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Method for extracting amino acids in worm grass

An amino acid, Cordyceps technology, applied in organic chemistry methods, chemical instruments and methods, purification/separation/stabilization of organic compounds, etc., can solve problems such as unfavorable industrial applications and complex processes

Inactive Publication Date: 2015-09-16
吴爱群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is complicated and not conducive to industrial application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Pre-treatment of Cordyceps sinensis: After washing and removing impurities, the Cordyceps sinensis is dried in the sun.

[0023] Step 1: Crush 2g of Cordyceps sinensis into 100 meshes, add 7g of 50°C water and Cordyceps sinensis into a 50ml Erlenmeyer flask, place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 3 times, and collect the aqueous solutions of the 3 experiments.

[0024] Step 2: Dry the Cordyceps sinensis after water extraction and put it into a 50ml Erlenmeyer flask, then add 4.6g ethanol; place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 5 times, and collect the supernatant; Drop off the ethanol in the supernatant.

[0025] Step 3: Use a straw to absorb the concentrated solution obtained in step 2, and slowly add the concentrated solution along the inner wall of the chromatography column through the straw. After the sample enters the column, wash it with ethyl acetate solution...

example 2

[0027] Pre-treatment of Cordyceps sinensis: After washing and removing impurities, the Cordyceps sinensis is dried in the sun.

[0028] Step 1: Crush 2g of Cordyceps sinensis into 100 meshes, add 7g of 50°C water and Cordyceps sinensis into a 50ml Erlenmeyer flask, place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 3 times, and collect the aqueous solutions of the 3 experiments.

[0029] Step 2: Dry the Cordyceps sinensis after water extraction and put it into a 50ml Erlenmeyer flask, then add 4.6g ethanol; place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 5 times, and collect the supernatant; Drop off the ethanol in the supernatant.

[0030] Step 3: Use a straw to absorb the concentrated solution obtained in step 2, and slowly add the concentrated solution along the inner wall of the chromatography column through the straw. After the sample enters the column, wash it with ethyl acetate solution...

example 3

[0032] Pre-treatment of Cordyceps sinensis: After washing and removing impurities, the Cordyceps sinensis is dried in the sun.

[0033] Step 1: Crush 2g of Cordyceps sinensis into 100 meshes, add 7g of 50°C water and Cordyceps sinensis into a 50ml Erlenmeyer flask, place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 3 times, and collect the aqueous solutions of the 3 experiments.

[0034] Step 2: Dry the Cordyceps sinensis after water extraction and put it into a 50ml Erlenmeyer flask, then add 4.6g ethanol; place the Erlenmeyer flask in a shaker for extraction for 10 minutes, repeat the experiment 5 times, and collect the supernatant; Drop off the ethanol in the supernatant.

[0035] Step 3: Use a straw to absorb the concentrated solution obtained in step 2, and slowly add the concentrated solution along the inner wall of the chromatography column through the straw. After the sample enters the column, wash it with ethyl acetate solution...

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Abstract

The invention provides a method for extracting amino acids in worm grass, which comprises the following steps: pulverizing worm grass into 100 meshes, putting the worm grass into a conical flask, putting the conical flask in an oscillator, and extracting with 35-65-DEG C water 3 times, wherein the mass ratio of the worm grass to the water in each water extraction process is 1:(3-5); drying the worm grass subjected to water extraction, putting into a conical flask, putting the conical flask in an oscillator, extracting with a solvent 3-8 times, collecting the supernate, and rectifying to remove the solvent in the supernate, wherein the mass ratio of the worm grass to the alcohol in each alcohol extraction process is 1:(1-4); and sucking the concentrated solution with a suction tube, slowly adding the suction tube into the concentrated solution along the inner wall of a chromatographic column, after the sample enters the column, flushing with an ethyl acetate solution in which the acetone concentration is 1-6%, and respectively collecting effluent liquids of all layers on the bottom of the chromatographic column. The method for extracting amino acids in worm grass has the advantages of simple technique, favorable separating effect and the like, and is easy to implement.

Description

technical field [0001] The invention relates to a method for extracting amino acids from Cordyceps sinensis. More specifically, the present invention relates to a method for extracting amino acids by column chromatography. Background technique [0002] Cordyceps sinensis is a precious Chinese herbal medicine in my country. It belongs to medicinal fungi. It is a fungus complex formed by parasitic fungi on insects and their parasitic insects. Studies in recent years have shown that Cordyceps sinensis has various pharmacological effects such as calming down, inhibiting bacteria, inhibiting cancer, and enhancing the body's immune function. [0003] Cordyceps sinensis has a variety of active ingredients, which have a wide range of pharmacological effects. Cordyceps polysaccharides can enhance immunity, anti-tumor and hypoglycemic effects; D-mannitol; fat and fatty acids, 12 kinds of fatty acids have been found so far; antibacterial active ingredients; alkaloids, their content i...

Claims

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Application Information

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IPC IPC(8): C07B63/00
Inventor 吴爱群
Owner 吴爱群
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