Luminescent nano micro-spheres with positive charge on surface and possessing aggregation induced fluorescence enhancement property and biological application thereof

An aggregation-induced fluorescence and positively charged technology, which is applied in the fields of fluorescence/phosphorescence, nanotechnology, material excitation analysis, etc., can solve the problems of poor biocompatibility, short cycle time and ineffective entry into cells, and achieve low toxicity and excellent AIE property, low cytotoxic effect

Active Publication Date: 2015-09-09
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, AIE molecules are mostly small molecules of organic dyes, which have poor biocompatibility, shor

Method used

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  • Luminescent nano micro-spheres with positive charge on surface and possessing aggregation induced fluorescence enhancement property and biological application thereof
  • Luminescent nano micro-spheres with positive charge on surface and possessing aggregation induced fluorescence enhancement property and biological application thereof
  • Luminescent nano micro-spheres with positive charge on surface and possessing aggregation induced fluorescence enhancement property and biological application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.06g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The nano-microspheres obtained by polymerization were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted monomers, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain The mass concentration is 2.92% of the positively charged nanometer...

Embodiment 2

[0038] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.5g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The polymerized nanospheres were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain the mass concentration It is a 2.92% solution of nano-microspheres with positive charges on the su...

Embodiment 3

[0042] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.05g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The polymerized nanospheres were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain the mass concentration It is a 2.92% solution of nano-microspheres with positive charges on the s...

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Abstract

The invention relates to luminescent nano micro-spheres with positive charge on surface and possessing aggregation induced fluorescence enhancement property and a biological application thereof, which belongs to the technical field of high-molecular material. According to the invention, the nano micro-spheres with positive charge on surface is synthesized, the fluorescence molecules with positive charge and AIE effect are modified to the surface of the nano micro-spheres through electrostatic interactions. Molecule internal rotation of fluorescence molecule is restricted by Coulombian force effect, the absorbed energy can be basically released through fluorescence radiation, accordingly fluorescence from the fluorescence molecule modified to the nano micro-spheres can be enhanced by a hundred times, and excellent AIE property can be generated. The prepared the luminescent nano micro-spheres have the advantages of stable fluorescence property, good biological compatibility and low toxicity, and the positive charge on the surface is easily introduced to cell and biology detection. The luminescent nano micro-spheres with positive charge on surface has wide application prospect on the biology fields such as cell imaging.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a fluorescent nano-microsphere with a positive charge on the surface and an aggregation-induced fluorescence enhancement property and its application in cell imaging, biological detection and the like. Background technique [0002] Bioimaging is an emerging field that integrates multiple technologies, integrates multiple disciplines, has wide applications, and develops rapidly. In recent years, with the development of biochemistry and life sciences, people's research on organisms has gradually shifted from the macroscopic to the microscopic. Therefore, bioluminescent imaging, one of its branches, has become the focus of research and attention. Fluorescence technology has the advantages of fast, sensitive, real-time, non-radioactive, and good repeatability. Multiple photophysical parameters (such as emission wavelength, excitation wavelength, fluorescence in...

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F212/14C08F8/34G01N21/64B82Y40/00
Inventor 林权陈洁杨旭东杨雪孙源卿杨柏
Owner JILIN UNIV
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