Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of decellularized submandibular gland matrix material and its preparation method

A matrix material and submandibular gland technology, applied in the field of decellularized submandibular gland matrix material and its preparation, can solve the problems of extracellular matrix biotoxicity, damage to the structural integrity and biological activity of cell matrix, unfavorable three-dimensional structure reconstruction, etc., and achieve good cell differentiation The effect of proliferation environment, simple method and good mechanical properties

Active Publication Date: 2017-05-17
SUN YAT SEN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the strong elution effect of SDS, the structural integrity and biological activity of the cell matrix are largely destroyed. In addition, the extracellular matrix prepared by using SDS has potential biotoxicity, which is not conducive to the reconstruction of the three-dimensional structure of the tissue by the matrix material.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of decellularized submandibular gland matrix material and its preparation method
  • A kind of decellularized submandibular gland matrix material and its preparation method
  • A kind of decellularized submandibular gland matrix material and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Production of mouse submandibular gland ECM

[0041] (1) Material collection: the submandibular gland tissues of healthy mice were selected, cut into small pieces of 1 cm square, and washed twice with PBS. As for the EP tube, store it in a -80°C refrigerator for later use.

[0042] (2) Broken cells: Take out the tissue block from the -80°C refrigerator, thaw it in a 37°C water bath, and then put it back into the -80°C refrigerator for 30 minutes, and repeat the freezing and thawing process 3 times.

[0043] (3) Disinfection and preparation: Place the tissue block in 75% alcohol, suspend the submandibular gland tissue block with sutures, hang it in a 250ml jar, and place a 1cm magnetic stirring bar at the bottom of the bottle.

[0044] (4) Pre-cleaning and crushing: add 250ml of sterile third-grade water, and treat on a magnetic stirrer (gear 2) at 4°C for 6 hours.

[0045] (5) Clean and enhance nuclease enzyme activity: replace the submandibular gland tissue...

Embodiment 2

[0056] Embodiment 2, the making of rat submandibular gland ECM

[0057] (1) Material collection: select submandibular gland tissues of healthy rats, decompose them into small pieces of 1 cm square, and wash them twice with PBS. As for the EP tube, store it in a -80°C refrigerator for later use.

[0058] (2) Broken cells: Take out the tissue block from the -80°C refrigerator, thaw it in a 37°C water bath, and then put it back into the -80°C refrigerator for 30 minutes, and repeat the freezing and thawing process 3 times.

[0059] (3) Disinfection and preparation: Place the tissue block in 75% alcohol, suspend the submandibular gland tissue block with sutures, hang it in a 250ml jar, and place a 1cm magnetic stirring bar at the bottom of the bottle.

[0060] (4) Pre-cleaning and crushing: add 250ml of sterile third-grade water, and treat on a magnetic stirrer (gear 2) at 4°C for 12 hours.

[0061] (5) Clean and enhance nuclease enzyme activity: replace the submandibular gland ...

Embodiment 3

[0068] Example 3, the application of mouse submandibular gland ECM in the study of submandibular gland tissue remodeling

[0069] (1) Prepare cells: take two C57 mouse salivary glands and soak them in 75% ethanol for 15 seconds, rinse twice with pre-cooled 1×PBS, and cut them into 2mm pieces 3 sized pieces. Add 3ml dispase and digest in a 37°C water bath for 60min. Rinse twice with pre-cooled 1×PBS (centrifuge at 1000rpm for 4min, add 10ml 1×PBS and stir at 4°C for 10min). The submandibular gland cells were collected by filtration with a 40um filter membrane.

[0070] (2) Cell injection: the mouse submandibular gland ECM prepared in Example 1 was fixed on a new wax plate with a sterile pin in a clean bench. Use an alcohol lamp to pull the Pasteur tube into a glass needle with a diameter of about 100 μm, and draw the primary cells of the submandibular gland isolated in step (1) and inject them into the ECM tissue of the submandibular gland.

[0071] (2) Culture: Put the sub...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a decellularized submaxillary gland matrix material and a preparation method thereof. The preparation method is characterized in that: selected healthy animal submaxillary gland tissues are treated with a synthetic physical, chemical and enzymatic method, the treated tissues are soaked in different inorganic salt solutions, and treated in low-temperature environment to prepare the decellularized submaxillary gland matrix material with good biocompatibility and proper biodegradation rate. The material can be used in submaxillary gland regeneration and repair and used as a model for researching submaxillary gland diseases; the raw materials are wide in source, easy to get, low in price and easy to prepare.

Description

technical field [0001] The invention belongs to the processing field of biomedical materials, and in particular relates to a decellularized submandibular gland matrix material and a preparation method thereof. Background technique [0002] The extracellular matrix (ECM) is a protein and polysaccharide macromolecular substance secreted by cells into the extracellular mesenchyme. ECM constitutes a complex grid connection tissue structure through a highly organized extracellular microenvironment, and plays a key role in regulating cell physiological activities such as cell survival and differentiation. Therefore, scaffold substrates and scaffold materials that can mimic the ECM are ideal carriers for tissue engineering research and tissue repair. The contact between cells and ECM is dynamic, and changes in ECM will lead to changes in the morphology and function of neighboring cells. An ideal scaffold material for tissue engineering needs to provide the same or similar microen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50
Inventor 张雁赵龙吴愁
Owner SUN YAT SEN UNIV