Microbial active filler containing paenibacillus sp., and application thereof

A technology of microbial activity and bacillus, applied in the field of environmental governance, can solve the problems of slow startup, easy shedding of load, poor acid and high temperature resistance, etc., and achieve the effect of short adaptation period, not easy to rot and deform, and not easy to lose

Active Publication Date: 2015-09-23
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problems in the prior art that the loads of microorganisms on the surface of the carrier are easy to fall off, start slowly, and have poor acid and

Method used

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  • Microbial active filler containing paenibacillus sp., and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1: Identification of strains

[0045] Use the automatic magnetic bead nucleic acid extractor Smart LabAssist-16 and the corresponding environmental sample DNA extraction and purification kit (Taiwan Dot Nanotechnology Co., Ltd.) to screen the strain DNA for extraction and purification, and use the bacterial universal primer F16S-27 And R16S-1492 for PCR amplification, the primer sequences are:

[0046] F16S-27(5`-AGAGTTTGATCCTGGCTCAG-3`)

[0047] R16S-1492(5`-CGGTTACCTTGTTACGACTTC-3`)

[0048] The PCR reaction conditions were set as follows: pre-denaturation at 94°C for 5min; subsequent denaturation at 94°C for 30s, annealing at 56°C for 30s, extension at 72°C for 90s, and cycling for 25 cycles; then extension at 72°C for 10min; and finally at 4°C for 10min. Then the PCR product was ligated to the PMD-19T vector (Dalian Bao Biological Co., Ltd.), and then transformed into E. coli DH5α competent cells (Dalian Bao Biological Co., Ltd.). 50 μl of transformed competent ce...

Example Embodiment

[0049] Example 2: A microbial filler for removing sulfur dioxide gas and its preparation

[0050] A microbial active filler for removing sulfur dioxide-containing gas, the filler is attached with a suspension of Paenibacillus sp. IB1, the Paenibacillus sp. IB1, the preservation number is CGMCC No. 10775, and the filler is polyurethane Foam block

[0051] The packing density is 15kg / m 3 , The porosity is 90%, and the particle size is 20mm;

[0052] The Paenibacillus IB1 is attached to the filler in the form of a bacterial suspension;

[0053] The mass ratio of the Paenibacillus IB1 suspension to the filler is 60:10;

[0054] The concentration of Bacillus IB1 cells in the microbial active filler is 1.1×10 6 CFU / g;

[0055] The preparation steps of the microbial active filler are as follows:

[0056] (1) Slant culture: prepare Paenibacillus sp. IB1 slant strain;

[0057] The composition of the Bacillus sp. IB1 slant medium is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2.0g, NH 4 Cl 0...

Example Embodiment

[0066] Example 3: A microbial filler for removing sulfur dioxide gas and its preparation

[0067] A microbial active filler for removing sulfur dioxide-containing gas, the filler is attached with a suspension of Paenibacillus sp. IB1, the Paenibacillus sp. IB1, the preservation number is CGMCC No. 10775, and the filler is volcanic rock ;

[0068] The packing density is 25kg / m 3 , The porosity is 55%, and the particle size is 10mm;

[0069] The Paenibacillus IB1 is attached to the filler in the form of a bacterial suspension;

[0070] The mass ratio of the Paenibacillus IB1 bacterial suspension to the filler is 36:10;

[0071] The concentration of Bacillus IB1 cells in the microbial active filler is 3.5×10 6 CFU / g;

[0072] The preparation steps of the microbial active filler are as follows:

[0073] (1) Slant culture: prepare Paenibacillus sp. IB1 slant strain;

[0074] The composition of the Paenibacillus IB1 slant medium is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2.0g, NH 4 C...

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Abstract

The invention discloses a microbial active filler containing paenibacillus sp., and an application thereof in removing sulfur-dioxide-containing gas. Specifically, the strain is paenibacillus sp. IB1 with a preservation number of CGMCC No. 10775. The microbial active filler prepared by loading a bacterial suspension liquid of the strain on a porous filling material can be used for treating sulfur-dioxide-containing gas, wherein a sulfur dioxide degradation efficiency reaches 90-100%. The microbial active filler has the characteristics of short start-up period and high loading capacity. The paenibacillus sp. IB1 has high acid resistance, and can be used for continuously and effectively purifying sulfur dioxide in a strongly acidic (pH2) environment. The paenibacillus sp. IB1 can be adapted to a high-temperature condition. Under a temperature of 50-65 DEG C, paenibacillus sp. IB1 can grow well and can effectively remove sulfur dioxide in waste gas with relatively high temperatures.

Description

Technical field: [0001] The invention belongs to the field of environmental treatment, and in particular relates to a microbial active filler containing Paenibacillus and its application in removing waste gas containing sulfur dioxide. Background technique: [0002] Coal combustion, metal smelting, flue gas acid production, sludge indirect drying and other processes discharge a large amount of waste gas containing sulfur dioxide. Excessive levels of sulfur dioxide in the atmosphere not only have a serious impact on the ecological environment, but also have great harm to human health. In addition, the acid rain formed by the emission of sulfur dioxide into the atmosphere will cause great harm to construction equipment. All countries in the world have strict restrictions on the emission of sulfur dioxide, and my country's regulations on sulfur dioxide emission are becoming more and more stringent. [0003] Compared with other physical and chemical waste gas treatment methods...

Claims

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Application Information

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IPC IPC(8): B01D53/85B01D53/50
CPCY02A50/20
Inventor 李琳林坚刘俊新
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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