Method for establishing mouse model with hepatic preneoplastic lesion caused by double factors of alcohol and HBV

A technology of precancerous lesions and mouse models, which is applied to the active ingredients of hydroxyl compounds, animal husbandry, etc., to achieve the effect of easy repeatability, low cost and simple production
CN104904660AActive Publication Date: 2015-09-16GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
Publication Date
2015-09-16

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Abstract

The invention relates to a method for establishing a mouse model with a hepatic preneoplastic lesion caused by double factors of alcohol and the HBV. The method includes the following steps that firstly, according to an animal, a high-duplication HBV transgenic mouse is copied in the substep of 1.3; secondly, white spirit serves as the reagent; thirdly, the mouse model with the hepatic preneoplastic lesion is prepared, the HBV transgenic mouse is fed with the white spirit every day, continuous gavage is conducted for 16-30 weeks, and a mouse eats and drinks freely during the period; fourthly, after-treatment of the model mouse with the hepatic preneoplastic lesion is conducted, wherein after gavage, weighing is conducted respectively, the mouse is taken and put to death, hepatic tissue is taken out, the hepar is weighed, the hepar index is calculated, and pathological section observation is conducted. The method has the advantages that the animal model is easy to manufacture, low in cost and easy to repeat; the HBV transgenic mouse model induced by alcohol for 22 weeks can be put under a scope so that it can be seen that atypical hyperplasia has large-cell and small-cell changes, the method conforms to the criteria of the hepatic preneoplastic lesion model, and successful model making is displayed and deduced.
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Description

field of invention

[0001] The invention relates to the field of animal models, in particular to a method for establishing a mouse model of liver cancer precancerous lesions caused by ethanol and HBV. Background technique

[0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary malignant tumors of the liver originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer, which is a malignant tumor with a high incidence in my country and is extremely harmful; rare. Secondary or metastatic liver cancer refers to malignant tumors originating from multiple organs throughout the body and invading the liver. Generally, it is more common in liver metastases of malignant tumors in stomach, biliary tract, pancreas, colorectum, ovary, uterus, lung, breast and other organs.

[0003] There are about 350 million people in the world who are chronic carriers of hepatitis B...

Claims

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