Primers, probes, detection system and kit for one time detection of lung cancer multiple genes

Abstract

The present invention discloses primers, probes, a detection system and a kit for one time detection of lung cancer multiple genes, wherein the primers, the probes, and the distribution way for detecting 18 EGFR gene mutations, 7 KRAS gene mutations, BRAF V600E gene mutation, 5 fusion genes of ALK5, and 9 fusion genes of ROS1 are provided. According to the present invention, the detection kit adopts the 12 linking PCR reaction strip design, each 12 linking PCR strip detects multiple genes of a sample, the corresponding detection reagents and the internal control reagents are filled in the pipes 1-11 of the 12 linking PCR strip, the mutation is indicated by the FAM signal, and the internal control is indicated by the HEX (or VIC) signal; the pipe 12 is adopted as the DNA extraction quality external control detection pipe and is indicated by the FAM; and with the primers, the probes, the detection system and the kit, the one-time detection of the lung cancer EGFR / KRAS / BRAF / ALK / ROS1 gene can be achieved, such that the detection time is substantially shortened, the sensitivity is high, the specificity is strong, the operation is simple and rapid, and the reference for selection of tumor targeting drug therapy on lung cancer patients can be provided for clinician.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to primers, probes and a detection system for multiple genes of lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors with the highest morbidity and mortality among malignant tumors, among which non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancer. my country is the country with the highest incidence of lung cancer, and about 600,000 people are diagnosed with lung cancer every year. In the past 30 years, the mortality rate of lung cancer has increased by 464.84%. According to the World Health Organization (WHO), by 2025, the number of lung cancer deaths in my country will reach 1 million each year. At present, chemotherapy is still the most important treatment for lung cancer. Although with the emergence of a new generation of chemotherapy drugs, the efficacy of lung cancer has improved to a certain extent, but the overall...

AI Technical Summary

Problems solved by technology

However, at present, most of them can only detect one mutation or a mutation of a gene at a time, the detection throughput is low, and they can only be screened one by one, which takes a long time

At the same time, due to the difficulty in obtaining materials for advanced patients and the small sample size, they often cannot meet the needs of screening one by one, and there is no clinical kit that can detect these five hotspot genes at the same time and at one time.

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