Acrylic resin degrading bacteria and screening enrichment method thereof
A technology of acrylic resin and degrading bacteria, applied in the field of microbial screening, can solve the problems of corrosion, difficult control and high processing cost, and achieve the effects of improving tolerance and stability, improving degradation efficiency and mild reaction conditions
Inactive Publication Date: 2015-07-29
YANCHENG INST OF TECH
View PDF2 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, there are inevitably high operating costs, difficult control, and potential heavy metal pollution caused by the selection of heavy metal catalysts; supercritical water oxidation treatment of acrylic acid and its ester wastewater has the advantages of short reaction time, thorough removal of pollutants, Small footprint, clean and environmentally friendly, etc., is a physical and chemical treatment technology worthy of further development and utilization
However, equipment corrosion and inorganic salt deposition caused by high-concentration dissolved oxygen, high temperature and high pressure are the main problems faced by this technology; fiber adsorption method, Fenton oxidation method, photoelectron wave decomposition method to degrade acrylic acid and its ester wastewater have mild reaction conditions. , fast reaction speed, and high removal efficiency, but due to the lack of in-depth research and high processing costs, it has not yet been applied to the field of actual engineering processing
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreExamples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0032] (1) Collection of bacterial source and preparation of bacterial suspension: collect the soil at the sewage outlet of a Dongfeng Yueda Kia automobile paint factory, and store the sludge in a 4°C refrigerator for later use; put 20g of the collected soil in 250mL sterile Shake in water at 30°C for 12 hours to obtain a bacterial suspension;
[0033] (2) Take 5 mL of the bacterial suspension in step (1) and pour it into a Erlenmeyer flask containing 100 mL of inorganic salt medium, place it in a constant temperature shaker at 30°C, and shake it at 130r / min for 7 days to obtain the bacterial liquid;
[0034] (3) Inorganic salt medium: NaCl 0.5g, NH 4 NO 3 0.4g, KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, yeast extract powder 0.2g, trace element solution 4mL, distilled water 1000mL, adjust the pH value to 7.2; the composition of the trace element solution is: MgSO 4 2.0g, CuSO 4 0.5g, MnSO 4 0.5g, FeSO 4 ·7H 2 O 0.5g, CaCl 2 0.5g, distilled water 500mL;
[0035] (4) Take 1mL o...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention discloses acrylic resin degrading bacteria and a screening enrichment method thereof and belongs to the technical field of microorganism screening. The method comprises steps as follows: (1), preparation of a bacterium liquid; (2), preparation of acrylic resin degrading bacterial systems; (3), screening of the acrylic resin degrading bacterial systems; (4), secondary screening of purified degrading bacteria, wherein the step (2) is performed as follows: glucose is added to the bacterium liquid, acrylic acid wastewater is added, the mixture is inoculated to an inorganic salt culture medium, the mixture in a shaking bed is subjected to subculturing without light, the amount of glucose is reduced by half during each subculturing relative to the glucose used during previous subculturing, the amount of acrylic acid wastewater is increased relative to acrylic acid wastewater used during previous subculturing, each subculturing is performed for 7 d, more than five generations are generated through subculturing, and glucose is not added to the culture medium during culture of the last generation. Acrylic acid and ester wastewater thereof are treated with a biological method, the energy consumption is reduced, the reaction condition is mild, the efficiency is higher, and the acrylic resin degrading efficiency can reach 90% by the aid of degradation strains.
Description
technical field [0001] The invention belongs to the technical field of microbial screening, and in particular relates to an acrylic resin degrading bacterium and a screening and enrichment method thereof. Background technique [0002] Acrylic resin is an important chemical raw material widely used in paint, coating, leather, textile, printing and other industries. At present, the total output of acrylic resin in my country is about 1.2 million tons. According to the calculation of 1.2 tons of waste water per ton of acrylic acid produced, the total amount of waste water of acrylic acid and its esters in my country is about 1.4 million tons every year. Acrylic acid wastewater has complex components, high pollutant concentration, and strong environmental hazards, which make the treatment of acrylic acid and its ester wastewater very difficult. [0003] At present, the main treatment method of acrylic acid and its ester wastewater is incineration, which has the advantages of ma...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 钱晓荣董悦董锐唐帆
Owner YANCHENG INST OF TECH