Application of gardenia series pigments to bacteriostasis

A technology of gardenia series and gardenia blue pigment, which is applied in the field of food additives, can solve problems such as the limitation of the application range of natural pigments, and achieve the effects of avoiding poisonous problems, good antibacterial function, and soft and harmonious colors

Active Publication Date: 2015-10-07
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, in the process of using natural pigments, coloring is the

Method used

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  • Application of gardenia series pigments to bacteriostasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The preparation of embodiment 1 gardenia series pigment

[0016] (1) Preparation of Gardenia Yellow Pigment

[0017] The gardenia fruit was pulverized through a 40-mesh sieve, and the obtained gardenia powder was extracted with deionized water at a mass ratio of 1:10 at 45° C. for 1 hour to obtain a crude extract of gardenia yellow pigment.

[0018] Refining of gardenia yellow pigment: Adsorb gardenia yellow pigment with AB-8 macroporous adsorption resin, add 30g wet resin to the triangular flask containing 100 mL of crude extract of gardenia yellow pigment, put it into a shaker for adsorption for more than 10 hours , and then filter the resin and solution in the triangular flask together, collect the resin and then pack it into a column. Rinse with 4BV (the bottom area of ​​the separation column is 2.0096 square centimeters multiplied by the height of the resin in the separation column, 14.5 centimeters is 1BV) water, 5BV 20% ethanol, 6.5BV 60% ethanol and collect eac...

Embodiment 2

[0029] The antibacterial experiment of embodiment 2 gardenia series pigments

[0030] Operate under a sterile workbench, cool the prepared beef extract peptone medium to about 60°C and pour it into a sterilized petri dish, and use a sterile pipette to absorb the concentration of 10 6 200 μL of cfu / mL Bacillus subtilis or Proteus bacterial solution was evenly spread on the surface of the plate with a sterile glass triangular coating stick, and it was ready for use. Place 6 sterile Oxford cups on each plate (the inner diameter of the Oxford cup is 6 mm and the outer diameter is 7 mm), one sterile Oxford cup in the middle is the blank control, and the remaining 5 Oxford cups are the sample group. Add 100 μL of sterile water to the blank control, and add 100 μL of an aqueous solution of gardenia red pigment, gardenia yellow pigment or gardenia blue pigment with a concentration of 20 μg / mL filter sterilized to the sample group. Take it out, and then incubate at 37°C for 24 hours, ...

Embodiment 3

[0034] The minimum inhibitory concentration (MIC) experiment of embodiment 3 gardenia series pigments

[0035] Operate under a sterile workbench, cool the prepared sterilized beef extract peptone medium to about 60°C and pour it into a sterilized petri dish, and use a sterile pipette to absorb the concentration of 10 6 200 μL of cfu / mL Bacillus subtilis or Proteus bacterial solution was evenly spread on the surface of the plate with a sterile glass triangular coating stick, and it was ready for use.

[0036] Put 6 sterile Oxford cups on each plate (the inner diameter of the Oxford cup is 6mm, the outer diameter is 7mm), the middle 1 sterile Oxford cup is the blank control, the remaining 5 Oxford cups are the sample group, and the blank control is added with 100 μL sterile Water, 5 Oxford cups numbered 1, 2, 3, 4, and 5 in the sample group were added with 100 μL of filter-sterilized gardenia at concentrations of 20 μg / mL, 15 μg / mL, 10 μg / mL, 5 μg / mL, and 1 μg / mL. The aqueous s...

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Abstract

The invention discloses an application of gardenia series pigments to bacteriostasis, which belongs to the field of food additives. According to the application, bacteriostasis experiments show that gardenia yellow pigments, gardenia red pigments and gardenia blue pigments can inhibit the growth of gram-positive bacteria and gram-negative bacteria, such as bacillus subtilis and proteus. On the basis of the function of bacteriostasis, the natural gardenia series pigments with three color primaries of red, yellow and green are food additives capable of adjusting any color, have the effects of bacteriostasis and corrosion prevention, can be used for coloring and prolonging the expiration date of food, can be used for preparing bacteriostatic agents or preservatives and have good application prospects.

Description

technical field [0001] The invention belongs to the field of food additives, in particular to the application of gardenia series pigments in antibacterial. Background technique [0002] Currently in the food processing industry, the use of synthetic pigments is very common. Synthetic pigments refer to organic pigments obtained by artificial chemical synthesis, mainly using aniline dyes separated from coal tar as raw materials. Most synthetic pigments cannot provide nutrients to the human body, and some even have serious toxicity, teratogenicity and carcinogenicity to the human body. In addition to being toxic by themselves or their metabolites, many synthetic edible pigments may also be mixed with heavy metals such as arsenic and lead in the production process. Due to the stable nature of heavy metal elements, they are not easily decomposed or excreted by the human body, and are easily precipitated in the body. Long-term accumulation will lead to The occurrence of diseases...

Claims

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Application Information

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IPC IPC(8): A23L3/3463
CPCA23L3/3463
Inventor 方尚玲陈茂彬曹敬华严锦孟鑫周敬波
Owner HUBEI UNIV OF TECH
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