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Mulberry active polysaccharide and extracting method thereof

An active polysaccharide and extraction method technology, applied in the field of medicine, can solve the problems such as the influence of α-amylase α-glucosidase has not been reported, and the extraction method has not been found, and achieve high-efficiency α-amylase and α-glucosidase activity. , Inhibition of α-amylase and α-glucosidase activities, super antioxidant effect

Active Publication Date: 2015-10-21
广东普莱健康食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are reports about hot water extraction, ultrasonic extraction and antioxidant activity of mulberry polysaccharides, the hot water extraction, ultrasonic extraction, acid extraction, and alkali extraction methods of the prior art have not found that the extraction methods affect the mulberry polysaccharide molecules. At the same time, the effect of the extraction method on the product's anti-oxidation, inhibition of α-amylase and inhibition of α-glucosidase has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Select black mulberries, dry them at 40°C until the moisture content is lower than 8%, and crush them through an 80-mesh sieve to obtain mulberry powder. Weigh 50g of mulberry powder, add 20 times the mass of mulberry powder in 90wt.% ethanol solution, heat and stir at 80°C for 2h, filter, and dry the filter residue at 60°C. Add 30 times the mass of water to the dried filter residue, freeze at -60°C for 4 hours, and thaw at 25°C for 2 hours. The mulberry and water mixture after the thawing process was heated and stirred in a water bath at 80° C. and stirred for 3 h, centrifuged and filtered to obtain the filtrate, then the filtrate was concentrated to 200 ml, and the Sevag reagent (chloroform: n-butanol) of 1 / 3 concentrated liquid volume was added. =4:1, v / v), shaking for 10 min, centrifuging at 4000rmp / min for 20 min, collecting the supernatant, and then deproteinizing by Sevag method, repeated 15 times. Add absolute ethanol so that the final mass concentration of eth...

Embodiment 2

[0045] Select black mulberries, dry them at 60°C until the moisture content is lower than 8%, and crush them through a 60-mesh sieve to obtain mulberry powder. Weigh 50g of mulberry powder, add 10 times the mass of mulberry powder in 90wt.% ethanol solution, heat and stir at 70°C for 4h, filter, and dry the filter residue at 40°C. Add 40 times the mass of water to the dried filter residue, freeze at -70°C for 6h, and thaw at 30°C for 3h. The mulberry and water mixture after the thawing process was heated and stirred in a 90°C water bath for 2h, centrifuged and filtered to obtain the filtrate, then the filtrate was concentrated to 200ml, and the Sevag reagent (chloroform: n-butanol) of 1 / 4 concentrated solution volume was added. =4:1, v / v), shaking for 20 minutes, centrifuging at 5000rmp / min for 10 minutes, collecting the supernatant, and deproteinizing by Sevag method, repeating 10 times. Add absolute ethanol so that the final mass concentration of ethanol in the solution is ...

Embodiment 3

[0048] Select black mulberries, dry them at 50°C until the moisture content is lower than 8%, crush them through a 60-mesh sieve, and obtain mulberry powder. Weigh 50g of mulberry powder, add 10 times the mass of mulberry powder in 90wt.% ethanol solution, heat and stir at 70°C for 3h, filter, and dry the filter residue at 40°C. Add 30 times the mass of water to the dried filter residue, freeze at -60°C for 6 hours, and thaw at 25°C for 3 hours. The mulberry and water mixture after the thawing process was heated and stirred in an 80°C water bath for 3h, centrifuged and filtered to obtain the filtrate, then the filtrate was concentrated to 200ml, and the Sevag reagent (chloroform:n-butanol) of 1 / 5 concentrated solution volume was added =4:1, v / v), shaking for 20 minutes, centrifuging at 5000rmp / min for 10 minutes, collecting the supernatant, and deproteinizing by Sevag method, repeating 10 times. Add absolute ethanol so that the final mass concentration of ethanol in the solut...

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Abstract

The invention discloses a mulberry active polysaccharide and an extracting method thereof. The extracting method comprises: drying mulberries and then carrying out ultrafine grinding-screening and ethanol degreasing pre-treatment; then, carrying out freeze-thawing and wall breaking treatment, hot water extraction, vacuum concentration and protein removal, ethanol precipitation, and freeze-drying, thereby obtaining the mulberry polysaccharide with biological activity, wherein the mulberry polysaccharide with molecular weight ranging from 0.08 million to 0.1 million accounts for 60%-70%, and the mulberry polysaccharide with molecular weight lower than 2000 accounts for 30%-40%. According to the extracting method, freeze-thawing and wall-breaking treatment is carried out on mulberries, so that dissolution of the polysaccharide is facilitated; meanwhile, attention is paid to protection on the polysaccharide in the extraction process; influence on activity of the polysaccharide is reduced; efficient oxidization resistance and effect of inhibiting activities of alpha-amylase and alpha-glucosidase are kept; and the mulberry active polysaccharide can be applied to preparing anti-oxidant and blood-sugar-decreasing health products. The extracting method is simple in process, convenient to operate, high in safety, low in cost and beneficial for realizing industrial application.

Description

technical field [0001] The invention relates to active polysaccharides, in particular to a mulberry active polysaccharide and an extraction method thereof; it belongs to the technical field of medicine. Background technique [0002] Diabetes, as a chronic metabolic disease, has become the third largest disease threatening human life and health in the world due to its high morbidity and mortality. At present, about 5% of people in the world are diagnosed with diabetes, and this number is still increasing year by year. The World Health Organization predicts that the number of people with diabetes will reach 3 billion in 2025 (Jeszka et al. Mulberry leaf extract intake reduces hyperglycaemia in streptozotocin (STZ)‐induced diabetic rats fed high‐fat diet [J], Journal of Functional Foods, 2014, 8:9‐17). Most of the current hypoglycemic drugs are chemically synthesized, such as acarbose, double-arc drugs, sulfonylureas, etc., will bring certain side effects in the process of cli...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P3/10
Inventor 扶雄陈春刘瑞海
Owner 广东普莱健康食品有限公司
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