Method for preparing plant growth regulator containing products by using low-value seaweeds

A technology of plant growth regulator and seaweed, applied in the direction of plant growth regulator, plant growth regulator, botany equipment and method, etc., can solve the problems of high preparation cost, heating energy consumption, complicated process, etc., and achieve easy operation , improve efficiency, simple process effect

Inactive Publication Date: 2015-08-12
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although many practitioners study the method of preparing plant growth regulator from seaweed, there are some problems in the existing methods: as the Chinese patent application number 201310063079.8 discloses "a preparation method of seaweed vegetative growth regulator ", the fresh seaweed or dried seaweed is crushed and homogenized after pretreatment, and the primary, secondary, and tertiary enzymatic hydrolysis are carried out with cellulase, papain, and pectinase respectively, and the enzymatic hydrolysis temperature of each stage is 50-55 ℃, enzymatic hydrolysis for 4-6 hours, after the enzymolysis is completed, the enzymolysis solution is filtered and concentrated; this method requires three-stage enzymolysis, the process is complicated, and the process requires heating and energy consumption, so the preparation cost is high and the efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a. After washing and drying the fresh sargassum, weigh 500 kg of dried sargassum and add 0.3 kg of BHT, then place it in a dark room at 4°C to pulverize and grind to obtain seaweed pulp; Add 90% ethanol to the slurry, and use 40 KHz ultrasonic assisted extraction for 1 h;

[0022] b, centrifuge the extract obtained in step a at 5000 rpm for 10 min, and collect the supernatant;

[0023] c. Add 90% ethanol to the seaweed residue obtained by centrifugation in step b at 2000 L / ton, and use 40 KHz ultrasonic assisted extraction for 1 h under dark conditions;

[0024] d, centrifuge the extract obtained in step c at 5000 rpm for 10 min, and collect the supernatant;

[0025] e. Add 90% ethanol to the seaweed residue obtained by centrifugation at 2000 L / ton in step d, and use 40 KHz ultrasonic assisted leaching for 1 h under dark conditions;

[0026] f, centrifuge the extract obtained in step e at 5000 rpm for 10 min, and collect the supernatant;

[0027] g. Combine the super...

Embodiment 2

[0031] a. After washing and drying the fresh Ascophyllum nodosum, weigh 1000 kg of dried Ascophyllum nodosum and add 0.5 kg BHQ, then place it in a dark room at 10°C to pulverize and grind to obtain seaweed pulp; press 2500 L / ton Add 95% ethanol to it, and use 20 KHz ultrasonic assisted extraction for 2 h;

[0032] b, centrifuge the extract obtained in step a at 6000 rpm for 5 min, and collect the supernatant;

[0033] c. Add 95% ethanol to the seaweed residue obtained by centrifugation in step b at 2500 L / ton, and use 20 KHz ultrasonic assisted extraction for 5 h under dark conditions;

[0034] d, centrifuge the extract obtained in step c at 6000 rpm for 5 min, and collect the supernatant;

[0035] e, the centrifuged seaweed residue gained in step d is added 95% ethanol to it at 2500 L / ton, and adopts 20 KHz ultrasonic assisted leaching for 5 h under dark conditions;

[0036] f, centrifuge the extract obtained in step e at 6000 rpm for 5 min, and collect the supernatant;

...

Embodiment 3

[0041] a. After washing and drying the fresh sage algae, weigh 1000kg of dried sage algae and add 0.5 kg of BHA, then place it in a dark room with a temperature of 6°C to grind and grind to obtain seaweed pulp; press 6000 L / Add 75% ethanol to it, adopt 80 KHz ultrasonic assisted leaching for 6 h;

[0042] b, centrifuge the extract obtained in step a at 4000 rpm for 15 min, and collect the supernatant;

[0043] c. Add 75% ethanol to the seaweed residue obtained by centrifugation in step b at 6000 L / ton, and use 80 KHz ultrasonic assisted extraction for 6 h under dark conditions;

[0044] d, centrifuge the extract obtained in step c at 4000 rpm for 15 min, and collect the supernatant;

[0045] e. Add 75% ethanol to the seaweed residue obtained by centrifugation at 6000 L / ton in step d, and use 80 KHz ultrasonic assisted extraction for 6 h under dark conditions;

[0046] f, centrifuge the extract obtained in step e at 4000 rpm for 15 min, and collect the supernatant;

[0047] g...

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PUM

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Abstract

The invention provides a method for preparing plant growth regulator containing products by using low-value seaweeds, which comprises the following steps: pretreating fresh seaweeds into which an antioxidant is added so as to obtain seaweed pulp, and leaching the seaweed pulp by using ethanol and being supplemented by ultrasonic waves; carrying out centrifugal separation on the obtained object so as to obtain leaching liquor and seaweed residues; continuing to repeatedly leach the seaweed residues by using 75-95% ethanol and being supplemented by ultrasonic waves twice and then carrying out centrifugal separation; and mixing supernatants, carrying out decompressed concentration on the obtained object so as to obtain a plant growth regulator containing concentrated solution, adding water into the concentrated solution, carrying out decompressed concentration on the obtained mixture so as to remove ethanol, and subpackaging the obtained product by using brown bottles, so that plant growth regulator containing products are obtained. The method disclosed by the invention is simple in process, easy to operate and high in efficiency, and the obtained products are more stable.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a product containing a plant growth regulator by using low-value seaweed. Background technique [0002] my country is rich in seaweed resources. Studies have shown that seaweed extracts contain plant growth regulators such as cytokinin, auxin, gibberellin, abscisic acid, ethylene, betaine, and polyamines. Plant growth regulators extracted from seaweed, It can promote the growth of plants and increase the yield of crops. At present, although many practitioners study the method of preparing plant growth regulator from seaweed, there are some problems in the existing methods: as the Chinese patent application number 201310063079.8 discloses "a preparation method of seaweed vegetative growth regulator ", the fresh seaweed or dried seaweed is crushed and homogenized after pretreatment, and the primary, secondary, and tertiary enzymatic hydrolysis are car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/03A01P21/00
Inventor 唐旭易勇方卫东徐长安刘源森林凌陈锋
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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