Washing-free cleansing oil having repairing effect
A technology of repairing and cleansing oil, applied in cosmetics, cosmetics, cosmetic preparations, etc., can solve the problems of people with sensitive skin, skin sensitivity, skin damage, and rising production and processing costs, so as to improve the relative growth rate , block burning and tingling, and reduce stickiness
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Embodiment 1
[0036] The preparation of embodiment 1 cleansing oil
[0037] The formula is shown in Table 1:
[0038] Table 1 Cleansing Oil Formula
[0039]
[0040] Sacha Sacha Sacha oil, olive oil and lecithin are mixed uniformly according to the above formula at normal temperature, then tea polyphenols, vitamin E and vitamin C are added according to the above formula, and after continuing to stir evenly at normal temperature, the cleansing oil is prepared.
Embodiment 2
[0041] Example 2 Protection of Sensitive Skin Verification Test
[0042] 1. Test principle Sensitive skin is usually accompanied by excessive reaction of peripheral nerves, especially the burning and tingling sensation caused by overactive TRPV1. Studies have shown that the sensitivity of TRPV1 receptors in peripheral nerves to capsaicin is significantly correlated with self-reported sensitive skin phenomena. The sample is used as a specific TRPV1 antagonist, and human tests have proved that it can effectively block the burning and tingling sensation caused by capsaicin, so as to effectively relieve skin discomfort.
[0043] 2. Test scheme In vivo test scheme: 30 volunteers carry out research test to the sensitivity of capsaicin, use two kinds of different ointments to be applied to the face, one kind contains capsaicin, one kind contains capsaicin and 0.4% sample (sample They are respectively: the cleansing oil of culture 1, the cleansing oil of formula 6, and olive oil) fol...
Embodiment 3
[0047] Example 3 Protection of Sensitive Skin Verification Test
[0048] The MTT method is used to detect the effect of functional substances on the proliferation of human fibroblasts in vitro (this method can be applied to the pre-screening and verification process of the development of cosmetic functional substances to detect cell survival and growth, and has high sensitivity and can be screened in large quantities. Compared with Animal experiments are more economically feasible)
[0049] 1. MTT principle
[0050] 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenyltetrazoliumromide, or MTT, is a yellow dye. Succinate dehydrogenase in the mitochondria of living cells can metabolize and reduce MTT, and at the same time, under the action of cytochrome C, produce blue (or blue-purple) insoluble formazan (Formazan), the amount of formazan can be measured with a microplate reader Measurements were performed at 570nm. Under normal circumstances, the amount of formazan produced is dir...
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