Solid-phase synthesis method for nano silicon dioxide particles based on histidine tag
A nano-silica and histidine-labeled technology, which is applied in the direction of silica, silicon oxide, nanotechnology for materials and surface science, etc., can solve the problems of difficult separation and purification, difficult sample recovery, etc., and achieve convenient purification , The effect of simplifying the preparation process and improving the utilization rate
Active Publication Date: 2015-09-02
SHANGHAI UNIV
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Abstract
The invention discloses a solid-phase synthesis method for nano silicon dioxide particles based on a histidine tag. The method comprises the following steps: firstly complexing with nickel ions on resin particle surfaces by virtue of the histidine tag of histidine tag protein; adsorbing the histidine tag protein on the resin particle surfaces; and growing the nano silicon dioxide particles employing immobilized protein as nucleus. The method has significant characteristics and advantages that nano particles are always kept in non-covalent link through the histidine tag and resin in the overall synthesis process of the nano silicon dioxide particles and the subsequent surface functionalization processes; the purification process after the reaction in each step is greatly simplified into a simple leaching process; the non-covalent link is reversible; after synthesis and functionalization are finished, the nano particles can be eluted from the resin particle surfaces though an eluant; and meanwhile, the eluted nano particles with the histidine tag can also be recovered through adsorption of the resin particles after being applied.
Application Domain
Material nanotechnologySilica
Technology Topic
Protein proteinImmobilized proteins +11
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PUM
Property | Measurement | Unit |
Particle size | 20.0 | nm |
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