11 results about "Euphorbia acanthothamnos" patented technology

This invention belongs to the field of biomedicine, specifically relating to the application of APOL2 (Apolipoprotein L2) inhibitors in the preparation of products for treating liver fibrosis. The inventors isolated a series of natural tetrodopane-type diterpenes from *Euphorbia pekinensis*, a plant in the Euphorbiaceae family. Anti-liver fibrosis-related activity tests on this series of diterpenes revealed that they significantly inhibited the expression of fibronectin, type I collagen, and α-smooth muscle actin in LX-2 cells. In animal studies, their therapeutic effect was superior to that of pirfenidone, a phase II clinical trial drug for treating liver fibrosis, and they showed no significant toxicity. Mechanistic studies showed that TD1 is an APOL2 inhibitor, and knocking out APOL2 protein in vivo can alleviate the progression of liver fibrosis. In summary, this series of tetrodopane-type diterpenes, especially TD1, shows promise as a candidate drug for treating liver fibrosis and provides a new target for researching novel drugs for treating liver fibrosis.

The present application relates to a kaurane diterpenoid compound D-33 extracted and separated from the roots of Euphorbia griffithii Hook., a preparation method and uses thereof. Bioactivity tests show that the compound D-33 has a significant weight loss effect in a high-fat diet-induced obese mouse model, can effectively activate uncoupling protein 1 (UCP1) in vitro, and can be used for preparing a thermogenic anti-obesity drug.

The invention relates to the technical field of plant biology, and particularly discloses an agrobacterium rhizogenes-mediated tissue-culture-free euphorbia kansui transformation method, which is technically characterized by comprising the following steps: S1, constructing a pCAMBIA1300:: 35S-EkHMGR-GFP recombinant vector by using a specific primer; s2, establishing a euphorbia kansui regeneration system as an identification basis for obtaining a transgenic plant from an expanded root segment; s3, infecting wounds through agrobacterium rhizogenes K599 to induce hairy roots, and regenerating transgenic plants by utilizing expanded root segments. A set of rapid and stable euphorbia kansui genetic transformation system which is mediated by agrobacterium rhizogenes and does not need tissue culture is established, recombinant plasmids carrying a target gene EkHMGR are introduced into euphorbia kansui by the agrobacterium rhizogenes to obtain transgenic hairy roots, and positive plants obtained by culturing expanded root segments are transplanted. The transformation method constructed by the invention has potential application value on genetic transformation of euphorbia kansui and also has important reference value on genetic transformation of other plants, and research results provide theoretical guidance for promoting synthesis and accumulation of euphorbia kansui key medicinal component euphorbia dienol.

The invention discloses a Tibetan medicine anti-inflammation inunction preparation and a preparation method thereof, and belongs to the technical field of Tibetan medicines, and the Tibetan medicine anti-inflammation inunction preparation comprises rhubarb, alkali, oxytropis verticillata, herba achyranthis bidentatae, stellera chamaejasme, euphorbia macrocarpa, goethite, folium ilicis scandens, rock brain, volundaisy, gypsum rubrum, rhubarb, thalictrum aquilegifolium, codonopsis alpina, Perot, semen cassiae, abelmoschus esculentus seeds and lagotis diffusa. The medicament has the effects of promoting blood circulation to remove blood stasis, dispelling wind, eliminating dampness, relaxing tendons, dredging collaterals, diminishing inflammation and relieving pain. The traditional Chinese medicine composition can be used for treating joint swelling and pain, limb stiffness, limited activity, hand and foot spasm, neuralgia and other symptoms caused by vasculitis, varicosity, peripheral neuritis, rheumatism, rheumatoid arthritis, gout, fracture and the like, and has effective swelling and pain relieving effects.

The euphorbia hupehensis phenolic acid compound (EHPA) (the compound as shown in the formula I) is separated from the root of euphorbia hupehensis for the first time, the separation and purification method is simple, the compound is high in safety and non-toxic to cells and animals through determination, and the compound is found to have antiviral activity through activity test, so that the compound can be used for preparing the anti-virus drug. The compound has an inhibiting effect on viruses such as porcine epidemic diarrhea virus (PEDV) and porcine D-coronavirus (PDCoV) which cause porcine diarrhea, can be used for preparing antiviral drugs, and has relatively high medicinal value and application prospect.

The present application relates to a kind of macrocyclic diterpenoids isolated from Euphorbia tirucalli and method and application, after the above-ground part of Euphorbia tirucalli is crushed, at room temperature, with ethanol extraction, solvent is evaporated under reduced pressure to obtain Euphorbia tirucalli crude extract extract, again, the crude extract extract is dispersed with water, and ethyl acetate is added to extract, until the organic phase is colorless, the organic phase layer is combined, and solvent is evaporated under reduced pressure to obtain ethyl acetate extract extract;Again, by normal phase silica gel column, reverse phase C 18 8 pseudo-alangui macrocyclic diterpenoids are obtained by column chromatography, and the anti-inflammatory, antitumor and multidrug resistance reversal activity of the 8 compounds are determined, and the results show that the 8 pseudo-alangui macrocyclic diterpenoids obtained have different degrees of anti-inflammatory, antitumor and multidrug resistance activity, and have potential for preparing related drugs.

The invention relates to crotonane diterpenoid glycoside as well as a derivative, a preparation method and application thereof. The invention relates to the technical field of medicines, and particularly discloses crotonane diterpenoid glycoside separated from a traditional medicine radix euphorbiae lantu (euphorbia fischeriana), a derivative of crotonane diterpenoid glycoside, a preparation method and application of crotonane diterpenoid glycoside to anti-inflammatory drugs. The structures of the compounds are identified through infrared spectroscopy, high-resolution mass spectrometry, nuclear magnetic resonance and other technologies, beta-D-glucoside of crotonane diterpenes is used as a basic skeleton, the compounds comprise three new compounds, and the compounds 2-4 have potential anti-inflammatory activity. In addition, the preparation method disclosed by the invention is simple and easy to implement and good in reproducibility, and crotonane diterpenoid glycoside and derivatives thereof with relatively high purity can be rapidly obtained.

A new and distinct cultivar of Euphorbia plant named ‘Doeupstaduwhifla25’, characterized by its relatively compact, mounding to outwardly spreading and eventually semi-trailing plant habit; freely branching habit; freely flowering habit; and inflorescences with multiple white-colored flower bracts.

According to the EHPA injection preparation for treating porcine viral diarrhea, Euphorbia hupehensis phenolic acid compounds (EHPA) are separated from roots of Euphorbia hupehensis for the first time and prepared into the injection preparation, after optimization, the stability of the injection preparation is high, the quality and characters of products within the shelf life are stable, the EHPA injection preparation is safe to animals, and animal experiments show that the EHPA injection preparation has good clinical application prospects. The compound has a good control effect on diarrhea caused by PEDV and / or PDCoV infection, can effectively reduce death of infected animals, and has an excellent treatment effect.

The invention provides a diterpenoid compound extracted from euphorbia fischeriana as well as a preparation method and application of the diterpenoid compound, and belongs to the technical field of plant medicines. The diterpenoid compound (A3710) in the medicinal material euphorbia fischeriana is found for the first time, the diterpenoid compound (A3710) has a remarkable anti-lung cancer effect, and the cytotoxicity IC50 value of the diterpenoid compound (A3710) to lung cancer cells reaches the nanomole level; in a mouse subcutaneous transplantation tumor model of cell line-derived allograft tumor and patient-derived tumor xenotransplantation, the diterpenoid compound A3710 can effectively inhibit the growth of lung cancer cells, and shows a clear anti-lung cancer drug effect. The invention further provides a method for preparing the diterpenoid compound A3710 from the medicinal material euphorbia fischeriana, the extraction rate of the diterpenoid compound A3710 prepared according to the method can reach 0.05% or above, and operation is convenient and fast.

The invention discloses crotonane-type diterpenoid compounds in euphorbia macrocarpa as well as an extraction method and application thereof, belongs to the field of traditional Chinese medicine extraction, and particularly relates to crotonane-type diterpenoid compounds which are separated from euphorbia macrocarpa and have structural formulas shown as (I), (II) and (III), isomers of the compounds and pharmaceutically acceptable salts of the compounds. The compound has a protective effect on BV-2 cell injury induced by H2O2, or a pharmaceutical composition containing the compound can be used for preparing drugs for resisting oxidative stress injury, and groups in the compound are described in the claims and the specification.