Quality testing method of centipede medicinal materials
A quality detection method, centipede technology, applied in measuring devices, material separation, analysis of materials, etc., can solve the problem of lack of specific thin-layer chromatography identification or content determination, the quality of centipede medicinal materials is difficult to be effectively guaranteed, and it is difficult to effectively detect Centipede quality and other issues, to achieve the effect of simple operation, strong specificity, and controllable conditions
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Embodiment 1
[0023] Embodiment 1 centipede medicinal material quality detection method
[0024] 1) Preparation of reference substance solution: Accurately weigh 3,8-dihydroxyquinoline, dissolve in methanol, and prepare 0.00362mg / mL 3,8-dihydroxyquinoline reference substance solution;
[0025] 2) Preparation of the test solution: take 1 g of the centipede medicinal material powder, accurately weigh it, add 25 mL of methanol, weigh, and ultrasonically treat for 30 minutes, take it out, let it cool, make up the weight, shake well, filter, and use it as the test solution;
[0026] 3) Determination of samples: Accurately draw the reference substance solution and the test solution respectively, inject them into the liquid chromatograph, and measure them. Wavelength 252nm; column temperature: 35°C; injection volume 5μL; mobile phase: methanol-10mmol / L potassium dihydrogen phosphate = 28:64.
[0027] 4) Drawing of the standard curve: Precisely draw 1 μL, 2 μL, 4 μL, 8 μL, 10 μL, 12 μL of 3,8-dihy...
Embodiment 2
[0032] Embodiment 2 centipede medical material quality detection method
[0033] 1) Preparation of reference substance solution: Accurately weigh 3,8-dihydroxyquinoline, dissolve in methanol, and prepare 0.00362mg / mL 3,8-dihydroxyquinoline reference substance solution;
[0034] 2) Preparation of the test solution: take 1 g of the centipede medicinal material powder, accurately weigh it, add 25 mL of methanol, weigh, and ultrasonically treat for 30 minutes, take it out, let it cool, make up the weight, shake well, filter, and use it as the test solution;
[0035] 3) Determination of the sample: accurately draw the reference substance solution and the test solution respectively, inject it into the liquid chromatograph, and measure it. Wavelength 252nm; column temperature: 40°C; injection volume 5μL; mobile phase: methanol-10mmol / L potassium dihydrogen phosphate = 32:68.
[0036] Other steps are with embodiment 1.
Embodiment 3
[0037] Embodiment 3 centipede medicinal material quality detection method
[0038] 1) Preparation of reference substance solution: Accurately weigh 3,8-dihydroxyquinoline, dissolve in methanol, and prepare 0.00362mg / mL 3,8-dihydroxyquinoline reference substance solution;
[0039] 2) Preparation of the test solution: take 1 g of the centipede medicinal material powder, accurately weigh it, add 25 mL of methanol, weigh, and ultrasonically treat for 30 minutes, take it out, let it cool, make up the weight, shake well, filter, and use it as the test solution;
[0040]3) Determination of samples: Accurately draw the reference substance solution and the test solution respectively, inject them into the liquid chromatograph, and measure them. Wavelength 252nm; column temperature: 45°C; injection volume 5μL; mobile phase: methanol-10mmol / L potassium dihydrogen phosphate = 34:67.
[0041] Other steps are with embodiment 1.
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