Flow cytometry fluid path system and flow cytometry method

A technology of flow cytometry and detection solution, which is applied in the direction of measuring devices, individual particle analysis, scientific instruments, etc., can solve the problems of high cost, high overall cost, and complex system, and achieve low cost, simple detection method, and accurate detection data Effect

Inactive Publication Date: 2015-09-16
GUANGZHOU EXCBIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The first type of constant pressure air source needs to be equipped with a set of air source and its pressure stabilizing device, the system is complex and the cost is relatively high
In the second type, each sy...

Method used

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  • Flow cytometry fluid path system and flow cytometry method
  • Flow cytometry fluid path system and flow cytometry method
  • Flow cytometry fluid path system and flow cytometry method

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Embodiment

[0033] Example: such as figure 1 , figure 2 , image 3 As shown, the fluid circuit system for flow cytometry detection of the present invention includes: a sample flow injector 2, a sheath fluid injector 3, a sampling injector 18, a first hemolytic agent injector 13, a second hemolytic agent injector 20, a sampling needle 19, a reaction pool 11, Flow chamber 8, diluent bucket 12, first hemolytic agent bottle 15, second hemolytic agent bottle 21, motor 1, motor 16, motor 14, two-way solenoid valve V1, two-way solenoid valve V2, two-way solenoid valve V3, Two-way solenoid valve V4, three-way solenoid valve V5, three-way solenoid valve V6, three-way solenoid valve V7, pinch valve PV1, liquid pressure sensor LP, three-way joint 4, three-way joint 5, and three-way joint 10. The flow chamber 8 is provided with a sheath flow inlet 6, a sample flow inlet 7 and a flow chamber waste outlet 9; the flow chamber waste outlet 9 is connected to the two-way solenoid valve V4; the outlet of...

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Abstract

The invention relates to the technical field of human blood detection and discloses a flow cytometry fluid path system. The system consists of a motor drive sample stream injector and a sheath fluid injector, wherein the outlet of the sample stream injector is respectively connected with a diluent bucket and a sample injection port by virtue of a pipeline and a three-way solenoid valve and is also connected with a reaction tank; the outlet of the sheath fluid injector respectively passes through a two-way solenoid valve by virtue of a pipeline and a liquid pressure sensor and is connected with a flow chamber sample injection port, a flow chamber sheath fluid injection port and a fluid suction port of the sample stream injector; a hemolytic agent injector is driven by the motor, and the outlet of the hemolytic agent injector is respectively connected with a hemolytic agent bottle and the reaction tank by virtue of a pipeline and a three-way solenoid valve; the outlet of a sampling injector is connected with a sampling needle by virtue of a pipeline; and a diluent injection port in the sampling injector is connected with the diluent bucket by virtue of a pipeline. The system disclosed by the invention is simple in structure, low in cost and simple and reliable in detection method, the detection data is accurate, and machine miniaturization can be realized.

Description

technical field [0001] The invention relates to the technical field of human blood detection, in particular to a flow cytometry detection fluid circuit system and a flow cytometry detection method. Background technique [0002] Sheath flow technology, also known as flow cytometry, uses a capillary to align with a small hole in the detection tube, and the cells or particles to be tested are ejected from the capillary to form a sample flow. At the same time, the sheath fluid (a clean liquid that does not interfere with the test results) flowing out of the capillary wraps the sample flow and flows quickly through the detection area, and ensures that the cells or particles are arranged in a single line in the middle of the detection area. The fluid circuit system for flow cytometry is a fluid system applied to medical blood cell analysis instruments, and it is the control carrier for flow cytometry. Flow cytometry refers to the fluid control technology that controls cells or pa...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 谭玉华秦军芳张炎梁铁柱
Owner GUANGZHOU EXCBIO TECH CO LTD
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