained by column chromatography, and the anti-inflammatory, antitumor and multidrug resistance reversal activity of the 8 compounds are determined, and the results show that the 8 pseudo-alangui macrocyclic diterpenoids obtained have different degrees of anti-inflammatory, antitumor and multidrug resistance activity, and have potential for preparing related drugs.

The invention relates to crotonane diterpenoid glycoside as well as a derivative, a preparation method and application thereof. The invention relates to the technical field of medicines, and particularly discloses crotonane diterpenoid glycoside separated from a traditional medicine radix euphorbiae lantu (euphorbia fischeriana), a derivative of crotonane diterpenoid glycoside, a preparation method and application of crotonane diterpenoid glycoside to anti-inflammatory drugs. The structures of the compounds are identified through infrared spectroscopy, high-resolution mass spectrometry, nuclear magnetic resonance and other technologies, beta-D-glucoside of crotonane diterpenes is used as a basic skeleton, the compounds comprise three new compounds, and the compounds 2-4 have potential anti-inflammatory activity. In addition, the preparation method disclosed by the invention is simple and easy to implement and good in reproducibility, and crotonane diterpenoid glycoside and derivatives thereof with relatively high purity can be rapidly obtained.

A new and distinct cultivar of Euphorbia plant named ‘Doeupstaduwhifla25’, characterized by its relatively compact, mounding to outwardly spreading and eventually semi-trailing plant habit; freely branching habit; freely flowering habit; and inflorescences with multiple white-colored flower bracts.

The invention relates to a method for separating sesquiterpenoids from euphorbia euphorbia and application of the sesquiterpenoids, which comprises the following steps: crushing the overground part of euphorbia euphorbia, extracting by using ethanol at room temperature, evaporating a solvent under reduced pressure to dryness to obtain an euphorbia euphorbia crude extract, dispersing the crude extract by using water, adding ethyl acetate for extraction until an organic phase is colorless, and filtering to obtain the euphorbia euphorbia sesquiterpenoids. Combining organic phase layers, and evaporating the solvent to dryness under reduced pressure to obtain ethyl acetate extract; the method comprises the following steps of: firstly, performing normal-phase silica gel column chromatography and reversed-phase C18 column chromatography to obtain six carrot alkane type sesquiterpenoids, and performing anti-inflammatory and anti-tumor activity determination on the six compounds, and the result shows that the six separated carrot alkane type sesquiterpenoids have different degrees of anti-inflammatory and anti-tumor activity and have the potential for preparing related medicines.

According to the EHPA injection preparation for treating porcine viral diarrhea, Euphorbia hupehensis phenolic acid compounds (EHPA) are separated from roots of Euphorbia hupehensis for the first time and prepared into the injection preparation, after optimization, the stability of the injection preparation is high, the quality and characters of products within the shelf life are stable, the EHPA injection preparation is safe to animals, and animal experiments show that the EHPA injection preparation has good clinical application prospects. The compound has a good control effect on diarrhea caused by PEDV and / or PDCoV infection, can effectively reduce death of infected animals, and has an excellent treatment effect.

The invention provides a diterpenoid compound extracted from euphorbia fischeriana as well as a preparation method and application of the diterpenoid compound, and belongs to the technical field of plant medicines. The diterpenoid compound (A3710) in the medicinal material euphorbia fischeriana is found for the first time, the diterpenoid compound (A3710) has a remarkable anti-lung cancer effect, and the cytotoxicity IC50 value of the diterpenoid compound (A3710) to lung cancer cells reaches the nanomole level; in a mouse subcutaneous transplantation tumor model of cell line-derived allograft tumor and patient-derived tumor xenotransplantation, the diterpenoid compound A3710 can effectively inhibit the growth of lung cancer cells, and shows a clear anti-lung cancer drug effect. The invention further provides a method for preparing the diterpenoid compound A3710 from the medicinal material euphorbia fischeriana, the extraction rate of the diterpenoid compound A3710 prepared according to the method can reach 0.05% or above, and operation is convenient and fast.

The invention belongs to the technical field of euphorbia filiformis seedling culture, and particularly relates to a rapid seedling culture method of euphorbia filiformis, which comprises the following steps: explant sterilization treatment, callus induction, callus differentiation, strong seedling culture, acclimatization and transplantation and transplantation management. According to the method, tender leaves of euphorbia filiformis are selected as explants, rapid seedling culture of euphorbia filiformis is carried out on the explants through the steps of callus induction, callus differentiation, strong seedling culture, seedling hardening and the like, and an induction culture medium, a differential culture medium, a strong seedling culture medium and the like in the seedling culture process are optimized and improved. The induction effect, growth coefficient and rooting rate of calluses can be improved, cultivated seedlings can be robust and grow well, the purposes of effectively controlling the production cost and rapidly producing seedlings are achieved, and a foundation can be laid for large-scale production of euphorbia filiformis.