Preparation and application of core-shell type magnetic metal organic framework nano-particles

Abstract

The invention relates to preparation and an application of core-shell type magnetic metal organic framework nano-particles. The preparation comprises specific steps as follows: (1), a nano substrate with superparamagnetism is prepared; (2), carboxyl modified magnetic nano-particles are prepared; (3), 100-200 mg of carboxyl modified magnetic nano-particles prepared in the step (2) are dispersed in 5-10 mL ethanol solution containing 10 mmol L<-1> FeCl3.6H2O and are left to stand for 15-20 min at the room temperature, the obtained nano-particles are washed by ethanol for 2-3 times, and FeCl3*6H2O which is not adsorbed is removed; the magnetic nano-particles are dispersed in 5-10 mL ethanol solution containing 10 mmol L<-1> 1,3,5-trimesic acid and left to stand for 30-60 min at the temperature of 70 DEG C; the operation is performed circularly for 10-40 times, the obtained magnetic nano-particles are subjected to vacuum drying for 12-24 h at the temperature of 150 DEG C, and accordingly, the core-shell type magnetic metal organic framework nano-particles are obtained. By means of a pore structure and a large quantity of Fe<3+> ions, acting sites of a material and phosphoeptide are greatly increased, and selectivity, limit of detection and enrichment capacity of the material are improved.

Description

【Technical field】 [0001] The invention relates to the technical field of chemical industry, in particular to the preparation and application of core-shell magnetic metal organic framework nanoparticles. 【Background technique】 [0002] Reversible phosphorylation of proteins is one of the most important post-translational modifications of proteins, which regulates many physiological processes, such as cell division, cell proliferation, signal transduction and metabolism. It is estimated that 30% of proteins are phosphorylated at the same time, so the study of protein phosphorylation mechanism is very important for proteomics. Quantitative as well as qualitative detection of phosphorylated proteins is a prerequisite for understanding these processes. Today, mass spectrometry has proven to be an effective tool for the analysis and identification of protein phosphorylation sites. However, there are still many problems in directly analyzing samples by mass spectrometry. For exa...

AI Technical Summary

Problems solved by technology

However, few MOF materials with IMAC properties have been used for the enrichment of phosphopeptides so far.

Deng et al prepared a hydrophilic MOF material based on zirconium ions. This material combines the advantages of easy separation of magnetic spheres, the hydrophilicity of dopamine and the large specific surface area of ​​MOF materials. It is shown in the enrichment of phosphorylated proteins. High selectivity and enrichment efficiency, but the synthesis method is more complicated

Images