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Mercury-fibrinogen chelate and preparation method therefor and application thereof

A fibrinogen and chelate technology, applied in the field of detection, to achieve the effect of improving accuracy and repeatability

Inactive Publication Date: 2015-10-28
上海拜豪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] For the serious problem of mercury pollution, the object of the present invention is to provide a kind of mercury-fibrinogen chelate and preparation method thereof, and establish the qualitative and quantitative detection method of mercury-fibrinogen chelate, so that quantitative detection mercury-fibrinogen The application of fibrinogen chelate in evaluating the degree of mercury pollution in an area

Method used

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  • Mercury-fibrinogen chelate and preparation method therefor and application thereof

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preparation example Construction

[0060] The present invention also provides a kind of preparation method of mercury-fibrinogen chelate, comprises the following steps:

[0061] A) the chelation reaction of mercury and fibrinogen: adding mercury ions to the fibrinogen of human origin for chelation reaction to obtain a reaction solution;

[0062] B) Extraction of purified mercury-fibrinogen chelate: Immunoaffinity chromatography is used to remove unreacted fibrinogen and excess mercury ions in the reaction solution to obtain mercury-fibrinogen chelate, specifically Proceed as follows:

[0063] (1) Dissolving samples: adding physiological saline to the reaction solution obtained through step A) to redissolve the mercury-fibrinogen chelate;

[0064] (2) Equilibrium chromatography column: use the dilution buffer to flush the pipeline of the chromatography column, fill the chromatography column with a filler that can specifically bind to fibrinogen, and continue to use the dilution buffer to balance the chromatogra...

Embodiment 1

[0146] Example 1 : the preparation method of mercury-fibrinogen chelate, comprises the following steps:

[0147] A) the chelation reaction of mercury and fibrinogen: adding mercury ions to the fibrinogen of human origin for chelation reaction to obtain a reaction solution;

[0148] Reagent preparation:

[0149] 1) Borate buffer (0.01M): Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH, and dilute to 500mL.

[0150] 2) Fibrinogen solution: Weigh 4.0mg of fibrinogen and dissolve it in 4.0mL 0.01M pH9.0 borate buffer solution, fully vortex to dissolve, and prepare a 1.0mg / mL protein solution;

[0151] 3) EDTA-NaHCO3 mixed solution: weigh EDTA·2H 2 O 1.86g, NaHCO 3 Dissolve 16.8g in 900mL ultrapure water, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000ml, autoclave, and store at room temperature;

[0152] 4) ITCBE was purchased from Japan Tongren Chemical Research Institute, the article number is M030;

[0153...

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Abstract

The present invention discloses a mercury-fibrinogen chelate, a preparation method therefor and an application thereof. The mercury-fibrinogen chelate is obtained by chelating mercury ions with a fibrinogen by a mercapto and / or cysteine residues. The present invention establishes a qualitative and quantitative detection method for the mercury-fibrinogen chelate, so as to quantitatively detect the mercury-fibrinogen chelate in the application of evaluating the mercury contamination degree of a specific region. By quantitatively detecting the mercury-fibrinogen chelate in human serum in the specific region, the mercury contamination influence on human bodies can be reflected indirectly, and the mercury contamination degree of the specific region can be reflected indirectly thereby. The quantitative detection method for the mercury-fibrinogen chelate established in the present invention has much higher accuracy and greatly improved detection repeatability.

Description

technical field [0001] The invention relates to the detection field, in particular to a mercury-fibrinogen chelate, a preparation method and application thereof. Background technique [0002] The physiological function of fibrinogen (fibrinogen, FG) is mainly to participate in the coagulation process directly as coagulation factor Ⅰ. In the common pathway of blood coagulation, thrombin first cleaves Arg16-Gly17 at the amino terminals of the two Aα chains of fibrinogen to release a pair of fibrin peptide A to form fibrin monomer I; -Gly15 releases a pair of fibrin peptide B to form fibrin monomer II, which exposes the polymerization site of the fibrin monomer and forms an unstable soluble fibrin monomer through non-covalent binding. In activated coagulation factor XIII and Ca 2+ Under the action of fibrin monomers, the fibrin monomers are cross-linked with each other to generate stable soluble fibrin, which surrounds the formed components of blood and forms a firm thrombus....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/75C07K1/22G01N33/86G01N33/96
CPCC07K14/75G01N33/86G01N33/96
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 上海拜豪生物科技有限公司
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