Sterilization composition containing ZJ5337 and methoxy acrylic ester sterilization agent
A technology of methoxyacrylic acid and ZJ5337, which is applied in the direction of fungicides, biocides, biocides, etc., can solve the problems that there are no related reports about the compounding of fenoxystrobin, and achieve slow resistance generation and good crop safety , good bactericidal effect
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Embodiment 1
[0028] Example 1, Indoor Toxicity Determination of ZJ5337 Compounded with Methoxyacrylate Fungicides to Rice Blast
[0029] Test object: Rice blast fungus (Pyricularia oryzae) was provided by the Institute of Biology, Zhejiang University.
[0030] Test method: Potted rice seedlings were sprayed and inoculated with spore suspension to determine the biological activity of the agent. The rice seedlings were cultivated until they had 2 leaves and 1 heart and were waiting for use. The test agent and the control agent were prepared into a drug solution with a preset concentration and sprayed. Each treatment was repeated 4 times. After 24 hours, take the culture dish full of spores, gently wash the fresh spores on the surface with sterile water, filter with double-layer gauze, and make a suspension with a concentration of 300,000 spores / ml, spray inoculation pressure (0.1MPa) . The inoculated test materials were moved to an artificial room (25°C), cultured in the dark at 100% humid...
Embodiment 2
[0052] Example 2, ZJ5337 combined with methoxyacrylate fungicides to determine the indoor toxicity of tomato early blight
[0053] Test object: Tomato early blight (Alternaria solai) was preserved in the strain room of this laboratory.
[0054] Test method: refer to the mycelium growth rate method in "Agricultural Industry Standard of the People's Republic of China NY / T1156.2-2006". Set 5 doses for each agent according to the content of active ingredients. Cultivate the above-mentioned pathogenic bacteria with PDA medium. When the colony is just full of 2 / 3 of the petri dish, use a puncher with a diameter of 5mm to form a bacterial block at the edge of the colony. , use an inoculation needle to move the bacterial block to the center of the pre-prepared toxic PDA medium, and then place it in a 25°C incubator for culture, and repeat 4 times for each treatment. Depending on the growth of the CK colony, use the cross method to measure the colony diameter cm of each treatment with...
Embodiment 3
[0058] Example 3, ZJ5337 and azoxystrobin, kresoxim-methyl compound pair pear scab indoor toxicity test
[0059] Test object: pear scab (Venturia pirina) is preserved in the strain room of this laboratory.
[0060] Test method: adopt the germ tube length method, set 5 doses for each drug according to the active ingredient content, and make a drug-containing tablet. Take 20 microliters of spore suspension, spread evenly on the drug-containing plate; incubate at 22°C in the dark for 10-12 hours, and then use a microscope to investigate the relative length of the spore germ tube. Investigate 5 fields of view for each concentration, and 30-40 spores per field of view. Germ tube length greater than or equal to 50% of the short diameter of the spore was considered normal germination. Taking the length of the short diameter of the spore as the unit "1", the relative length of the germ tube is the ratio of the actual length of the germ tube to the length of the short diameter of the...
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