Mixing Tebuconazole and Nalidixic Acid for Sterilization in the Cultivation Process of Chlamydomonas reinhardtii

A technology of nalidixic acid and tebuconazole, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve problems such as contamination by bacteria, and achieve good preservation, good promotion and application value, and easy Promoted app performance
CN104830694BActive Publication Date: 2017-10-13XUZHOU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XUZHOU NORMAL UNIVERSITY
Publication Date
2017-10-13

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Abstract

The invention belongs to methods for killing bacteria and fungi in chlamydomonas reinhardtii and discloses a method for sterilization in a chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid. The method includes: A, inoculating chlamydomonas contaminated by mixed bacteria onto a TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in an occulting light period of 14 / 10 and under the light intensity of 8000Lx; C, transferring the chlamydomonas well grown on the TAP solid medium plate to a TAP plate with mixed antibacterial agents, and culturing for 5-8 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx; D, transferring the sterilized chlamydomonas to the common TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx to achieve a standby for subsequent experiments or breed conservation. The method has the advantage that the problem of bacterial contamination in scientific experiment and application process of chlamydomonas is solved by adoption of the mixed antibacterial agents for treating bacterial and fungus contamination of chlamydomonas.
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Description

technical field

[0001] The invention relates to a method for removing bacteria and fungi of Chlamydomonas reinhardtii, in particular to a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by mixing tebuconazole and nalidixic acid. Background technique

[0002] Chlamydomonas reinhardtii ( Chlamydomonas reinhardtii ) is a eukaryotic single-cell microalgae with short growth cycle, easy cultivation, annotated genome, mature related genetic biochemical technology and molecular biology technology, heavy metal adsorption capacity, and high oil production. As a model organism for scientific research and application, research on Chlamydomonas, a ciliated disease model, and research on biodiesel and biodecontamination have been carried out. However, in the process of scientific research and application, it is inevitable to cause the pollution of Chlamydomonas species during inoculation, and these miscellaneous bacteria pollution will endanger the pr...

Claims

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