Preparation method of anabasine

A technology of anathanaine and anabenaline, which is applied in the field of preparation of anabenaline, can solve problems such as rare anabenaline, and achieve the effects of continuous preparation, small amount of solvent, and simple and easy-to-operate method.

Inactive Publication Date: 2015-09-30
NANJING ZELANG MEDICAL TECH
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[0005] At present, there are few patent reports

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  • Preparation method of anabasine

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Example Embodiment

[0019] Example 1:

[0020] Take the dried quinoa squash and pass it through a 20-mesh sieve; weigh a certain amount of coarse powder, add a weight-volume ratio of 1:2, 70% ethanol, and add 0.1%% of the coarse powder weight of pectinase at the pH value 4. Soak the enzymatic hydrolysis at a temperature of 45℃ for 1 hour; after the enzymatic hydrolysis is completed, the obtained enzymatic hydrolysate is subjected to ultrasonic extraction at a frequency of 20KHz and a temperature of 50℃ for 1 hour, filtered, collected the filtrate, and the filter residue is extracted once again. ; Collect the extract and add the hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 2000 to ultrafiltration, and then concentrate the permeate with a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 100. Collect the concentrated solution and add 1 / 3 of the volume of the concentrated solution. Stir 1 / 4 and 1 / 6 of chloroform, stand for layering, collect the ...

Example Embodiment

[0021] Example 2:

[0022] Take the dried quinoa squash and pass through a 60-mesh sieve; weigh a certain amount of coarse powder, add weight to volume ratio of 1:4, 75% ethanol soak, and add 0.3% of the weight of coarse powder cellulase at pH 5 Soak the enzymatic hydrolysis at a temperature of 45℃ for 2h; after the enzymatic hydrolysis, the obtained enzymatic hydrolysate is subjected to ultrasonic extraction for 1.5h at a frequency of 30KHz and a temperature of 60℃, filtered, collected the filtrate, and the filter residue is extracted twice ; Collect the extract and add the hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 6000 to ultrafiltration, and then concentrate the permeate with a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 300. Collect the concentrated solution and add 1 / 3 of the concentrated solution volume. Stir 1 / 4 and 1 / 6 of chloroform, stand for layering, collect the lower layer, and combine the extracts; th...

Example Embodiment

[0023] Example 3:

[0024] Take the dried quinoa squash and pass through an 80 mesh sieve; weigh a certain amount of coarse powder, add a weight-volume ratio of 1:5, 80% ethanol soak, and add 0.5% hemicellulase by weight of the coarse powder at pH 5. Soak the enzymatic hydrolysis at a temperature of 50℃ for 3h; after enzymatic hydrolysis, the obtained enzymatic hydrolysate will be subjected to ultrasonic extraction at a frequency of 40KHz and a temperature of 70℃ for 2h, filter, collect the filtrate, and repeat the extraction twice for the filter residue ; Collect the extract and add it to a hollow cellulose ultrafiltration membrane with a molecular weight cut-off of 10,000. The permeate is then concentrated with a hollow cellulose nanofiltration membrane with a molecular weight cut-off of 500. Collect the concentrated solution and add the concentrated solution volume to 1 / 3, Stir 1 / 4 and 1 / 6 of chloroform, stand for layering, collect the lower layer, and combine the extracts; th...

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Abstract

The invention discloses a preparation method of anabasine. The preparation method comprises the following steps: raw material treatment, enzymolysis, ethanol ultrasonic extraction, purification, refinement and freeze-drying to obtain the anabasine. The method has the advantages of high efficiency and high speed, and is suitable for producing high-purity anabasine.

Description

technical field [0001] The invention relates to a preparation method of anabasine, which belongs to the field of biotechnology. Background technique [0002] Anabasine, English name: anabasine, also known as neonicotinoid; CAS number: 13078-04-1; molecular formula C 10 h 14 N 2 ; Molecular weight: 162.23; Exists in An-abasis apylla (An-abasis apylla). Boiling point 276°C; soluble in water, ethanol, ether and benzene, density (20°C) 1.0445g / cm3, refractive index 1.5430, optical rotation -82.20°, can be used as an insecticide. The molecular structural formula is as follows: [0003] [0004] High-speed countercurrent chromatography technology is a liquid and liquid chromatography technology that does not use any homogeneous carrier. Its principle is based on the relative movement of components in the rotating spiral tube and the separation between two immiscible two-phase solvents. And the speed is comparable to HPLC. HSCCC has high separation efficiency, high product...

Claims

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Application Information

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IPC IPC(8): C12P17/16
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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