Bran protein feed preparing method by using mixed bacteria multi-step fermentation

A protein feed and bran technology, applied in animal feed, animal feed, application and other directions, can solve the problems of little deep processing and reuse, low economic value, etc., to improve the nutritional value of feed, good palatability, bran The effect of increasing protein content

Inactive Publication Date: 2015-09-23
QINGDAO JIARUI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our country is rich in wheat bran resources. The annual processing of bran in the world is 200-300 million tons, and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing bran protein feed through multi-step fermentation of mixed bacteria, the specific preparation steps are as follows:

[0025] (1) Pretreatment: select bran without mildew, crush it through a 40-mesh sieve, and adjust the water content of the bran to about 16%;

[0026] (2) Enzymolysis: add 7% α-amylase, 2.5% protease, 4% cellulase, and 2.5% xylanase in the mass ratio of bran, mix well, enzymolysis for 9 hours, and control the enzymolysis temperature at 40~ 50°C;

[0027] (3) Preparation of solid medium: add bran mass ratio of 6.5% molasses, 2.5% corn steep liquor, 2.5% ammonium nitrate, 1.2% ammonium sulfate, 0.15% potassium dihydrogen phosphate to the enzymatically hydrolyzed bran to adjust the medium Water content 30-40%;

[0028] (4) Aspergillus activation: take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.2, mix well until there is no dry powder and no agglomeration. After mixing ...

Embodiment 2

[0037] A method for preparing bran protein feed through multi-step fermentation of mixed bacteria, the specific preparation steps are as follows:

[0038] (1) Pretreatment: select bran without mildew, crush it through a 40-mesh sieve, and adjust the water content of the bran to 18%;

[0039] (2) Enzymolysis: Add 8% α-amylase, 3% protease, 5% cellulase, and 3% xylanase in the mass ratio of bran, mix well, enzymolysis for 6 hours, and control the enzymolysis temperature at 40~ 50°C;

[0040] (3) Preparation of solid medium: add bran mass ratio of 8% molasses, 3% corn steep liquor, 3% ammonium nitrate, 1.5% ammonium sulfate, 0.2% potassium dihydrogen phosphate to the enzymatically hydrolyzed bran to adjust the medium Water content 30-40%;

[0041] (4) Aspergillus activation: Take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of bran:water=1:1.3, mix well until there is no dry powder and no agglomeration. After mixing well, divide into 200...

Embodiment 3

[0050] A method for preparing bran protein feed through multi-step fermentation of mixed bacteria, the specific preparation steps are as follows:

[0051] (1) Pretreatment: select bran without mildew, crush it through a 40-mesh sieve, and adjust the water content of the bran to 12%;

[0052] (2) Enzymolysis: add 5% α-amylase, 2% protease, 3% cellulase, and 2% xylanase in the mass ratio of bran, mix well, enzymolysis for 12 hours, and control the enzymolysis temperature at 40~ 50°C;

[0053] (3) Preparation of solid medium: add bran mass ratio of 5% molasses, 2% corn steep liquor, 2% ammonium nitrate, 1% ammonium sulfate, 0.1% potassium dihydrogen phosphate to the enzymatically hydrolyzed bran to adjust the medium Water content 30-40%;

[0054] (4) Aspergillus activation: take fresh bran, sieve fine powder with a 60-mesh sieve, add water according to the ratio of bran: water = 1: 1.0, mix well until there is no dry powder and no agglomeration. After mixing well, divide into ...

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PUM

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Abstract

The invention discloses a bran protein feed preparing method by using mixed bacteria multi-step fermentation. A microbial fermentation technology is utilized, and by means of a 'composite enzymatic hydrolysis and multi-bacteria three-step solid state fermentation' technology, bran protein feed which is high in nutritive value and good in palatability is prepared, high value of the nutritive value for the bran feed is achieved, a product is golden yellow, slight lactic acid taste is obtained, the palatability is good, after the fermentation is conducted, the crude protein content is raised to 14.4% to 19.05%, nutritional ingredients such as profitable viable bacteria, various vitamins and enzymes are contained abundantly, and the method has the advantages that the growth and the development are promoted, the use ratio of the feed is improved, the immunity is enhanced, the intestinal microecology is improved, and diseases are prevented from generating. The method adopts the solid state fermentation, the production technology is simple, the energy consumption is low, the method is free of environmental pollution, the investment is small, and large-scale production is facilitated.

Description

technical field [0001] The invention relates to a method for preparing bran protein feed through multi-step fermentation of mixed bacteria, belonging to the field of feed processing. Background technique [0002] Protein raw materials are the foundation of the development of feed industry. The shortage and high price of protein raw materials in my country have become the bottleneck restricting the development of animal husbandry. At present, with the improvement of people's living standards and the emphasis on the quality of life, the demand for meat, eggs, milk, and aquatic products is increasing day by day. The production of grain and feed is facing severe challenges, and feed protein resources will become increasingly scarce. It is imminent to expand feed resources and develop unconventional feed. The important strategy to alleviate the contradiction is to improve the utilization rate of the existing protein source, tap the available nutritional components of the feed as...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K1/165A23K1/00
Inventor 侯文燕董静静代树强
Owner QINGDAO JIARUI BIOLOGICAL TECH
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