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Preparation method and preparation apparatus of embedded immobilized microbial ball

A technology for immobilizing microorganisms and a preparation device, which is applied in the field of preparation methods for embedding and immobilized microorganism balls and preparation devices thereof, can solve the problems of short survival time, difficult oil separation and recovery, etc., and achieves easy ingestion, guaranteed survival rate, and size. High stability effect

Inactive Publication Date: 2015-08-12
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the technical problems of the short survival time of immobilized microorganisms in the prior art and the difficulty in separating and recovering oil after oil absorption by high oil-absorbing materials, the present invention provides a preparation method for embedding and immobilizing microorganism spheres. The buried immobilized microbial ball has a fast oil absorption rate and a large oil absorption capacity, and can degrade the absorbed oil by microorganisms, and the microorganisms have good stability in the carrier and a long survival time in the carrier, making it possible to effectively remove the oil in the water body for a long time. oil spill

Method used

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  • Preparation method and preparation apparatus of embedded immobilized microbial ball

Examples

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Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method for embedding immobilized microbial spheres, the specific steps are as follows:

[0046] (1) Preparation of immobilized microbial particles:

[0047] Take 80 mesh halloysite nanotubes and join in the citric acid solution that concentration is 3wt% and stir evenly, obtain acid activated halloysite nanotube liquid after acid activation 3h, the weight of described halloysite nanotubes and citric acid The dosage ratio is 1:3.

[0048] The acid-activated halloysite nanotube solution was thermally activated at 500° C., and was taken out after 1.5 hours to obtain thermally activated halloysite nanotubes. The modified halloysite nanotubes are obtained by pulverizing the thermally activated halloysite nanotubes through 200 mesh.

[0049] Take 10 parts of modified halloysite nanotubes and 60 parts of brevibacillus D-1 at a concentration of 6×10 9 The cell / g seed bacterial liquid is mixed until the modified halloysite nanotube absorbs the seed bacterial liq...

Embodiment 2

[0058] A preparation method for embedding immobilized microbial spheres, the specific steps are as follows:

[0059] (1) Preparation of immobilized microbial particles:

[0060] Take 60 mesh halloysite nanotubes and join in the citric acid solution that concentration is 3wt% and stir evenly, obtain acid activated halloysite nanotube liquid after acid activation 2h, the weight of described halloysite nanotubes and citric acid The dosage ratio is 1:4.

[0061] The acid-activated halloysite nanotube solution was thermally activated at 400° C., and was taken out after 2 hours to obtain thermally activated halloysite nanotubes. The modified halloysite nanotubes are obtained by pulverizing the thermally activated halloysite nanotubes through 150 mesh.

[0062] Take 10 parts of modified halloysite nanotubes and 40 parts of brevibacillus D-1 at a concentration of 6×10 9 The cell / g seed bacterial liquid is mixed until the modified halloysite nanotube absorbs the seed bacterial liqui...

Embodiment 3

[0071] A preparation method for embedding immobilized microbial spheres, the specific steps are as follows:

[0072] (1) Preparation of immobilized microbial particles:

[0073] Take 60 mesh halloysite nanotubes and join them in a citric acid solution with a concentration of 3wt% and stir evenly to obtain acid-activated halloysite nanotube liquid after acid activation for 1h. The weight of the halloysite nanotubes and citric acid The dosage ratio is 1:2.

[0074] The above acid-activated halloysite nanotube solution was thermally activated at 400° C., and was taken out after 1 hour to obtain thermally activated halloysite nanotubes. The modified halloysite nanotubes are obtained by pulverizing the thermally activated halloysite nanotubes through 200 mesh.

[0075] Take 10 parts of modified halloysite nanotubes and 30 parts of brevibacillus D-1 at a concentration of 6×10 9 The cell / g seed bacterial liquid is mixed until the modified halloysite nanotube absorbs the seed bacte...

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Abstract

The present invention relates to the field of high oil-absorbing resin preparation, and discloses a preparation method of an embedded immobilized microbial ball. The method comprises the steps of: (1) preparation of the immobilized microbial particles; (2) preparation of an acrylate copolymer emulsion; and (3) preparation of the embedded immobilized microbial ball. The present invention also discloses a preparation apparatus of the embedded immobilized microbial ball. The apparatus includes an extrusion section, an insulation transport section and a moulding section. The extrusion section comprises an extrusion sections housing, an advancing screw and a rotary motor; the insulation transport section includes a tubular type conveying channel and a heater; and the moulding section comprises a moulding section housing and a pair of reverse gears provided with hemispherical grooves. The embedded immobilized microbial ball prepared by the present invention has high oil suction rate and large oil absorption capacity, and can degrade the adsorbed oil; and microbes have good stability and long survival time. At the same time, the preparation apparatus of the present invention requires mild conditions in the preparation process, protects the survival rate of the microbes to the maximum; and the prepared product has high dimensional stability.

Description

technical field [0001] The invention relates to the field of embedding and immobilizing microorganisms and the field of preparation equipment for embedding and immobilizing microorganisms, in particular to a preparation method and a preparation device for embedding and immobilizing microorganism balls. Background technique [0002] In the past few decades, my country's industry has developed rapidly and the living standards of the people have been continuously improved. However, with the development of industry, the pollution of industry to the environment has become more and more serious. In recent years, people's awareness of environmental protection has gradually strengthened, and the government has paid more and more attention to environmental governance. [0003] Among the many sources of pollution, the problem of water pollution is particularly prominent. Among them, the pollution of oceans and rivers caused by blowouts, leaks from transport ships, sinking ships, and l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08C12M1/00C12R1/08
Inventor 陈庆国刘梅孙会玲孙静亚
Owner ZHEJIANG OCEAN UNIV
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